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  • Author: Natalia Styś-Fijoł x
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Open access

Natalia Styś-Fijoł, Wojciech Kozdruń and Hanna Czekaj

Abstract

Introduction: The purpose of this study was to determine the occurrence of avian reovirus (ARV) infections in wild birds in Poland and attempt to propagate the selected ARV strains in chicken embryo kidney (CEK) cells or chicken SPF embryos. Material and Methods: The study included 192 wild birds representing 32 species, collected between 2014 and 2016. A part of the S4 segment encoding the σNS protein of avian reoviruses (ARVs) isolated from different species of wild birds from that period was amplified. Results: The presence of ARV was demonstrated in 58 (30.2%) wild birds belonging to nine orders. The isolated strains were propagated in chicken embryos by yolk sac inoculation, and CPE was induced in the infected CEK monolayer. Agar gel precipitation showed that two ARV isolates from rock pigeon and mute swan shared a common groupspecific antigen with chicken reovirus S1133. Specific products of predicted size were found in two ARV isolates from the chicken embryo passage and 13 ARVs isolated from CEK cells. Conclusion: The study indicates the high prevalence of ARV among wild birds in Poland and its possible transmission to farmed birds.

Open access

Natalia Styś-Fijoł, Wojciech Kozdruń and Hanna Czekaj

Abstract

Introduction: The aim of the study was to investigate the occurrence of goose haemorrhagic polyomavirus (GHPV) in wild birds inhabiting Poland.

Material and Methods: Samples from 508 birds of different species were obtained between 2010 and 2015. The internal organ sections were homogenised and then total cellular DNA was isolated. The study was performed by means of PCR assay using primers complementary to the VP1 gene of the GHPV.

Results: The presence of genetic material of GHPV was detected in 22 (4.33%) samples.

Conclusion: It was the first such study in Poland to emphasise the role of wild birds as a potential source of GHPV infection for farmed geese.

Open access

Hanna Czekaj, Wojciech Kozdruń, Natalia Styś-Fijoł, Jowita Samanta Niczyporuk and Karolina Piekarska

Abstract

Introduction

Avian reovirus (ARV) infections in poultry populations are reported worldwide. The reovirus belongs to the genus Orthoreovirus, family Reoviridae. The aim of the study was to evaluate the incidence of ARV infections in the poultry population based on diagnostic tests performed in 2010–2017.

Material and Methods

Samples of the liver and spleen were collected from sick birds suspected of ARV infection and sent for diagnostics. Isolation was performed in 5–7-day-old SPF chicken embryos infected into the yolk sac with homogenates of internal organs of sick birds. Four primer pairs were used to detect the σNS, σC, σA, and μA ARV RNA gene fragments. A nested PCR was used for the detection of the σNS and σC genes.

Results

In 2010–2017, ARV infection was found in birds from 81 flocks of broiler chickens and/or layers, 8 flocks of slaughter turkeys, and in 4 hatchery embryos at 17–20 days of incubation. The primers used in RT-PCR and nested PCR did not allow effective detection of ARV RNA in all virus-positive samples.

Conclusion

The problem of ARV infections in the poultry population in Poland still persist. The primers used for various ARV segments in RT-PCR and nested PCR did not allow effective detection of RNA in the visceral organs of sick birds. The presented results confirm the necessity of using classical diagnostic methods (isolation in chicken embryos, AGID).

Open access

Wojciech Kozdruń, Natalia Styś-Fijoł, Hanna Czekaj, Piotr Indykiewicz, Rafał Sandecki and Jowita Samanta Niczyporuk

Abstract

Introduction

Avian poxvirus infections are widespread in the domestic poultry population but are also reported in wild birds. In poultry, these infections cause significant economic losses, while wild birds may be a reservoir for poxvirus which affects breeding poultry. However, wild birds may also exhibit characteristic anatomopathological changes. This study concerns the infection of wild-living great tits (Parus major) with the avian poxvirus in Poland.

Material and Methods

Samples of internal organs and skin collected from great tits were homogenised and total cellular DNA was isolated. In PCR, the primers complementary to gene encoding the core protein 4b of the HP44 strain of fowl poxvirus (FPV) were used.

Results

After electrophoresis in 2% agarose gel, the PCR product of 578 bp characteristic for FPV was obtained in DNA samples isolated from skin lesions and the heart. The analysis of the nucleotide sequence of the virus strain showed 99% similarity to many poxviruses previously isolated from great tits and other free birds at various sites in the world.

Conclusions

This paper is the first clinically documented evidence obtained in laboratory conditions of avian poxvirus cases in great tits in Poland.