F. Zigo, A. Adamová, M. Vasiľ, J. Elečko, S. Ondrašovičová, M. Zigová and L. Kudělková
The aim of this study was to evaluate the occurrence of mastitis and its impact on the reproductive parameters in a herd of 180 dairy cows. Based on the herd records 127 cows of Slovak spotted cattle and their crosses with red Holstein were selected for study between 1—2 months after calving. The examination of the health status of the mammary glands consisted of: the clinical examination of the udder, the California mastitis test (CMT) supplemented by the collection of mixed milk samples, and the laboratory examination of bacterial pathogens causing the mastitis. In addition to the mammary investigation, reproduction indicators such as the length of the insemination interval, the service period, the intercalving period and the insemination index were also analyzed. The results of this study indicated: a high incidence of mastitis (41.6 %), especially latent (21.2 %), subclinical (15.7 %) and clinical (4.7 %) forms were most common in the herd. The most frequently isolated bacteria from the infected milk samples were: coagulase negative staphylococci (54.1 %), S. aureus (16.9 %), Streptococcus spp. (15.0 %), A. viridans (7.5 %) and Ent. faecalis (6.4 %). According to the available literature, the optimum values of the intercalving period were 365—400 days, the insemination interval 55—80 days, the insemination index 1.2—2 and the service period 60—110 days. In comparison, our results showed increased, unsatisfactory reproductive values in the group of dairy cows with clinical mastitis. While in healthy cows as well as in groups of cows with latent and subclinical mastitis, all of the reproductive indicators were within the optimal levels.
J. Kachnič, N. Sasáková, I. Papajová, K. Veszelits Laktičová, R. Hromada, J. Harkabus, S. Ondrašovičová and J. Papaj
The study was conducted to investigate the microbiological and parasitical risk related to the disposal of animal manure to soil by storage of raw pig slurry at temperatures 4 °C, 20 °C and 42 °C for 115 days. Plate counts of Salmonella typhimurium and number of devitalized non-embryonated model Ascaris suum eggs were determined on days 0, 7, 12, 22, 32, 40, 55, 90 and 115 of storage. At the same intervals level of selected physico-chemical parameters were determined. Microbiological examination showed that S. typhimurium survived in the slurry for less than 115 days at 4 °C and less than 90 days at 20 °C and 42 °C. Devitalization of A. suum eggs increased with temperature and time of storage, but complete devitalization was not achieved even after 115 days at 42 °C. Physico-chemical parameters showed changes related to decomposition processes, but did not allow us to draw definite conclusion regarding their influence on devitalization of pathogens. The results indicate potential risk to human food chain that can be prevented by strict observation of legislative provisions and appropriate treatment of animal manure.