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Open access

Chaoliang Leng, Hongliang Zhang, Yunchao Kan, Lunguang Yao, Mingliang Li, Hongyue Zhai, Zhen Li, Chunxiao Liu, Hongfei Shi, Jun Ji, Reng Qiu and Zhijun Tian

Abstract

Introduction: In 2014–2015, the epidemic of classical swine fever (CSF) occurred in many large-scale pig farms in different provinces of China, and a subgenotype 2.1d of CSF virus (CSFV) was newly identified.

Material and Methods: The phylogenetic relationship, genetic diversity, and epidemic status of the 2014–2015 CSFV isolates, 18 new CSFV isolates collected in 2015, and 43 other strains isolated in 2014–2015 were fully analysed, together with 163 CSFV reference isolates.

Results: Fifty-two 2014–2015 isolates belonged to subgenotype 2.1d and nine other isolates belonged to subgenotype 2.1b. The two subgenotype isolates showed unique molecular characteristics. Furthermore, the 2.1d isolates were found to possibly diverge from 2.1b isolates.

Conclusion: This study suggests that the Chinese CSFVs will remain pandemic.

Open access

Lu-Lu Wang, Shi-Ying Lu, Pan Hu, Bao-Quan Fu, Yan-Song Li, Fei-Fei Zhai, Dan-Di Ju, Shi-Jun Zhang, Bing Su, Yu Zhou, Zeng-Shan Liu and Hong-Lin Ren

Abstract

Introduction: Peroxiredoxin 6 (Prdx6) is a bifunctional protein with glutathione peroxidase activity and phospholipase A2 activity. Previous studies have shown a significant positive correlation between the intracellular survival ability of Brucella and Prdx6. Here, the Prdx6 enzyme with a single activity was constructed to facilitate study of the relationship between the single function of Prdx6 and Brucella infection.

Material and Methods: The target open reading frame (ORF) DNAs of Prdx6 with a single active centre were prepared using gene splicing by overlap extension PCR (SOE-PCR), and the recombinant eukaryotic expression plasmids inserted by Prdx6 with the single activity centre were constructed and transfected into murine Raw264.7 macrophages. The glutathione peroxidase activity and phospholipase A2 activity of the constructed Prdx6 were examined.

Results: The core centres (Ser32 and Cys47) of Prdx6 were successfully mutated by changing the 94th nucleotide from T to G and the 140th nucleotide from G to C in the two enzyme activity cores, respectively. The constructed recombinant plasmids of Prdx6 with the single active centre were transfected into murine macrophages showing the expected single functional enzyme activity, which MJ33 or mercaptosuccinate inhibitors were able to inhibit.

Conclusion: The constructed mutants of Prdx6 with the single activity cores will be a benefit to further study of the biological function of Prdx6 with different enzyme activity.

Open access

Li-jun Peng, Jin-sheng Guo, Zhe Zhang, Li-li Liu, Yi-rong Cao, Hong Shi, Jian Wang, Scott L. Friedman, John J. Sninsky and Ji-yao Wang

Abstract

Objective Genome-wide association studies (GWAS) have linked many single nucleotide polymorphisms (SNPs) to the outcomes of a variety of liver diseases. The aim of the present study was to evaluate the association of several candidate SNPs with the risk and severity of cirrhosis due to chronic hepatitis B in a Chinese population.

Methods A total of 714 Chinese participants with persistent HBV infection were studied. Patients were divided into cirrhotic (n = 429) and non-cirrhotic (n = 285) groups based on clinical and pathological evidence. The progression rate and severity of liver cirrhosis were evaluated with an arbitrary t-score system. Genotypes of six SNPs in five candidate genes were detected with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The genotypic distributions of the SNPs were compared between the age-matched cirrhotic and non-cirrhotic subjects. The association between the risk of SNPs and the severity and progression rate of cirrhosis was further analyzed.

Results Rs2679757 polymorphism of the antizyme inhibitor 1 (AZIN1) gene and Rs886277 in the transient receptor potential cation channel subfamily M, member 5 gene (TRPM5) were found to be associated with cirrhosis risk in CHB. They were also correlated with the overall severity and progression rate of cirrhosis. Genotype frequencies of other SNPs were not different between the cirrhosis and non-cirrhosis groups.

Conclusions AZIN1 rs2679757 and TRPM5 rs886277 are associated with the risk and the progression rate of HBV-related liver fibrosis in Chinese patients. The emerging SNPs associated with cirrhosis prognosis warrant further clinical validation in other CHB cohorts or ethnic groups, and merit mechanistic studies to reveal their roles in fibrosis progression.

Open access

Jin Guofeng, Zhang Wei, Shi Jun, Yang Zhengwei, Hu Yu, Huang Zhiyong and Tian Gan

Due to the disadvantages of traditional NDT methods for liquid propellant tank corrosion detection, Thermal Wave Nondestructive Testing (ITWNDT) technology was applied. The heat exchange process of thermal wave in corrosion tank was simulated by the numerical method. Parameters of TWNDT as the best detection time (tbest), the maximum surface temperature difference (ΔTmax), and the temperature difference holding time (τΔT>0.1) were discussed as the targets. Based on these parameters, factors influencing the detection results of tank materials, dressed liquid (also considered as the corrosion product), pit characters (depth and size), heat flux and thermal excitation time length (pulsed width), environmental conditions and other factors were analyzed. Simulation results show that ITWNDT can identify the defect depth, size and position rapidly and effectively. Material properties of the tank were influencing the tbest, ΔTmax and τΔT>0.1, while the dressed liquid, thermal excitation parameters and the conditions of environment do not influence the tbest. Pit characters of the depth and size have close relationship with tbest and ΔTmax, therefore, for a tank with certain material and certain liquid dressed in, the pit corrosion damage can be accurately evaluated.