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Csilla Tóthová, Pavol Mudroň and Oskar Nagy

Abstract

The objective of the study was to evaluate the electrophoretic pattern of serum proteins in dairy cows suffering from various inflammatory diseases, and to study the influence of these diseases on the concentrations of protein fractions. Fourty dairy cows with clinical signs of various inflammatory diseases were used in the study, including cows with post-partum metritis (n=10), mastitis (n=7), and hoof diseases (n=23). The cows were of a black pied Holstein-Friesian breed, Slovak spotted breed and their crossbreeds at the age of 3.5 to 8 years. Fourteen clinically healthy dairy cows were taken as the control group. The cows were blood sampled for the determination of total serum proteins and serum protein fractions. The protein fractions were divided into albumin, α1-, α2-, β1-, β2-, and γ-globulins. In cows with post-partum metritis we found significantly lower concentrations of albumin (P<0.001) and significantly higher values of α1-globulins (P<0.01) compared with healthy animals. Significantly higher concentrations of β1-globulins were observed in cows with mastitis (P<0.001), while the γ-globulin fraction was non-significantly higher. In cows with hoof diseases we recorded significantly lower values of albumin (P<0.001), significantly higher concentrations of α1- and β1-globulins (P<0.001), and non-significantly higher β2- and γ-globulins. Moreover, in the electrophoretic pattern of serum proteins we found β-γ bridging in thirteen cows with hoof diseases. Presented data suggest marked influence of inflammatory diseases on the concentrations of serum protein fractions in dairy cows. However, further investigations are needed to establish the diagnostic utility of serum protein electrophoresis in bovine clinical practice in relation to inflammatory diseases.

Open access

Csilla Tóthová, Oskar Nagy, Veronika Nagyová and Gabriel Kováč

Abstract

The objective of this study was to describe the physiological changes in the concentrations of acute phase proteins (APPs) in calves during the first month of life, including pre-, postcolostral and milk feeding period. Seven clinically healthy calves were used in this study. Calves received colostrum and whole milk from their dams. The first blood sampling was performed before colostrum intake (day 0) and then at 1, 2, 7, 14 and 30 days of age. Blood serum was analyzed for the concentrations of haptoglobin (Hp), serum amyloid A (SAA), α1-acid glycoprotein (AGP), ceruloplasmin (Cp), and albumin (Alb). The results showed significant changes in the serum concentrations of Hp, SAA and Cp (P<0.001, P<0.01, P<0.01). Their lowest concentrations were found after birth, and a gradual increase was observed after colostrum intake until day 7 of life. Another trend was observed in the concentrations of albumin with a more marked decrease of values 1 day after colostrum intake and subsequent significant increase of values until the end of the first month of age (P<0.001). Sampling time had no significant effect on the concentrations of AGP. The values observed at birth and on day 1 of life were relatively stable. The concentrations of AGP increased slightly from day 2 until the end of the first month of age. These results suggest that the concentrations of APPs in the neonatal period are influenced by colostrum intake and age. This should be taken into consideration for the precise interpretation of these analytes in young animals.

Open access

ŠOLTÉSOVÁ Helena, NAGY Oskar, TÓTHOVÁ Csilla, PAULÍKOVÁ Iveta and SEIDEL Herbert

Abstract

The purpose of this study was to evaluate in calves the effects of respiratory diseases of various severities on blood gases, acid-base balance and plasma lactate concentration. We included in the study 128 calves with clinical signs of various severities and outcomes of respiratory disorders. The age of the calves ranged from 1 to 12 months. According to the clinical findings, course of the disease and time of blood collection, the sick calves were divided into three groups: RD 1 (n=34) – calves with moderate clinical signs; RD 2 (n=73) – calves with severe respiratory symptoms and RD 3 (n=21) – calves with severe respiratory symptoms and died within 48 hours after blood sampling. Into the study we also included one group of 28 clinically healthy calves. In the arterial blood we evaluated: blood pH, partial pressure of carbon dioxide – pCO2, partial pressure of oxygen – pO2, concentration of actual bicarbonate – HCO3 , base excess – BE, and saturation of hemoglobin by oxygen – O2-sat. The concentration of lactate was determined in the blood plasma. Significant effects of health status and severity of respiratory diseases were recorded in all the evaluated variables. Marked hypoxemia and hypercapnia were found in calves with severe respiratory disorders and in animals that died within 48 hours after blood collection. In most calves, the acid-base changes were characterized as compensated respiratory acidosis. The highest mean lactate values were recorded in calves that died within 48 hours after blood sampling. The presented results suggest the usefulness of the analysis of blood gases and acid-base status in the evaluation and prognosis of various degrees and severity of respiratory diseases in calves, and in monitoring the patient response to treatment. Although plasma lactate analysis is less consistent with the severity of respiratory diseases, it might be helpful in the indication of an untreatable stage of the disease and upcoming death.

Open access

Tomáš Lipták, Valent Ledecký, Marek Ďurej, Oskar Nagy and Mária Kuricová

Abstract

The aim of this study was to investigate the influence of general anesthesia on selected blood parameters in 53 surgical patients belonging to five ASA groups. The venous blood pH during the preoperative period was under physiological values only in the ASA V group of dogs. The lowest average values of pH levels were found in all ASA groups during the 30th minute of the surgical procedure. The pre-operative measurements revealed the average concentration of calcium in the blood serum below the physiological range in the groups with higher anesthetic risk, ASA III, IV and V. Most dogs with hypocalcemia during the whole monitored period were in the ASA III group (69.2%). After premedication and sedation a decrease in the concentration of calcium in all groups was observed, except for the ASA IV group. Changes in the concentration of calcium were significant in the ASA II group (P ≤ 0.01). Between the groups, there were no significant differences reported in calcium concentrations during the monitored period. The lowest average value of phosphorus concentration was recorded in the ASA III group and the highest in the ASA V group. In the postoperative period the increase in phosphorus concentrations was observed in all groups except ASA III. Acidaemia, hypocalcaemia and hyperphosphatemia may present a potential risk mostly in endangered animals, so additional monitoring of these parameters, along with commonly used anesthetic monitoring, is essential and might be significantly helpful.

Open access

Lipták Tomáš, Capík Igor, Ledecký Valent, Nagy Oskar, Kuricová Mária, Tóthová Csilla, Maďari Aladár, Farbáková Jana, Petrovič Vladimír and Horňák Slavomír

Abstract

The aim of this study was to compare the effects of different premedication protocols followed by a propofol/fentanyl TIVA on cardio-respiratory and hemodynamic changes in twenty-four dogs randomly divided into two groups (AMD-group: medetomidine, atropine and diazepam; AXD-group: xylazine, atropine and diazepam). Cardiorespiratory variables, acid-base indices, quality of sedation, induction, intubation and recovery were recorded throughout the experiment. Significant changes were observed for the pO2 level, which was increased in the AMDgroup from 90 min. (*P< 0.05) to 120 min. (**P< 0.01) of anesthesia. This can be explained by a reduction of the administration rate of propofol/fentanyl TIVA and oxygenation initiated due to excessively deep anesthesia detected by an anesthetsiologist, leading to improved ventilation and increased pO2. The pCO2 (*P < 0.05) reached more preferable values during the first 30 min. and pH (**P< 0.01) was significantly improved within the first 60 min. in the AXD-group thanks to less depressant effects of xylazine. Within the first 30 min. of anesthesia a significant heart rate difference between the groups was accompanied with significantly higher BP (hypertension) in the AXD-group (10 min. ***P< 0.001, 30 min. **P< 0.01). This points to the possibility of atropine application only in the case of a tendency to bradycardia followed by hypotension. It can be concluded that xylazine is a better option for the premedication of a propofol/ fentanyl TIVA in dogs undergoing a prolonged surgical intervention, in spite of the fact that lower sedation scores were attained. We have detected significantly less adverse cardio-respiratory and hemodynamic effects of xylazine, and a shorter recovery time when compared to medetomidine