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Jinghong Ma, Yuhong Kang, Ning Ma, Wenming Hao, Yan Wang and Ruifeng Li

Abstract

A high-acidity HUSY zeolite with mesoporous structure was prepared by alumination with a dilute aqueous NaAlO2 solution and characterized by XRD, N2 adsorption, IR framework vibration and 29Si MAS NMR methods. The results indicated the extra-framework aluminum was reinserted into the tetrahedral framework through isomorphic substitution of framework Si (0Al) sites by Al ions, whereas the crystal and micropore structure were unaltered. FTIR spectra of hydroxyl vibrations and pyridine adsorbed on realuminated zeolites showed that the number of Brønsted acid sites and strong Lewis acid sites increased whereas weak Lewis acid sites decreased twice. The mesoporous structure composed of inter-and intra-crystalline pores in the aluminated HUSY increased the external surface area of the zeolite, improving accessibility of molecules to the active sites and enhancing its catalytic ability. The realuminated HUSY zeolite supported with Ru catalyst exhibited a higher catalytic activity for benzene hydrogenation than the parent HUSY zeolite; the reaction rate in comparison to the mesozeolite increased by 5.5 times.

Open access

Zhenyu Wang, Ning Ma, Dehua Chang and Huimin Huang

Abstract

Background: Cell seeding technique is a fundamental component of the tissue engineering construction. Effective cell seeding can not only shorten the construction time but also improve the final outcome of tissue engineered substitutes.

Objective: We improved the seeding efficiency of monolayer cells on the surface of biomaterials in tissue engineering. Cell sheet technology was applied to seed monolayer cell sheet instead of concentrated cell suspension, which was termed cell-sheet seeding.

Methods: Equal quantities of endothelial cells were seeded on the surface of equally sized acellular bovine pericardia by cell-sheet seeding and cell suspension seeding respectively. After culturing cell-seeded pericardia for 24 hours, the effects of these 2 cell seeding methods were evaluated and compared.

Results: As compared to cell suspension seeding, a larger quantity of loading cells, higher tissue-plasminogen activator (t-PA) and prostacyclin (PGI-2) content, and higher homogeneity of cells distribution on the surface of the pericardia was achieved with cell-sheet seeding. Furthermore, cell-sheet seeding did not affect metabolism or increase endothelial cells damage.

Conclusion: Cell-sheet seeding is an effective and time-saving method of seeding monolayer cells on the surface of biomaterials. This novel seeding method is particularly suitable for building tissue engineered substitutes with monolayered cells distribution and 2-dimensional structure.

Open access

Ning Ma, Xin Li, Hong-bin Wang, Li Gao and Jian-hua Xiao

Abstract

Introduction: Tiletamine-xylazine-tramadol (XFM) has few side effects and can provide good sedation and analgesia. Adenosine 5’-monophosphate-activated protein kinase (AMPK) can attenuate trigeminal neuralgia. The study aimed to investigate the effects of XFM and its specific antagonist on AMPK in different regions of the brain. Material and Methods: A model of XFM in the rat was established. A total of 72 Sprague Dawley (SD) rats were randomly divided into three equally sized groups: XFM anaesthesia (M group), antagonist (W group), and XFM with antagonist interactive groups (MW group). Eighteen SD rats were in the control group and were injected intraperitoneally with saline (C group). The rats were sacrificed and the cerebral cortex, cerebellum, hippocampus, thalamus, and brain stem were immediately separated, in order to detect AMPKα mRNA expression by quantitative PCR. Results: XFM was able to increase the mRNA expression of AMPKα1 and AMPKα2 in all brain regions, and the antagonist caused the opposite effect, although the effects of XFM could not be completely reversed in some areas. Conclusion: XFM can influence the expression of AMPK in the central nervous system of the rat, which can provide a reference for the future development of anaesthetics for animals.

Open access

Haoju Wang, Li Ni, Hongjun Yang, Limin Xu, Ning Ma and Honglei Ding

Abstract

In order to evaluate the prevalence of the Mycoplasma mycoides cluster in goats in Chongqing, China, an epidemiological survey in this area was carried out. A total of 68 samples were subjected to bacteria isolation on Hartley’s medium. Four isolates (three from lung tissue and one from nasal discharges) were recovered from the samples and identified as the Mycoplasma species by their morphological and biochemical characteristics. They were further confirmed by PCR using 16S rRNA specific primer pairs and by restriction enzyme analysis. In vitro antimicrobial susceptibility of the isolates indicated that some strains had developed resistance to the antibiotics tested. This is the first report on the isolation, identification, and molecular characterisation of Mycoplasma species isolated from goats in Chongqing. This study also revealed a prevalence of Mycoplasma species infection in goats in this area.

Open access

Ning Ma, Min Liu, Min Zheng, Xi Chen, Hong-yan Lu, Hong-guang Chen, Jun Zheng, Gui-ying Li, Juan Wang, Guo-wu Liu and Ning Wang

Abstract

Objective To estimate the HIV/AIDS epidemic and treatment demand and predict the situation in the next five years with Spectrum.

Methods Using Spectrum (version:3.54) to estimate the number of new HIV infections, number of people living with HIV, need for ART in adults and children, need for PMTCT and cotrimoxazole in Beijing and Hunan Province. Data used in the model including high-risk populations monitoring data and demographic information, was collected from Beijing Center for Disease Control and Prevention, Hunan Center for Disease Control and Prevention and extracted from statistical yearbooks and published literatures.

Results Few new HIV/AIDS were reported in Beijing prior to 1994, however, the number of HIV infections was increasing rapidly from 1995 to 2008, and decreased after that, increased rapidly again after 2012. The number of patients who need antiretroviral treatment was increasing among the group aged between 15-49 years with young patients aged 15-24 years peaked in 2010 and decreased rapidly after that. Few HIV-infected patients were reported before 1997 in Hunan Province, and the number of new infections was increasing rapidly since 1998. The number of patients who need antiretroviral treatment was increasing among the group aged between 15 - 49 years since 2000 with young patients aged 15-24 years in need of antiretroviral therapy increasing continuously.

Conclusions After HIV infection was first founded in Beijing and Hunan Province, there was a slow growth and then a rapid growth of HIV epidemic. According to prediction of Spectrum, the demand for antiretroviral therapy in Beijing would begin to decline since 2011, meanwhile, the HIV epidemic in Hunan Province would enter a rapid growth period with the demand for antiviral therapy continuing to increase. In this study, Spectrum was applied to estimate the HIV epidemic situation and need for treatment in the next five years in Beijing and Hunan Province. It provides the basis for health resource allocation and policy formulation of HIV management.