Dilepidid cestode Fuhrmanolepis dubinskyi n. sp. from woodcock, Scolopax rusticola L. in Slovakia is described on the basis of light microscope observations. The new taxon is characterised by medium sized strobila, single crown of ten, 47–53 μm long “diorchid” (wrench-shaped) rostellar hooks. Genital pores alternate irregularly. Number of testes is 20–30 and measurements of cirrus-sac and evaginated cirrus reach 176 − 217 × 18 − 31 and 38 − 56 × 16 − 21 μm, respectively. The uterus is reticular and eggs possess spiny embryophore. F. dubinskyi is differentiated from closely related congeneric species and some other morphologically similar dilepidids.
Altogether 180 specimens of Perca fluviatilis (L.) from Srebarna Lake, North-eastern Bulgaria, were examined for helminths during spring, summer and autumn of 2005 – 2006. Of them, 143 (79.4 %) perch were infected with helminths. Thirteen helminth species were found: metacercariae of Bolbophorus confusus, Diplostomum pseudospathaceum, D. spathaceum, Ichthyocotylurus pileatus, Posthodiplostomum cuticola and Tylodelphys clavata; cestode Proteocephalus percae; acantocephalans Acanthocephalus anguillae and A. lucii and nematode larvae Contracaecum microcephalum, Eustrongilides excisus, E. tubifex and Rhaphidascaris acus. The digenean T. clavata was dominant species of the perch component community. Little seasonal differences were found in the prevalence, abundance and mean infection of helminths. Eustrongilides tubifex reported from the territory of Bulgaria for the first time, represent the new geographical records and new species of Bulgarian helminth fauna.
P. fluviatilis was reported as a new host record for two digeneans, namely Diplostomum pseuspathaceum and Posthodiplostomum cuticola, the cestode Proteocephalus percae, the acantocephalan Acantocephalus anguillae and the nematodes Contracaecum microcephalum and E. tubifex from the territory of Bulgaria. P. fluviatilis was reported as a new host record for D. pseudospathaceum, P. cuticola, C. microcephalum and E. tubifex from Balkan peninsula.
I. Králová-Hromadová, E. Bazsalovicsová, S. Bokorová and V. Hanzelová
Ribosomal internal transcribed spacer 2 (ITS2) structure was studied in monozoic tapeworms Caryophyllaeus laticeps (Pallas, 1781) from freshwater bream Abramis brama (L.) and white-eyed bream Ballerus sapa (Pallas) and Caryophyllaeus brachycollis Janiszewska, 1951 from A. brama and Mediterranean barbel Barbus meridionalis (Risso). Homologous intragenomic ITS2 structure (474 bp) was determined for C. brachycollis from both fish hosts and for C. laticeps from A. brama (486 bp). Contrary to this, divergent intragenomic ITS2 copies (ITS2 paralogues) were detected in C. laticeps from B. sapa. They were mostly induced by different numbers of short repetitive motif (TA)n within the sequences, allowing their assortment into two ITS2 variants (457 and 467 bp). Current data represent first information on ITS2 structure/ITS paralogues in the caryophyllidean family Caryophyllaeidae with focus on their applicability in the molecular taxonomy of the genus Caryophyllaeus.
P. Šalamún, V. Hanzelová, D. Miklisová and T. Brázová
The effect of industrial pollution from chromium ferroalloys production on soil free-living nematode assemblages, c-p groups and generic composition was investigated along 7-km transect. From trace elements (Cr, Cu, Ni and Pb), only chromium exceeded the thresholds for uncontaminated soils (10 mg.kg-1) near the pollution source. In contrast mobilizable fraction of Cr has increased with the distance from the pollution source and was found to be positively correlated with Cox, soil pH, and moisture. Generic richness (nematode diversity) was also higher at remote sites. The low contamination has no significant impact on the nematode communities as illustrated by the c-p groups composition and balanced community structure. Widely applied ecological indices SI and EI also proved maturing ecosystem without any significant stress responses
P. Šalamún, T. Brázová, D. Miklisová and V. Hanzelová
In this study, the effects of arsenic, cadmium, copper and chromium treatments were examined on a nematode community structure and proportion of functional groups in the microcosm for 30 days. The toxic effects on the nematode community did not correspond with metals mobility (EDTA extraction) in soil as it was expected. The most toxic element with a significant degradation of community structure was chromium (low mobile), which negatively affected almost all observed ecological parameters (abundance, diversity and ecological indices). On the other hand, cadmium and arsenic influence was negligible even in the plots treated with the highest concentrations and the communities resembled to the control samples. Copper showed a stimulative effect on the community under low concentration (40 mg.kg-1), while under higher concentrations the stimulation was replaced by stress responses. The widely used ecological indices, such as the Maturity Index 2-5, Structure Index, and Shannon-Weaver Index and c-p groups showed the best bioindication potential among nematode parameters.