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Srdjan Mijatović, Tamara Alempijević, Branislava Stefanović, Vasilije Jeremić, Slobodan Krstić, Nikola Radmanović, Sanja Jovanović and Branislav Stefanović


Background: Oxidative stress represents tissue damage caused by reactive forms of oxygen and nitrogen due to the inability of antioxidant mechanisms to reduce reactive forms into more stable ones. The aim of the study was to evaluate the influence of surgical trauma on nitric oxide (NO) and nitrotyrosine (NT) values in patients undergoing conventional and laparoscopic cholecystectomy.

Methods: A prospective study included sixty patients from the Department of Emergency Surgery, Clinical Centre of Serbia who were operated for gallstone related chronic cholecystitis. All the patients enrolled in the study underwent cholecystectomy; the first group was operated conventionally (30 patients - control group), while the second group was operated laparoscopically (30 patients - treatment group).

Results: There were no statistically significant differences in the values of NO and its postoperative changes in both groups, the conventionally operated group (p=0.943) and the laparoscopically operated group (p=0.393). We found an increase in NT values 24 hours postoperatively (p=0.000) in the conventionally operated patients, while in the group operated laparoscopically we didn’t find statistically significant changes in the values of NT (conventionally operated group (p=0.943) and laparoscopically operated group (p=0.393)).

Conclusions: In our study, we found a significant increase in NT values 24 hours postoperatively in conventionally operated patients i.e. the control group, vs. the treatment group. Further randomized studies are needed for a better understanding of the impact of surgical trauma on oxidative stress response.

Open access

Snježana Mirković, Katarina Rajković, Sanja Jeremić, Marijana Gavrilović, Ljiljana Tomić, Valentina Arsić Arsenijević and Boro Krstić


The objective of this paper was to assess the antiradical effectiveness of propolis extract (PE) based on 2.2-diphenyl-1-picrylhydrazyl radical (DPPH) bleaching assay kinetic profile. The kinetic profile of scavenging DPPH for PE exhibited one kinetic period characterized by one kinetic constant. The second-order rate constant (k 2) for the oxidation of PE by DPPH, determined for the first time in this study, was 0.17 dm3g−1s−1. The obtained k2 value was compared to that of synthetic antioxidants and natural extracts used in the food industry. Kinetic analysis of PE antiradical effectiveness showed that the k 2 was within the range values for natural colorants of fruit extracts and should be considered as a fast acting natural antioxidant source. The k 2 parameter indicates the extent of oxidation inhibition that is based on all of the kinetic profiles of DPPH bleaching rather than single point measurements. For this reason, the kinetic analysis should become a necessary step for more precise antioxidative characterization of propolis.

Open access

Dragana Popovic, Katarina Lalic, Aleksandra Jotic, Tanja Milicic, Jelena Bogdanovic, Maja Đorđevic, Sanja Stankovic, Veljko Jeremic and Nebojsa M. Lalic


Background: We analyzed cardiovascular inflammatory (C-reactive protein (CRP), interleukin 6 (IL-6)), haemostatic (homocysteine) risk markers in lean and obese patients at admission and acute hyperglicemic crisis (AHC) resolving, involving diabetic ketoacidosis (DKA) and hyperosmolar hyperglycemic state (HHS).

Methods: In that context, we included group A: N=20 obese, B: N=20 lean patients with DKA; C: N=10 obese, D: N=10 lean patients with HHS; E: N=15 obese, F: N=15 lean controls. CRP, IL-6, homocysteine were determined by ELISA.

Results: Our results showed that CRP, IL-6, and homocysteine levels decreased in all groups: (A: p<0.001; B: p<0.001, C: p<0.05; D: p<0.001 mg/L), (A: p<0.001 B: p<0.001, C: p<0.001, D: p<0.01 pg/mL), (A: p<0.001, B: p <0.001; C: p<0.05, D: p=0.001 μmol/L), respectively, at resolving AHC. However, CRP persisted higher (p<0.001, p<0.01), IL-6 lower (p<0.05, p<0.001), while homo cysteine levels turned out to be similar to controls.

Conclusions: AHC is associated with increased inflammatory and hemostatic cardiovascular risk markers. Also, insulin therapy in AHC has had more pronounced favorable effect on IL-6 and homocystein than on CRP.