Supercooling Points of Apis Mellifera Ligustica when Performing Different Age-Related Tasks
In order to study the cold resistance of honey bees, the authors systematically investigated the supercooling points (SCPs) of Apis mellifera ligustica worker bees performing different age-related tasks. There were statistically significant differences in SCPs between worker bees performing different activities (P<0.05). The nectar-water collector had the highest SCP temperature (-3.16°C), highest water content (74.85%) and lowest crude fat content (6.13%). The soldier had the lowest SCP temperature (-6.40°C), relatively lower water content (70.33%) and higher crude fat content (7.28%). No significant difference was found in the SCPs of workers of different ages. Winter bees did not differ from summer bees in their SCPs. The relatively higher SCPs from different kinds of individual bees suggest that honey bees do not mainly rely on their low SCPs for their cold resistance.
The aim of this study was to explore the safe and effective method of expectoration in the preoperative period of patients with lung cancer resection and to promote the rehabilitation of patients.
A total of 100 cases of lung cancer patients undergoing elective surgery were divided into the observation group and the control group, with 50 cases in each group. The control group was treated with vibration expectoration vest for expectoration during the perioperative period, and the observation group was treated with respiratory function exerciser that has expectoration function in the perioperative period, three times a day, and the effect was evaluated after 5 days.
The number of patients in the observation group after the first expectoration time was significantly less than that of the control group (P<0.001). Pain score, pulmonary atelectasis, and pulmonary infection rate of the observation group were significantly lower than those of the control group; the hospitalization time was significantly shorter than that of the control group; and the difference was statistically significant (P<0.05).
Lobectomy for lung cancer patients with perioperative respiratory training for respiratory function exercise, compared with conventional methods, is helpful for postoperative expectoration and to reduce the incidence of adverse events.
The aim of this study was to investigate the effect of Radix Glehniae on the migration and invasion abilities of lung cancer cells.
Normal bronchial cell line 16HBE and lung cancer cell line SK-MES-1 were treated with Radix Glehniae extract. Proliferation, migration, and invasion abilities were determined by Cell Counting Kit (CCK)-8, Transwell, and Matrigel assays, respectively. The expression and secretion levels of tissue inhibitor of metalloproteinases 2 were detected by quantitative PCR and enzyme-linked immunosorbent assay, respectively.
Radix Glehniae extract inhibited the migration and invasion abilities of SK-MES-1 cells and enhanced TIMP2 expression and secretion by SK-MES-1 cells, without causing toxicity to 16HBE cells.
Radix Glehniae is useful in lung cancer treatment.
The behavior of boron redistribution in silicon with and without oxide layer after electron beam injection (EBI) was investigated. Special defect shapes were generated on the surface of bare and oxidized silicon wafers. Secondary ion mass spectrometer was used to measure the boron profile. The results showed that after long EBI time, boron tended to be induced from both sides of the transition region between the oxide layer and silicon. For the sample without oxide layer after EBI, boron tended to diffuse towards the surface and its concentration obviously reduced inside the silicon. The results of the study show the potential use of the process in removing boron impurity in silicon.
Objective To investigate the relationship between single nucleotide polymorphisms (SNPs) of the interleukin-4 (IL-4) gene and outcome of hepatitis B virus (HBV) infection in a Chinese Han population.
Methods Total of 501 patients with chronic hepatitis B virus (HBV) infection and 301 controls with selflimiting HBV infection were studied. Three tag SNPs in the IL-4 gene (rs2227284G/T, rs2243283C/G and rs2243288A/G) were genotyped by the Multiplex snapshot technique. The genotype and allele frequencies were calculated and analyzed.
Results The three SNPs showed no significant genotype/allele associations with chronic HBV infection. Overall allele P values were: rs2227284, P = 0.655, odds ratio (OR) [95% confidence interval (CI)] = 1.070 (0.793-1.445); rs2243283, P = 0.849, OR (95% CI) = 0.976 (0.758-1.257); rs2243288, P = 0.659, OR (95% CI) = 1.060 (0.818-1.375). Overall genotype P values were: rs2227284, P = 0.771; rs2243283, P = 0.571; rs2243288, P = 0.902. There were no statistically significant differences between patients with chronic HBV infection and controls. Haplotypes generated by these three SNPs also had no significant differences between the two groups.
Conclusions The three tag SNPs of IL-4 were not associated with the outcome of HBV infection in the Han Chinese population.
Polypropylene/carbon nanotubes (PP/CNTs) nancomposites were prepared with a single screw extruder by adding maleic anhydride-grafted poplypropylene (PP-g-MAH) as compatibilizer to polypropylene (PP) with different amounts of carbon nanotubes (CNTs) in the range of 0.1–0.7 wt.%. Structure and morphology of the prepared samples were examined by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), polarizing light microscopy (PLM) and X-ray diffraction (XRD). The results showed that PP spherulites decreased in size when CNTs were introduced into the polymer. Mechanical properties of the samples were also studied. Tensile tests showed that with increasing amount of CNTs the strain at break decreased whereas the Young’s modulus was improved of 16.41 % to 36.05 % and tensile strength of 36.67 % to 64.70 % compared to pristine PP. The SEM microphotographs showed that majority of the CNTs were dispersed individually and oriented along the shear flow direction.
The objective of this study was to discuss the effect of double perfusion cannula accompanied with low negative pressure drainage in the treatment of complexity of abdominopelvic and perineal infections.
The technology of the double perfusion cannula accompanied with low negative pressure drainage was used to treat complexity of abdominopelvic and perineal infections.
Double perfusion cannula accompanied with low negative pressure drainage can be applied to the treatment of complexity of abdominopelvic and perineal infections. It has an obvious effect on infection control and reduces recovery time.
Double perfusion cannula accompanied with low negative pressure drainage has a good effect on complexity of abdominopelvic and perineal infections; it can be used in wider surgical fields to prevent infections.
High-speed blades form core mechanical components in turbomachines. Research concerning online monitoring of operating states of such blades has drawn increased attention in recent years. To this end, various methods have been devised, of which, the blade tip-timing (BTT) technique is considered the most promising. However, the traditional BTT method is only suitable for constant-speed operations. But in practice, the rotational speed of turbomachine blades is constantly changing under the influence of external factors, which lead to unacceptable errors in measurement. To tackle this problem, a new BTT method based on multi-phases is proposed. A plurality of phases was arranged as evenly as possible on the rotating shaft to determine the rotation speed. Meanwhile, the corresponding virtual reference point was determined in accordance with the number of blades between consecutive phases. Based on these reference points, equations to measure displacement due to blade vibrations were deduced. Finally, mathematical modeling, numerical simulation and experimental tests were performed to verify the validity of the proposed method. Results demonstrate that the error in measurement induced when using the proposed method is less than 1.8 %, which is much lower compared to traditional methods utilized under variable-speed operation.
Flow-injection mass spectrometry (FIMS) coupled with a chemometric method is proposed in this study to profile and distinguish between rhizomes of Smilax glabra (S. glabra) and Smilax china (S. china). The proposed method employed an electrospray-time-of-flight MS. The MS fingerprints were analyzed using principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) with the aid of SIMCA software. Findings showed that the two kinds of samples perfectly fell into their own classes. Further predictive study showed desirable predictability and the tested samples were successfully and reliably identified. The study demonstrated that the proposed method could serve as a powerful tool for distinguishing between S. glabra and S. china.
Objective: To observe the effects of electro-acupuncture (EA) on GRP78 and Caspase-12 gene expression in rats with ischemia- reperfusion injury (IRI) by stimulation on Nei Guan (PC6) and Bai Hui (GV20) points, so that to understand whether or not the protective effects of acupuncture is related to endocytoplasmic reticulum (ER) stressapoptosis passage.
Methods: 50 rats were randomly assigned to five groups (10 in each group): normal control(A), pseudo-operation(B), operation(C), Edaravone(D) and EA(E). The ischemia/reperfusion model of middle cerebral artery occlusion (MCAO) was established by suture embolic method. TUNEL staining method was employed to measure the apoptosis index of nerve cells in rats. Real-time polymerase chain reaction (RT-PCR) was employed to measure the mRNA expression of GRP78 and Caspase-12.
Results: Compared with normal group and pseudo-operation group, the apoptosis indexes and mRNA expression of GRP78 and Caspase-12 in operation group, Edaravone group and EA group were increased, with statistical significance（P＜0.05 or P＜0.01）; compared with operation group, the apoptosis indexes and Caspase-12 mRNA expression in Edaravone group and EA group were decreased（P＜0.05 or P＜0.01）, but GRP78 mRNA expression were increased（P＜0.01）; there were no significant difference between Edaravone group and EA group on the above indexes（P＞0.05).
Conclusion: Acupuncture on Nei Guan and Bai Hui points could effectively suppress the nerve cell apoptosis in cerebral ischemia. The underlying mechanism might be related to upregulation of the ERS-protective GRP78 expression and downregulation of apoptosis-promotion Caspase-12 expression.