The distribution of Pratylenchus species associated with wheat crops was investigated in Bohemian region of the Czech Republic. In total twelve localities were sampled. The populations were identified based on morphology and morphometrics, and further characterised based on sequences of the rDNA D2/D3 region and 18S gene. Pratylenchus crenatus was present in two localities and P. neglectus in five localities. At one locality both species were detected. Sequence analysis of 18S and D2/D3 region of three populations of P. crenatus reveal no variation while five populations of P. neglectus differ by 0 to 0.14 % (18S) and 0.17 to 0.50 % (D2/D3).
Longidorus caespiticola was found for the first time from the rhizosphere of apple with two other Longidorus sp. at Žlunice, Czech Republic. Females and males were analyzed morphologically and morphometrically. Four regions (18S, ITS1, ITS2, D2-D3 expansion segments of 28S) of ribosomal DNA and one region (cox1) of mitochondrial DNA were sequenced and analyzed and compared with a population from the Slovak Republic. The partial mitochondrial cytochrome c oxidase subunit 1 (cox1) gene showed relatively high genetic variation compared with ribosomal DNA between the Czech and Slovakian populations.
Morphometrics of first, second and fourth juvenile stages and females of Xiphinema dentatum Sturhan, 1978 are presented from the Czech Republic. The species was found associated with Xiphinema spp., Longidorus intermedius and Longidorus spp. under a forest soil.
Soil samples under the rhizosphere of Brasicca napus were collected from three localities (Bílé Podolí, Prague, Kylešovice). All three localities were positive for the presence of Aphelenchus avenae. Morphological and molecular features of A. avenae are presented in this research note.
A survey has been carried out to study the occurrence and distribution of Paratrichodorus pachydermus nematodes in the Czech Republic in a range of habitats (orchards, forests, vineyards, strawberry and river bank). A total 208 sites were surveyed and 19 sites were found positive for P. pachydermus. This species is a new record for Czech nematofauna.
A nematode survey was carried out in South Moravia and Bohemia (Czech Republic) to assess the occurrence of Xiphinema in the rhizosphere of fruit orchards. Sixty six orchards in South Moravia and seven in Bohemia were studied during the years 2003 and 2004. Four Xiphinema species (X. diversicaudatum, X. pachtaicum, X. simile and X. vuittenezi) were recorded. X. simile constitutes a first record for the nematodes fauna of the Czech Republic.
Soil samples under the rhizosphere of Brasicca napus were collected from three localities (Bílé Podolí, Prague, Kylešovice). Two localities Prague and Kylešovice were positive for the presence of Pratylenchus neglectus. The species was identified using morphological features and the morphological identification was verified by using published species-specific primers and by sequencing 18S and 28S genes of ribosomal DNA.
The occurrence and geographic distribution of the genus Longidorus was investigated during a nematological survey of Bohemia and South Moravia between 2003 and 2006. Soil samples were collected from 139 different fruit orchards and vineyards. Three Longidorus species (L. elongatus, L. euonymus and L. leptocephalus) were recorded. L. euonymus constitutes a new record for the Czech nematofauna.
A survey has been carried out to study the occurrence and distribution of Trichodorus primitivus, T. sparsus and T. viruliferus in the Czech Republic under the rhizosphere of orchards, forests, vineyards and strawberry. Total 208 sites were surveyed and only 29 sites were found positive for these species. All three species are reported in the Czech Republic for the first time.