The histological changes observed in the pituitary corticotrophs, gonadotrophs, adrenocortical tissues and testicular cells in M. vittatus (Bloch, 1794) have been studies during growth, maturation and spawning phases. The studies based on the changes observed in the cell types, shape and size of the cells of the adrenocortical tissues, testes and the overall percentage of gonadotroph (GTH) and thyrotroph (TSH) cells of the pituitary. However, during growth phase, in proximal pars distalis (PPD) the considerable increment of GTH and TSH have been observed having intense aniline blue stain. The corticotrophs (ACTH) also showed significant accumulation of fuchsinophilic cytoplasmic granules. The cytoplasmic features and the architecture of the interrenal cells were well coincident with the increase of different spermatogenic cells. During the maturation phase dense granulation in the GTH and TSH cells appeared to be concomitant with the spermiation. The amount of cytoplasmic granules of the interrenal cells increased than chromaffin cells and was well coincidence with the increase of spermatids and spermatozoa. The hyperactive and vacuolated features of the interrenal cells during spawning phase appeared to be concomitant with the final process of spermiation.
The structural characterization of the olfactory epithelium in Pygocentrus nattereri Kner, 1858 was studied with the help of light as well as scanning and transmission electron microscope. The oval shaped olfactory rosette consisted of 26–28 primary lamellae radiated from midline raphe. The olfactory epithelium of each lamella was well distributed by sensory and non-sensory epithelium. The sensory epithelium contained morphologically distinct ciliated and microvillous receptor cells, supporting cells and basal cells. The non-sensory epithelium was made up of labyrinth cells, mucous cells and stratified epithelial cells. According to TEM investigation elongated rod emerging out from dendrite end of the receptor cells in the free space. The dendrite process of microvillous receptor cells contained microvilli. The supporting cells had lobular nucleus with clearly seen electron dense nucleolus. The apex of the ciliated non-sensory cells was broad and provided with plenty of kinocilia. Basal cells provided with oval nucleus and contained small number of secretory granules. The mucous cells were restricted to the non-sensory areas and the nuclei situated basally and filled with about two-third of the vesicles. The functional significance of various cells lining the olfactory epithelium was discussed with mode of life and living of fish concerned.
An efficient C0 continuous finite element (FE) model is developed based on a combined theory (refine higher order shear deformation theory (RHSDT) and least square error (LSE) method) for the static analysis of a soft core sandwich plate. In this (RHSDT) theory, the in-plane displacement field for the face sheets and the core is obtained by superposing a global cubically varying displacement field on a zig-zag linearly varying displacement field with a different slope in each layer. The transverse displacement assumes to have a quadratic variation within the core and it remains constant in the faces beyond the core. The proposed model satisfies the condition of transverse shear stress continuity at the layer interfaces and the zero transverse shear stress condition at the top and bottom of the sandwich plate. The nodal field variables are chosen in an efficient manner to circumvent the problem of C1 continuity requirement of the transverse displacements. In order to calculate the accurate through thickness transverse stresses variation, the Least Square Error (LSE) method has been used at the post processing stage. The proposed combined model (RHSDT and LSE) is implemented to analyze the laminated composites and sandwich plates. Many new results are also presented which should be useful for future research.