The effect of fertilisation on C and N, P, K nutrients distribution in the Rendzic Leptosol in locality Nitra-Dražovce was studied. We evaluated the following treatments of fertilisation: (1) G (non-fertilised), (2) FYM (farmyard manure – dose 40 t/ha), (3) G+NPK3 (grass + 3rd intensity of fertilisation for vineyards), and (4) G+NPK1 (grass + 1st intensity of fertilisation for vineyards). The soil samples were taken in spring during the years 2008–2015. Obtained results showed that the content of organic carbon (Corg) decreased in the following order: G+NPK1 > FYM > G > G+NPK3 and content of total nitrogen (Nt) decreased in the following order: FYM > G+NPK3 > G+NPK1 > G. The application of NPK in the 1st intensity of fertilisation for vineyards and added FYM build up a Corg at an average rate of 370 and 229 mg/kg/year, respectively. On the other hand, contents of Nt due to fertilisation declined in FYM, G+NPK3 and G+NPK1 at an average rate of 53, 22 and 20 mg/kg/year, respectively. Available P and K contents were also increased after the fertilisation of FYM and NPK. Added fertilisers (G+NPK3) significantly build up a P at an average rate of 10.2 mg/kg/year.