In the 21st century, numerous complex challenges in education and health care have come to the fore, among them: 1) how to implement the ecological approach in the education process and health care practice; 2) how to implement study programmes in line with the education trends for sustainable development and the process of formation and development of the ecological competence.
The results of this research refer only to the content analysis of the essays written by the first and second year students of the programs in Nursing and Medicine on completing the study course The Ecological Approach in Patient Care. To increase the credibility and validity of the obtained results of the content analysis, the triangulation method has been applied to include quantitative and qualitative data. The research has looked into the concept of ecology, its application and importance in developing the ecological consciousness of students during their transformation from I-Ego to I-Eco in the process of developing their ecological competence (Salīte, 1998).
Ance Bogdanova, Uldis Bērziņš, Ruta Brūvere, Guļšena Eivazova and Tatjana Kozlovska
Adipose-derived stem cells cultured in autologous serum maintain the characteristics of mesenchymal stem cells
Human adipose tissue is known to be an attractive and readily available source of mesenchymal stem cells (MSC), which are becoming increasingly popular for application in regenerative medicine. Most of the protocols currently used for in vitro expansion of MSC include fetal bovine serum (FBS) supplementation. When MSC are cultured in such a way for clinical applications this rises concerns about immunogenicity of FBS proteins. A possible solution to this problem is the use of autologous serum (AS) instead of FBS. In this study we investigated whether adipose-derived stem cells (ADSC), cultivated in medium containing AS, maintain characteristics of MSC. The results show that the obtained ADSC were plastic adherent, rapidly dividing (doubling time 40 ± 4 hours), spindle-shaped cells with fibroblastoid morphology and exhibited normal karyotype. No less than 95% of the obtained cells displayed MSC surface markers, including CD73, CD90 and CD105, but showed no expression of the hematopoietic markers CD34 and CD45. ADSC cultured in the presence of AS underwent in vitro differentiation into adipocytes, osteoblasts and chondroblasts, confirmed by Oil Red O, Alizarin Red S and Alcian Blue stains, respectively. These findings suggest that ADSC may be expanded in the AS without the loss of characteristics of MSC.