Jakub Sawicki, Vítězslav Plášek and Monika Szczecińska
Recent data on occurrence the sporophytes in populations of dioecious moss, Nyholmiella obtusifolia in North-Eastern Poland
Moss Nyholmiella obtusifolia is relatively common in Poland, but mostly sterile specimens are found in herbarium collections. Sporogenes were created very rare. During bryological explorations conducted in NE Poland in 2009 - 2011, sporophytes were found in four populations of N. obtusifolia, which account for approximately 7% of the surveyed populations.
Zbigniew Celka, Monika Szczecińska and Jakub Sawicki
Genetic relationships between some of Malva species as determined with ISSR and ISJ markers
Two categories of DNA markers were used to determine genetic relationships among eight Malva taxa. A maximum parsimony analysis validated the division of the genus Malva into the sections Bismalva and Malva. The species classified into those sections formed separate clusters. M. moschata was a distinctive species in the section Bismalva, as confirmed by previous genetic research based on ITS and cpDNA sequence analyses. The applied markers revealed a very high level of genetic identity between M. alcea and M. excisa and enabled molecular identification of M. alcea var. fastigiata. Speciesspecific markers were determined for the majority of the analyzed species, permitting their molecular identification. A specific marker supporting the differentiation of M. alcea and M. excisa was not found.
Katarzyna Wąsowicz, Monika Szczecińska and Jakub Sawicki
The reasons for low intrapopulation genetic variation in Lamium incisum Willd
The paper presents results of a study which aim was preliminary screening of intrapopulation genetic variability in Lamium incisum Willd. This weed species is rarely distributed in Poland and lessening its count during the last years. As a plant inhabiting anthropogenic sites it is exposed to extreme conditions and disturbances caused mostly by the progressive intensification of agriculture. In order to investigate the genetic variability of the selected population markers of ISSR category were used. The analysis of chosen individuals with use of three ISSR primers revealed total of 49 loci, of which only 15 were polymorphic. Nei's gene diversity index (HE=0.099) and the mean number of alleles per locus (AE=1.160) indicated low genetic diversity within the examined population. The research presented in this paper allows for a better learning of the genetic variability of the investigated species and considers probable factors influencing its level.
Katarzyna Buczkowska, Jakub Sawicki, Monika Szczecińska, Stanisław Rosadziński, Mariola Rabska and Alina Bączkiewicz
Two morphologically distinct groups of the Calypogeia fissa complex were found in Europe
Two genetically distinct groups (PS and PB) detected previously within the C. fissa complex in Europe were studied with respect to 47 morphometric characters. The two examined groups differed statistically significantly with respect to 34 morphological traits. The forward stepwise method of discriminant analysis showed that the set of diagnostic characters could be limited to nine. The best diagnostic features were morphological characters describing the shape of leaf: length and width of leaf, height of dorsal part and distance from the apex to the ventral base of the leaf, length of the 3rd coordinate of the leaf, and underleaf width as well as characters of the stem: length of internodes and size of internode cells. Plants of the PS group were smaller (shoot width range from 922-1780 μm) than plants of the PB group (1600-3900 μm). Based on genetically identified samples, classification functions for each group were computed and the derived functions were used for the classification of samples from the herbarium collections. The principal component analysis and dendrogram constructed on the basis of Euclidean distance, using the set of diagnostic characters, divided the examined samples into two groups that correlated with groups detected by isozyme markers. Results of multivariable analysis showed that it is possible to satisfactorily characterise morphologically both genetically distinct groups of the C. fissa complex.
Angelika Maria Gomolińska, Monika Szczecińska, Jakub Sawicki, Katarzyna Krawczyk and Piotr Szkudlarz
The rpo genes are characterized by rapidly-evolving sequences. They encode subunits of plastid-encoded (PEP) polymerase (rpoA, rpoB, rpoC1 and rpoC2). This polymerase is one of the most important enzymes in the chloroplasts. The primary aim of the research was to study the rate of molecular evolution in the rpo genes and to estimate these genes as phylogenetic markers based on the example of the genus Erica (Ericaceae). The tested rpo genes demonstrated similarities on multiple levels, for example: phylogenetic informativeness, variation level, intragenic mutation rates and the effect of intragenic mutations on the properties of encoded peptides. This study did not confirm that the analyzed rpo genes are reliable markers and may be helpful in understanding phylogenetic relationships between species that belong to the same genus. The rpoC2 gene was found to be a most useful phylogenetic marker in the Erica genus, while rpoC1 was found to be the least promising gene.
Halina Bednarek-Ochyra, Jakub Sawicki, Ryszard Ochyra, Monika Szczecińska and Vítězslav Plášek
Based on available molecular and morphological evidence, the genus Codriophorus P.Beauv. (Grimmiaceae subfam. Racomitrioideae) proved to be a polyphyletic taxon. It consists of two distinct genera which correspond to two sections of Codriophorus, namely sect. Codriophorus and sect. Fascicularia (Bednarek-Ochyra) Bednarek-Ochyra & Ochyra. The latter section is raised to generic rank as Dilutineuron Bednarek-Ochyra, Sawicki, Ochyra, Szczecińska & Plášek. The new genus consists of the following five species: D. fasciculare (Hedw.) Bednarek-Ochyra, Sawicki, Ochyra, Szczecińska & Plášek, comb. nov., D. brevisetum (Lindb.) Bednarek-Ochyra, Sawicki, Ochyra, Szczecińska & Plášek, comb. nov., D. anomodontoides (Cardot) Bednarek-Ochyra, Sawicki, Ochyra, Szczecińska & Plášek, comb. nov., D. corrugatum (Bednarek-Ochyra) Bednarek-Ochyra, Sawicki, Ochyra, Szczecińska & Plášek, comb. nov. and D. laevigatum (Mitt.) Bednarek-Ochyra, Sawicki, Ochyra, Szczecińska & Plášek, comb. nov.
Vítězslav Plášek, Jakub Sawicki, Ryszard Ochyra, Monika Szczecińska and Tomasz Kulik
The traditionally conceived genera Orthotrichum Hedw. and Ulota F.Weber are here reclassified into six genera, Orthotrichum, Dorcadion Lindb., Nyholmiella Holmen & E.Warncke, Pulvigera Plášek, Sawicki & Ochyra, Plenogemma Plášek, Sawicki & Ochyra, and Ulota, based on morphological differences and partially on molecular evidence. The genus Pulvigera includes P. lyellii (Hook. & Taylor) Plášek, Sawicki & Ochyra (Orthotrichum lyellii Hook. & Taylor) which was selected as its generitype. The genus Plenogemma includes P. phyllantha (Brid.) Plášek, Sawicki & Ochyra (Ulota phyllantha Brid.) which was selected as its generitype.