Curcumin has been proved to inhibit cell proliferation and induce cell apoptosis in non-small cell lung cancer (NSCLC). However, little is known about antimetastatic effects and molecular mechanisms of curcumin in NSCLC. In this study, we investigated the involvement of miR-206 in curcumin’s anti-invasion and anti-migration in NSCLC. Cell proliferation was determined by MTT assay. Cell migration and invasion were analyzed by wound healing assay and transwell assay. MiRNA-206 expression was detected by real-time PCR. Western blot was used to detect the protein expression of PI3K/AKT/mTOR signaling pathway. Curcumin significantly inhibited migration and invasion in A549 cells, accompanied by significantly elevated miR-206 expression. Overexpression of miR-206 could inhibit migration and invasion of A549 cells, but it could also significantly decrease the phosphorylation levels of mTOR and AKT. The inhibition of miR-206 promoted cell migration, invasion and increased the phosphorylation level of mTOR and AKT. Furthermore, miR-206 mimics improved the inhibitory effects of curcumin on cell migration, invasion and the phosphorylation level of mTOR and AKT in A549 cells. On the contrary, MiR-206 inhibitors reversed the inhibitory effects of curcumin on cell migration, invasion and the phosphorylation level of mTOR and AKT. In conclusion, curcumin inhibited cell invasion and migration in NSCLC by elevating the expression of miR-206 which further suppressed the activation of the PI3K/AKT/mTOR pathway.
Ten mixed-breed female dogs were used in the study. Abdominal wall lifting was performed with a sterilised cotton strip. Four portal sites were used to complete gasless laparoscopic ovariohysterectomy (GLOHE) procedures. The proper and suspensor ligament, ovarian pedicle, and broad ligament of the uterus, uterine artery and vein were coagulated using bipolar electrocoagulation. After the uterine body was coagulated and cut, the end of the cervix was ligated with a loop suture. Blood samples were obtained before the surgery, immediately after the procedure, and on 1, 3, and 5 d postoperatively for the determination of interleukin-6 (IL-6) and C-reactive protein (CRP). No significant surgical complications occurred. After gasless laparoscopic ovariohysterectomy, a significantly higher serum IL-6 level was found immediately after the surgery and 1 d postoperatively when compared with the level observed after traditional laparoscopic ovariohysterectomy (LOHE). However, no significant differences were observed in CRP level between GLOHE and LOHE. GLOHE proved to be a safe and feasible procedure in bitches.
A novel effi cient adsorbent, alkali-pretreated Paeonia ostii seed coats (AP-PSC), was investigated for the removal of methylene blue (MB) dye from solution. Orthogonal array design was applied to optimize the process parameters viz. alkali concentration, liquid-solid ratio (LSR) and pretreatment time. The results revealed that the optimal pretreatment conditions were at 0.8% (w/w) NaOH with LSR of 0.35 L g-1 treating for 50 min. Equilibrium and kinetic studies indicated that Langmuir isotherm and Pseudo-second-order models described the experimental data well. The maximum adsorption capability was of 368.2 mg g-1 for MB at 25oC. Thermodynamic parameters suggested that the AP-PSC adsorption process was physical, endothermic and spontaneous. Furthermore, the adsorption process was infl uenced by several interactive mechanisms, including ion-exchange, as well as Van der Waals forces and hydrogen bonds that occur concomitantly. It was concluded that AP-PSC may be potential as an effi cient adsorbent to remove MB from solution.
The path following control problem of the underactuated unmanned surface vessel (USV) is studied in this paper. An improved line-of-sight (LOS) guidance algorithm is proposed which can adjust adaptively based on the path following error. The global asymptotically stable path following controller is designed based on the nonlinear backstepping method and the Lyapunov stability theory. Firstly, the USV path following error model is established in the Serret-Frenet (SF) coordinate frame. The path following error in the inertial coordinate frame is transformed into the SF coordinate frame, which is used to define the path following control problem. Secondly, inspired by the traditional LOS guidance algorithm, the longitudinal path following error in the SF coordinate frame is introduced into the improved LOS guidance algorithm. This allows the algorithm to adjust adaptively to the desired path. Thirdly, in order to solve the underactuated problem of the USV path following control system, the tangential velocity of the desired path is designed as a virtual input. The underactuated problem is converted to a virtual fully actuated problem by designing the virtual control law for the tangential velocity. Finally, by combining backstepping design principles and the Lyapunov stability theory, the longitudinal thrust control law and the yaw torque control law are designed for the underactuated USV. Meanwhile, the global asymptotic stability of the path following error is proved. Simulation experiments demonstrate the effectiveness and reliability of the improved LOS guidance algorithm and the path following controller.
Side scan sonar measurement platform, affected by underwater environment and its own motion precision, inevitably has posture and motion disturbance, which greatly affects accuracy of geomorphic image formation. It is difficult to sensitively and accurately capture these underwater disturbances by relying on auxiliary navigation devices. In this paper, we propose a method to invert motion and posture information of the measurement platform by using the matching relation between the strip images. The inversion algorithm is the key link in the image mosaic frame of side scan sonar, and the acquired motion posture information can effectively improve seabed topography and plotting accuracy and stability. In this paper, we first analyze influence of platform motion and posture on side scan sonar mapping, and establish the correlation model between motion, posture information and strip image matching information. Then, based on the model, a reverse neural network is established. Based on input, output of neural network, design of and test data set, a motion posture inversion mechanism based on strip image matching information is established. Accuracy and validity of the algorithm are verified by the experimental results.
First principles calculations have been performed to investigate the structure, electronic and optical properties of Y3Fe5O12. Both the cubic and trigonal phases have been considered in our calculation. The calculated structural parameters are slightly larger than the experimental values. The band structures show that Y3Fe5O12 in cubic and trigonal phases have direct band gaps of 0.65 and 0.17 eV. The calculations of dielectric function, absorption, extinction coefficient, refractive index, energy loss function and reflectivity are presented.
This paper presents a state feedback based backstepping control algorithm to address the trajectory tracking problem of an underactuated Unmanned Surface Vessel (USV) in the horizontal plane. A nonlinear three Degree of Freedom (DOF) underactuated dynamic model for USV is considered, and trajectory tracking controller that can track both curve trajectory and straight line trajectory with high accuracy is designed as the well known Persistent Exciting (PE) conditions of yaw velocity is completely relaxed in our study. The proposed controller has further been enriched by incorporating an integral action additionally for enhancing the steady state performance and control precision of the USV trajectory tracking control system. Global stability of the overall system is proved by Lyapunov theory and Barbalat’s Lemma, and then simulation experiments are carried out to demonstrate the effectiveness of the controller designed.
This paper investigates an N-policy GI/M/1 queue in a multi-phase service environment with disasters, where the system tends to suffer from disastrous failures while it is in operative service environments, making all present customers leave the system simultaneously and the server stop working completely. As soon as the number of customers in the queue reaches a threshold value, the server resumes its service and moves to the appropriate operative service environment immediately with some probability. We derive the stationary queue length distribution, which is then used for the computation of the Laplace-Stieltjes transform of the sojourn time of an arbitrary customer and the server’s working time in a cycle. In addition, some numerical examples are provided to illustrate the impact of several model parameters on the performance measures.
In the field of intelligent crowd video analysis, the prediction of abnormal events in dense crowds is a well-known and challenging problem. By analysing crowd particle collisions and characteristics of individuals in a crowd to follow the general trend of motion, a purpose-driven lattice Boltzmann model (LBM) is proposed. The collision effect in the proposed method is measured according to the variation in crowd particle numbers in the image nodes; characteristics of the crowd following a general trend are incorporated by adjusting the particle directions. The model predicts dense crowd abnormal events in different intervals through iterations of simultaneous streaming and collision steps. Few initial frames of a video are needed to initialize the proposed model and no training procedure is required. Experimental results show that our purpose-driven LBM performs better than most state-of-the-art methods.
The ice jam in a river can significantly change the flow field in winter and early spring. The presence of bridge piers further complicates the hydraulic process by interacting between the ice jam and bridge piers. Using the data collected from experiments in a laboratory flume, the evolution of an ice jam around bridge piers having three different diameters has been investigated in this study. Compared to results without-pier, it was found that the formation of an ice jam in the downstream of bridge pier is faster than that in the upstream. The thickness distribution of the ice jam shows clearly different characteristics in front and behind of bridge piers at different stages of the ice jam.