The aim of the study was to analyze the microbiological and physico-chemical composition of sewage sludge from the food industry. The research material was dewatered sewage sludge with and without lime, derived from the water and drink production plant in the Opole region, obtained for testing in March 2013. Physico-chemical analysis included the determination of: pH, temperature of sludge, sedimentation properties, the dry weight, mineral substances, biogenic elements and heavy metals. Microbiological evaluation included quantitative and qualitative determination of mesophilic, psychrophilic and potentially pathogenic microorganisms. Sewage sludge with and without lime characterized similar physico-chemical parameters. However, the addition of lime to the sludge led to a change in ratios of different groups of microorganisms. In the sludge with lime, we observed reduction in the number of mesophilic bacteria and yeast, and total inhibition of psychrophilic bacteria and fungi. Knowledge of the physico-chemical parameters and microbiological composition of the sewage sludge is needed to determine the directions of their development
The aim of the study was to evaluate the drug resistance of Enterococcus faecalis and Enterococcus faecium isolated from different types of poultry waste. The study material consisted of feather samples (duck, turkey, chicken), sludge and centrifuge sediment, originating from three poultry farms. The study was conducted in two stages; isolation and identification of Enterococcus bacteria from the waste and evaluation of their drug resistance using Kirby-Bauer method. Contamination of the poultry waste with Enterococcus isolates included E. faecium species (79 %) and E. faecalis (21 %). The most contaminated were sludge and sediment from the centrifuge as well as chicken feathers, irrespective of the place and time of sampling. Tested isolates showed multiple resistance and similar reaction to all antibiotics used in the study and E. faecalis strain was more resistant. Enterococcus isolates showed the highest resistance to streptogramins, carbapenems, fluoroquinones, aminoglycosides and penicillins, and the lowest for nitrofurantions and phenicols.
The soil contaminated with petroleum products must be excluded from the crops and treated to reclamation processes. Natural processes of decomposition of hydrocarbon compounds go very slow, so it is necessary to use bioaugumentation or stimulation in order to accelerate the return of the soil to high culture. In this study the effect of hydrogen peroxide on the process of cleaning soil strongly contaminated with pertochemicals was investigated. For this purpose, a pot experiment lasting 60 days was carried. The dynamics of changes in the population of filamentous fungi, yeasts and bacteria were examined and also content of aliphatic hydrocarbons (n-alkanes), monoaromatic and polycyclic aromatic hydrocarbons (PAHs). Experimental use of hydrogen peroxide in the process of biodegradation of petroleum compounds assisted in the analyzed soil led to an increase of the number of grampositive bacteria during the test. Stimulation of oil products biodegradation by hydrogen peroxide also increased by 35% decomposition efficiency of aliphatic hydrocarbons (C8-C40) and about 50% PAH’s in comparison to control samples without hydrogen peroxide. There was no influence of hydrogen peroxide on the content of monoaromatic hydrocarbons (BTEX) with respect to controls, although in the end of experiment, the total concentration decreased by about 50% compared to the initial content.
Therefore the carried out study aimed at determination of the effect of high-calcium brown coal ash and compost being produced from municipal sewage sludge on the content and bioaccumulation of heavy metals in potato tubers, wheat grains and rapeseeds during a three-year period. Rapeseeds contained most Cd whereas wheat rains less. Potato tubers, wheat grains and rapeseeds contained more Mn, Ni and Zn in the fertilization objects with municipal sewage sludge with or without coal ash and compared to those where calcium carbonate or coal ash had been introduced into the soil at a dose corresponding to 1.5 Mg CaO · ha–1 at the beginning of this study. Differences in the Mn, Ni and Zn contents in test plants between the fertilization objects with sewage sludge of with and without addition of ash were not significant.
The activity of soil microorganisms affects soil fertility and structure, what leads to satisfactory crop yields, thanks to changes in the decay of organic matter. Their activity may be impaired as a result of application by farmers both fertilizers and pesticides. The degradation of cellulosic biomass represents an important part of the carbon cycle within the biosphere. Cellulolitic microorganisms are responsible for this decomposition, as they produced the enzymes of the cellulase complex. The aim of the study was to compare the cellulolytic activity of soil with Roundup and modified using urea phosphate and / or manure. The pot experiment was conducted under laboratory conditions. The studied material was brown soil (pH of 5.5) fertilized with manure and/or urea phosphate. To each earthenware vase were fed 0.5 kg of the tested soil supplemented with 1% carboxymethyl cellulose. The cellulolytic activities of soil in different experimental variants were expressed in % of C using a colorimetric Petkov method. Modification of soil cellulolytic activity varied considerably depending on the additive manure and/or urea phosphate and Roundup. The lowest soil cellulolytic activity was observed in the presence of manure and urea phosphate, and the highest in the soil with manure and Roundup. The objects of Roundup independently of the other additives urea phosphate stimulate the activity of the cellulolytic microflora compared to objects containing only urea phosphate. The presence of manure in soil treated with Roundup clearly accelerated degradation of cellulose, what can be used in agriculture in the degradation of crop residues. The study shows that pesticides and fertilizers have a big impact on cellulolytic activity in soil. Changes in cellulolytic activity can be used as an ecological indicator of soil pollution level.