Georgeta Pavalache, Nicoleta Matei and Antoanela Popescu
The spectrophotometric method for determination of loratadine using tetraiodomercurate has been applied in various pharmaceutical formulations. The results confirmed that recovery value is optimum and the method is valid, thus it can be used in quality control and evaluation of loratadine tablets, oral formulations of mixed composition, oral solutions, etc. The method is easy and simple to apply, does not require complicated equipment and spectrophotometric reading time is reduced, which allows a large number of analyzes in a relatively short time.
The present paper investigates on the spatial and temporal variability of maximum and minimum air-temperatures in Romania and their connection to the European climate variability. The European climate variability is expressed by large scale parameters, which are roughly represented by the geopotential height at 500 hPa (H500) and air temperature at 850 hPa (T850). The Romanian data are represented by the time series at 22 weather stations, evenly distributed over the entire country’s territory. The period that was taken into account was 1961-2010, for the summer and winter seasons. The method of empirical orthogonal functions (EOF) has been used, in order to analyze the connection between the temperature variability in Romania and the same variability at a larger scale, by taking into consideration the atmosphere circulation. The time series associated to the first two EOF patterns of local temperatures and large-scale anomalies were considered with regard to trends and shifts in their mean values. The non- Mann-Kendall and Pettitt parametric tests were used in this respect. The results showed a strong correlation between T850 parameter and minimum and maximum air temperatures in Romania. Also, the ample variance expressed by the first EOF configurations suggests a connection between local and large scale climate variability.
Nicoleta Matei, Antoanela Popescu, Gabriel-Lucian Radu, Victoria Artem and Georgeta Pavalache
The study investigates the pollution with heavy metals of grapes and soil. The grapes nourish from the respective soil, with all existing substances: either nutrients or toxic materials. This link, between grapes and soil, made mandatory to focus on observing the level of toxic materials in both samples grapes and land. The aim of this research is to analyze the level of Cd and Pb in Vitis vinifera L. grape fruits and soil, by flame atomic absorption spectrometry (FAAS) method. The grapes and the soil used in this work were sampled from the Murfatlar City, a nonindustrial area, placed far from the car traffic pollution. Cd and Pb were quantified, after the chemical mineralization of the samples using nitric acid. It can be noticed that the values of cadmium and lead concentrations in grapes were lower than the recommendable maximum limit.
Georgeta Pavalache, Nicoleta Matei, Antoanela Popescu and Verginica Schroder
The aim of research is to assess the active substance by a HPLC method for the separation and quantitative determination of loratadine. The method has been developed and validated on the standard solutions, in previous research. The current study was undertaken to present the results obtained from loratadine determination in biological samples (human serum, urine and breast milk). These results may be applicable on patients with different physiological conditions (aging, pregnancy or recently giving birth, etc.) and pathological conditions which may interfere with the metabolism of loratadine. The used HPLC method detected loratadine concentrations in human serum samples, respectively urine samples, at 2 hours after drug administration. The method detected traces of loratadine which passed into breast milk, as well. Data were statistically interpreted using MED CALC 10.2 software. These results show that the applied method can be used for quantitative analysis of loratadine in biological fluids (all permissible limits of quality specifications being in the range 95- 105%).
Nicoleta Matei, Simona Dobrinas and Gabriel Lucian Radu
The objective of the present work was to adapt the Prussian Blue reaction for the determination of ascorbic acid. The procedure was successfully applied for the determination of ascorbic acid in red and white grapes (Vitis vinifera L.) just previous ingathering. In the present work was used the red and white grapes from Murfatlar vineyard: Mamaia, Cabernet Sauvignon, Merlot, Pinot Noir, Chardonnay, Sauvignon, Muscat Ottonel and Riesling Italian. The results were situated in the range of 0.67 - 1.79 mg vitamin C/100g product for red grapes and respectively 0.50 - 1.49 mg vitamin C/100g for white grapes.
Polyphenolic compounds were determined from a pharmaceutical (tincture) and a cosmetic preparation (rose water), both obtained from the Rosae damascenae flores. Separation of the phenolic compounds was done by a HPLC method, using a Zorbax XDB or equivalent column C18, 250 mm x 4,6 mm; 5 μm. A gradient elution was performed with phosphoric acid and acetonitrile eluted under gradient conditions. The flow rate was 1.5 mL/min and the injection volume was 20 μL. HPLC method for determination of caftaric acid presented in this paper, has been validated. The results were statistically analyzed with SPSS 10 software.
Horatiu Miresan, Cosmin Rosca, Nicoleta Matei, Florentina Roncea, Radu Cazacincu, Irina Iancu, Emil Stefanescu, Daniel Enache, Mihaela Bratu and Antoanela Popescu
A rapid high performance liquid chromatography method, using a monolithic column, was developed for quantitative determinations of benzodiazepines (diazepam, clonazepam, lorazepam, midazolam) in whole blood. A liquid-liquid extraction step with n-chlorobutane isolates the drugs from alkalinized blood. The separation was carried out in reversed phase conditions using a Chromolith Performance (RP-18 100x4.6 mm) column. For the mobile phase, a mixture of a phosphate buffer (pH= 2.5)/acetonitrile (65/35 v/v), in isocratic mode at 2 mL/min. An ultraviolet spectrophotometer was used as the detector at the wavelength of 220 nm. The total run time of the analytical method is less than 4-6 minutes. The calibration curves showed linearity and the correlation coefficient of each individual curve was greater than 0.995. The method was linear over a concentration range of 0.03-0.6 μg/mL for clonazepam, lorazepam and midazolam. For diazepam of linearity was over the range 0.04-5.0μg/mL. Quantification limits ranged from 0.03-0.04μg/mL and the accuracy were from 80% to 105% for the recovery test.
The results indicate that this analytical method is simple, specific, accuracy, sensitive, demonstrating from the validation data and a higher robustness. The proposed method is applied routinely in forensic toxicological analysis involving blood.