Life Cycle of the Trematode Notocotylus intestinalis (Digenea, Notocotylidae) under Natural Conditions in Primorye Region (Russia)
The redia, cercaria, adolescaria and adult trematodes Notocotylus intestinalis Tubangui, 1932 are described. It was established that under natural conditions of Primorye Region, the first intermediate hosts of this trematode are snails Parafossarulus manchouricus and P. spiridonovi. Adult worms were grown in the chicken.
Life Cycle of the Trematode Neodiplostomum oriolinum (Diplostomatidae) under Natural Conditions in Primorye Region (Russia)
The life cycle and developmental stages of the trematode Neodiplostomum oriolinum Oschmarin, 1963 are described. It was experimentally established that under natural conditions of Primorye Region, the first intermediate hosts of this trematode are snails Polypylis semiglobosa, Helicorbis sujfunensis, and the second are tadpoles of Rana dybowskii. Adult worms were developed in chicken and rat. Based on comparative morphological analysis of adult worms developed under experimental conditions and data given in P. G. Oshmarin (1963) paper it is assumed that N. oriolinum and N. paraoriolinum Oschmarin, 1963 are synonyms.
New cercariae similar to those of Paracoenogonimus (Cyathocotylidae, Prohemistomatinae), have been found in gastropods Amuropaludina and Cipangopaludina from reservoirs of the Primorsky Region (southern Far East of Russia). Two species of freshwater fishes (Pseudorasbora parva and Perccottus glenii) were experimentally-infected second intermediate hosts. Adults reared in chicken, Gallus gallus dom. They belong to a new species, Paracoenogonimus ussuriensis sp. n. They differ from those of P. szidati (Anderson, 1914) and Mesostephanus appendiculatus (Ciurea, 1916) (=P. skworzowi (Petrov, 1950) sensu Sudarikov 1961) by the size of the body, pharynx and ovary and, further, from the latter by the size of the suckers and testes. Adults differ from P. ovatus Katsurada, 1914 by the smaller dimensions of the oral sucker, pharynx and testes, and also by the absence of anterior interval between vitelline follicles.
Specimens of the snails Anisus centrifugops were infected with two types of cercariae relating to the genus Diplodiscus, differing from each other by body and organ sizes were found in a pond in the territory of Vladivostok (Primorsky region, Russia). Further study of their morphology and life cycles allowed us to establish that these flukes belong to Diplodiscus japonicus () and D. mehrai Pande, 1937. Morphological and molecular data confirmed the validity of D. japonicus which had previously been synonymized with D. amphichrus Tubangui, 1933 and D. mehrai Pande, 1937.
For first time, 3 species of prosobranchial snails (Bithynia fuchsiana, Parafossarulus striatulus (Bithyniidae) and Melanoides tuberculata (Thiaridae)) from North Vietnam were studied for presence of the parthenitae and cercariae of Trematoda. The morphology of 12 species of larvae belonging to 8 families (Cyathocotylidae, Pleurogenidae, Lecithodendriidae, Paramphistomidae, Heterophyidae, Notocotylidae, Psilostomidae and Echinostomatidae) was described. Data on the life cycles of 3 species (Echinochasmus japonicus, Notocotylus intestinalis and Sphaeridiotrema monorchis) as well as molecular data on 28S rDNA were obtained. There was no intraspecific variability found in any of the sequences examined. The 28S rDNA sequence length determined for N. intestinalis was 1315 bp, while that of S. monorchis was 1292 bp, and that of E. japonicus was 1288 bp.