I. Šulla, V. Balik, J. Petrovičová, V. Almášiová, K. Holovská and Z. Oroszová
Spinal cord injuries (SCI) with their tragic consequences belong to the most serious pathological conditions. That is why they have stimulated basic research workers, as well as health care practitioners, to search for an effective treatment for decades. Animal experimental models have been essential in these efforts. We have jointly decided to test and standardize one of the spinal cord injury compression models in rats. Twentythree adult female Wistar rats weighing 250-320 g were utilized. Employing general anaesthesia along with a mixture of sevoflurane with O2, 2 rats (sham controls) had their vertebral arch of either Th8 or Th9 vertebra removed (laminectomy). The other 21 experimental rats with similar laminectomies were divided into 3 subgroups (n = 7) which received compression impact forces of 30, 40 or 50 g (subgroups-1, -2, and -3, respectively) applied on their exposed spinal medulla for 15 minutes. All rats were observed for 28 days after the experimental procedure and their motor functions were assessed by the Basso, Beattie, Bresnahan (BBB) test 6 hours, 7, 21 and 28 days after the simulated SCI. All 23 rats survived the surgical procedures. The control rats were without any neurological deficits. There were, in every experimental subgroup, 1 or 2 rats with extreme BBB scores. So the rats with the maximum and minimum BBB values were excluded. Then, the results acquired in the residual 5 rats in each group were averaged and statistically analysed by the Tukey multiple comparisons test. Statistically significant intersubgroup differences were found at all survival times equal to or longer than 7 post SCI days. The goal of the SCI experiment was to generate a reproducible and reliable, submaximal spinal cord trauma model. The statistical analyses demonstrated that this objective was best achieved in the subgroup-2 with the 40 g compression.
J. Novotný, P. Reichel, K. Bárdová, P. Kyzeková and V. Almášiová
Clinoptilolite (Cp) is the most common and suitable natural zeolite type for many commercial and industrial applications. Recent studies have also shown a high potential of clinoptilolite in various medical applications. The aim of our study was to evaluate the effect of long-term peroral administration of clinoptilolite on appetites, the consistency of faeces, and the histopathology of the intestines of growing pigs. Fourteen Landrace × Large White crossbred pigs of both genders, a few days after weaning (12.95 kg b. w.), were divided into two equal groups. The control group was fed with a basal feed mixture, and the experimental group with a feed mixture supplemented with 2 % of natural zeolite (the commercial preparation “ZeoFeed”). The appetite, clinical state and consistency of the faeces were assessed every day. The blood samples were collected on days 0, 21, and 42 of the experiment. Histological examinations of the intestines from the control and experimental animals were carried out at the end of the experiments. The supplementation of 2 % Cp did not affect neither the appetite nor the clinical state of the pigs. The faecal consistency score in the experimental animals was 18.82 % lower than that of the control piglets. The histopathological evaluations showed protective evidence of the Cp on the intestinal tract wall in the duodenum and jejunum.