Agnieszka Pluta, Tadeusz Robak, Kamil Brzozowski, Barbara Cebula-Obrzut, Agata Majchrzak, Piotr Pluta, Anna Szmigielska-Kapłon, Olga Grzybowska-Izydorczyk, Magdalena Czemerska, Piotr Stelmach, Piotr Smolewski and Agnieszka Wierzbowska
Acute myeloid leukemia (AML) is a heterogeneous, highly malignant neoplasm. Apoptosis is a complex process executed by caspases and suppressed by the inhibitor of apoptosis (IAP) family. Neuronal apoptosis inhibitory protein (NAIP), IAP’s member, may play an exceptional role in the mechanisms of tumors’ resistance to chemotherapy. The aims of the study were to assess the expression of NAIP in leukemic blasts of AML patients using flow cytometry and to evaluate its influence on disease outcome. NAIP expression was found in 106 out of 108 patients. A higher complete response rate was associated with a low expression of NAIP, age < 60 yo, and white blood cell count < 20 G/L (p = 0.009, p = 0.033, and p = 0.076, respectively) in univariate analyses and a low NAIP expression and age < 60 yo (p = 0.025 and p = 0.013, respectively) in multivariate analyses. Longer overall survival (OS) in the univariate analysis was influenced by a low NAIP expression, age < 60 yo, and intensive chemotherapy (p = 0.033, p < 0.001, and p < 0.001, respectively). In the intensively treated group, better OS was observed in patients with age < 60 yo, de novo AML, and a low NAIP expression (p = 0.03, p = 0.024, and p = 0.07, respectively). In multivariate analysis, longer OS was associated with age < 60 yo (p = 0.009) and de novo AML (p = 0.007). In conclusion, we suggest that NAIP might play an adverse role in response to chemotherapy.
Agnieszka Waśkiewicz, Karolina Gromadzka, Jan Bocianowski, Paulina Pluta and Piotr Goliński
The aim of this study was to establish a relation between zearalenone contamination of crops in the Polish province of Wielkopolska and its occurrence in aquatic ecosystems close by the crop fields. Water samples were collected from water bodies such as drainage ditches, wells, or watercourses located in four agricultural areas. Moreover, control water samples were collected from the Bogdanka river, which was located outside the agricultural areas and near an urban area. Cereal samples were collected in the harvest season from each agricultural area close to tested water bodies. Zearalenone (ZEA) was found in all water and cereal samples. The highest concentrations were recorded in the postharvest season (September to October) and the lowest in the winter and spring. Mean ZEA concentrations in water ranged between 1.0 ng L-1 and 80.6 ng L-1, and in cereals from 3.72 ng g-1 to 28.97 ng g-1. Our results confirm that mycotoxins are transported to aquatic systems by rain water through soil.
Monika Olech, Marzena Rola-Łuszczak, Bożenna Kozaczyńska, Piotr Kubiś, Aneta Pluta, Anna Gil and Jacek Kuźmak
In the study, a 122 bp fragment of gag gene encoding immunodominant epitope on capsid protein of small ruminant lentiviruses (SRLVs) found in sheep was amplified by PCR and analysed by SSCP and sequencing. Out of 30 DNA samples, five showed different migration patterns, demonstrating the individual variations within gag sequences, which were confirmed afterwards by sequence analysis. In two samples nucleotide changes yielded amino acid substitutions highlighting the conservative nature of gag encoded immunoreactive epitope but also potencial insensitivity of a single-strain-based immunoassay.
Piotr Chomik, Paulina Gil-Kulik, Malgorzata Filas, Agnieszka Wojcieszek, Mateusz Wilinski, Jolanta Karwat, Lidia Kotula, Alicja Niedojadlo, Marcin Czop, Anna Bogucka-Kocka, Maria Cioch, Ryszard Pluta and Janusz Kocki
The BIRC6 gene encodes the Bruce (Apollon) protein. This belongs to the III class of Inhibitors of the Apoptosis Protein (IAP) and demonstrates anti-apoptotic activity (binding, inhibiting and degrading the caspases). Moreover, the Bruce protein shows multilevel activities and additional functions. The Bruce protein is involved in the maintenance of cell viability, and it is also suggested that it plays an important role in cell proliferation and diversification. Many researchers have noticed elevated BIRC6 gene expression in cell lines of brain cancer and ovarian carcinoma, leukemia, breast cancer and even in colorectal cancer tissues. Resistance to chemotherapy-inducted apoptosis in cancers characterized by BIRC6 gene over-expression was also reported. The aim of the study was to assess the BIRC6 gene expression in peripheral blood lymphocytes of patients diagnosed with chronic lymphocytic leukemia.