We surveyed antibiotics in drinking water in Austria. Testing for 37 antimicrobials was performed by online solid-phase extraction–liquid chromatography–high-resolution mass spectroscopy method. Selection of sample sites for drinking water resources focused on areas considered susceptible to contamination and on geographic population distribution. Sulfamethoxazole was detected in 10 (5%) of 200 samples collected in 2014. Five samples showed concentrations above the limit of quantification (2.5 ng/l). Concentrations measured were ≤8.9 ng/l, making toxic effects highly unlikely. However, even low-level concentrations of antibiotics could increase bacterial resistance. The presence of antibiotics is presently not regulated. We assume anthropogenic pollution (not animal husbandry) as source and ask for a maximum permissible value of antibiotics in drinking water.