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  • Author: Sibel Söylemez x
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Measuring plasma ferritin levels with two different methods: A comparison of roche cobas E601 versus roche cobas C501 (integrated modular system roche cobas 6000)

Summary

Background

The aim of our study is to compare plasma ferritin levels found to be high or low in terms of reference range by means of electrochemiluminescence (ECLIA) and immunoturbidimetric method and to examine whether they can be used interchangeably.

Methods

84 patients with high plasma ferritin level and 153 patients with low ferritin level according to the reference range were included in the study. Plasma samples measured in Cobas e601 device with ECLIA were also measured as immunoturbidimetric Cobas c501 device. For method comparison, CLSI EP9-A3 Guideline was used. While the consistency between the methods were specified with Passing-Bablok regression analysis and Spearman cor relation analysis, bias error between the methods (bias%) was determined through Bland-Altman analysis.

Results

Both high and low plasma ferritin levels measured with Cobas e601 module and determined high in terms of reference range were compared with the results found with cobas c501 module. The difference was found to be statistically significant (p<0.001). According to regression and correlation (for low plasma ferritin levels; r: 0.993, p<0.001, for high plasma ferritin levels; r: 0.966, p<0.001) results, the methods were in consistency with each other. Additionally, while the bias% value was found to be 10.4% for low plasma ferritin levels, it was found to be 12.6% for high ferritin levels.

Conclusions

Accordingly, we believe that, comparison with more samples especially in terms of different clinical decision levels is required in order to examine inter changeable use of immunoturbidimetric method in integrated devices and ECLIA.

Open access
Evaluation of paraoxonase, arylesterase, and homocysteine thiolactonase activities in patients with diabetes and incipient diabetes nephropathy

Summary

Background

The aim of this study is to examine the relationship among the changes in activities of paraoxonase (PON), arylesterase (ARE) and homocysteine thiolactonase (HTLase) enzyme having antioxidant properties and the development of diabetic nephropathy (DN), one of the most common complications of diabetes.

Methods

Normoalbuminuric type-2 diabetic patients (Group II, n=100), microalbuminuric type 2 diabetic patients (Group III, n=100) and the control group (Group I, n=100) were included in the study. The age and gender of the patient groups matched with the age and gender of the control group. HTLase, PON and ARE enzyme activities were measured by the spectrophotometric method using a g-thiobutyrinolactone, paraoxon, and phenylacetate substrates respectively. In this study, an autoanalyzer application was developed in order to measure HTLase enzyme activity for the first time.

Results

Serum HTLase, ARE and PON activities of Group III and Group II were significantly low compared to HTLase, ARE and PON results of Group I (p<0.05).

Conclusions

Based on our results, PON, ARE and HTLase enzyme activities were found to be decreased due to the increase in the degree of DN.

Open access