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  • Author: R. Kořínek x
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Characterization of the Embryogenic Tissue of the Norway Spruce Including a Transition Layer between the Tissue and the Culture Medium by Magnetic Resonance Imaging

Abstract

The paper describes the visualization of the cells (ESEs) and mucilage (ECMSN) in an embryogenic tissue via magnetic resonance imaging (MRI) relaxometry measurement combined with the subsequent multi-parametric segmentation. The computed relaxometry maps T 1 and T 2 show a thin layer (transition layer) between the culture medium and the embryogenic tissue. The ESEs, mucilage, and transition layer differ in their relaxation times T 1 and T 2; thus, these times can be used to characterize the individual parts within the embryogenic tissue. The observed mean values of the relaxation times T 1 and T 2 of the ESEs, mucilage, and transition layer are as follows: 1469 ± 324 and 53 ± 10 ms, 1784 ± 124 and 74 ± 8 ms, 929 ± 164 and 32 ± 4.7 ms, respectively. The multi-parametric segmentation exploiting the T 1 and T 2 relaxation times as a classifier shows the distribution of the ESEs and mucilage within the embryogenic tissue. The discussed T 1 and T 2 indicators can be utilized to characterize both the growth-related changes in an embryogenic tissue and the effect of biotic/abiotic stresses, thus potentially becoming a distinctive indicator of the state of any examined embryogenic tissue.

Open access
MRI-Based Visualization of the Relaxation Times of Early Somatic Embryos

Abstract

The large set of scientific activities supported by MRI includes, among others, the research of water and mineral compounds transported within a plant, the investigation of cellular processes, and the examination of the growth and development of plants. MRI is a method of major importance for the measurement of early somatic embryos (ESE) during cultivation, and in this respect it offers several significant benefits discussed within this paper. We present the following procedures: non-destructive measurement of the volume and content of water during cultivation; exact three-dimensional differentiation between the ESEs and the medium; investigation of the influence of ions and the change of relaxation times during cultivation; and multiparametric segmentation of MR images to differentiate between embryogenic and non-embryogenic cells. An interesting technique consists in two-parameter imaging of the relaxation times of the callus; this method is characterized by tissue changes during cultivation at a microscopic level, which can be monitored non-destructively.

Open access