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Open access

Jolanta Domańska, Tadeusz Filipek and Monika Kwiecień

Streszczenie

Badania przeprowadzono w oparciu o wyniki uzyskane z eksperymentu polowego. Celem badań było określenie wpływu oczyszczonych ścieków miejskich, zastosowanych w dawce pojedynczej, optymalnej dla danej rośliny (ustalona w oparciu o ładunek N i wody) i podwójnej dawce ścieków na zawartość i specjację Cd i Fb w glebie organicznej obojętnej. W pobranych z każdego obiektu, uśrednionych próbach glebowych, oznaczono zawartość ogółem Cd i Fb oraz specjację tych metali. Wyodrębniono 5 frakcji Cd i Pb zdefiniowanych operacyjnie: wymienną (FI), węglanową określaną jako „związaną specyficznie” (F2), związaną z tlenkami Fe-Mn (F3), organiczną(F4) i pozostałości (F5). Stwierdzono, że całkow ita zawartość ołowiu w badanej glebie odpowia- dała wartościom naturalnym, zaś ilość kadmu przekraczała dopuszczalne normy ustalone dla gleb organicznych. Wyniki chemiczne- go frakcjonow ania Cd i Fb w glebie wytworzonej z torfu niskiego, zarówno z obiektów' naw ożonych oczyszczonymi ściekami, jak i z obiektu kontrolnego przedstaw iają następujące uszeregow anie procentowy ch zawartości poszczególnych frakcji w kolejności ma- lejącej: F3>F2>F4>F1>F5 - kadm, F3>F4>F2>F5>F1 - ołów. Oczyszczone ścieki miejskie, będące źródłem łatwo rozpuszczalnych związków organicznych, mogą w'pływać nawiązanie Cd i Fb we frakcji organicznej, co potwierdza odnotowany w badaniach wzrost ilości metali w tej frakcji w glebie.

Open access

Józef Szpikowski, Grażyna Szpikowska and Monika Domańska

Abstract

Meliorations and their specific forms – irrigations – are one of the forms of anthropopressure within geoecosystems. The research studies conducted within the Parsęta basin focused on the sub-irrigation (seepage irrigation) systems formed in the 19th century. Query of archive materials and maps, hydrochemical and phytosociological terrain mapping and laboratory testing of water samples collected were used in the studies. In the study area were found the remains of the thirty old irrigation systems, which together occupy 2% of the Parsęta basin area. For many years most of them have not fulfilled their primary economic functions. Still, these systems have an impact on the cycle of waters and have become an important factor in increasing the geo- and biodiversity within the postglacial landscape. By expanding a range of wetland riparian areas, they fulfil relevant functions to protect surface waters against the supply of biogenic components. Some of them could be used to enlarge wetlands and floodplains within river valleys.

Open access

Joanna Sajewicz-Krukowska, Monika Olszewska-Tomczyk and Katarzyna Domańska-Blicharz

Abstract

Introduction: Due to their immunostimulatory properties TLR ligands are used prophylactically to protect against a variety of viral and bacterial pathogens in mammals. Knowledge of the molecular and functional aspects of TLRs is essential for a better understanding of the immune system and resistance to diseases in birds. For that reason, this study attempted to determine the impact of TLR21 stimulation by its synthetic ligand (CpG ODN, class B) on the chicken immune system.

Material and Methods: Sixty embryonated chicken eggs were randomly allocated into three groups (control and two experimental groups). On day 18 of embryonic development, chickens in one experimental group were administered in ovo a low dose of CpG ODN and the birds of the second experimental group were given a high dose of the ligand. Spleens were collected at 1, 2, 5, and 10 days post-hatching (dph) for analysis of IFN-α, IFN-β, IFN-γ, IL-6, and IL-10 expression using qRT-PCR.

Results: Significant differences were observed in mRNA expression levels of all the measured cytokines associated with the modulation and regulation of the immune response at different time points.

Conclusion: The obtained data clearly demonstrate that immune response induction takes place after in ovo administration of class B CpG ODN, and that the ligand has the ability to induce cytokine responses in neonatal chicken spleen.

Open access

Ewa Domańska-Glonek, Karolina Załuska, Monika Oberc, Ewa Lewicka, Kamil Torres, Anna Torres and Ryszard Maciejewski

Abstract

Introduction. In recent times, patient outcome measurement has developed from being narrowly focussed upon levels of symptomatology and service use, to being a broader assessment of the impact of illness and treatment on the individual. Thus, it can be said that quality of life has become as significant as life expectance. This has brought about a transition in the assessment of treatment. Quality of life (QOL) is a multidimensional concept that usually includes subjective evaluations of both positive and negative aspects of life as it is being led. With regard to healthcare, a cross-sectional comparison of palliative care needs is crucial in understanding differences in the patients' quality of life. Hence, an analysis of programme implementation within different types of healthcare institutions is significant in evaluating current medical care standards. Our study analyzed the satisfaction level and quality of life of patients with Chronic Obstructive Pulmonary Disease (COPD), and after myocardial infarction (MI). Different types of healthcare institutions were evaluated.

Aim. To evaluate patients' satisfaction and quality of life in selected healthcare institutions in southern-eastern Poland.

Material and methods. The quality of life of patients with Chronic Obstructive Pulmonary Disease (COPD) was analyzed through the medical documentation obtained from different types of healthcare institutions. Among these are the pulmonary outpatient department in Moczary, as well as a GP Practice and a Nursing Home in this location. The quality of life of patients after myocardial infraction was analyzed through a survey study conducted at “Polonia” hospital spa in Rymanów Zdrój (the cardiology department).

Results and conclusions. The post-myocardial infarction incident patients had began to care more about their health condition and the quality of life they led. Moreover, their satisfaction level from received treatment and medical care indicated that the cardiological services implemented in southern-eastern Poland has proceeded in a good direction. Of note, these patients were systematically under specialist control. In contrast, among patients with COPD, only those under everyday care in the Nursing Home in Moczary received a similar systematic treatment, thus, COPD patients in Moczary lead a poor quality of life. This indicates a need to re-evaluate the current programmes and services provided by health care institutions in this region.

Open access

Michał Jóźwiak, Krzysztof Wyrostek, Katarzyna Domańska-Blicharz, Monika Olszewska-Tomczyk, Krzysztof Śmietanka and Zenon Minta

Abstract

Introduction: The aim of the study was to test the utility of Flinders Technology Associates filter paper (FTA® Cards) for molecular detection and storage of avian influenza virus (AIV). Material and Methods: There were two strains of AIV used in the study: low pathogenicity H7N1 and high pathogenicity H5N1 subtypes. Detection of viral material was conducted using molecular RT-PCR and rRT- PCR method. Results: The infectivity of LPAIV/H7N1 and HPAIV/H5N1 was completely inactivated within 1 h and 24 h after adsorption to FTA® Cards at room temperature, respectively. Viruses stored on FTA® Cards had detection limit approximately 1 log10 lower than live viruses. Viral RNA of both strains were detectable on the cards by rRT-PCR for a minimum of 150 d, irrespectively of storage temperatures (room temperature, -20ºC). RNA was also detected in all samples obtained from SPF chickens experimentally infected with HPAI/H5N1 on 3rd and 4th day post-infection (p.i.).

Conclusion: FTA® Cards enable safe and effective alternative transport of samples for molecular diagnosis of AIV.

Open access

Ewelina Lulińska-Kuklik, Masouda Rahim, Daria Domańska-Senderowska, Krzysztof Ficek, Monika Michałowska-Sawczyn, Waldemar Moska, Mariusz Kaczmarczyk, Michał Brzeziański, Ewa Brzeziańska-Lasota, Paweł Cięszczyk and Alison V. September

Abstract

Collagen alpha-1(V) chain, encoded by the COL5A1 gene, plays a crucial role in abundant fibrillar collagens supporting many tissues in the body containing type I collagen and appears to regulate the association between heterotypic fibers composed of both type I and type V collagen occurring among others in muscles, tendons and ligaments. Taking this fact into consideration we decided to examine the association between COL5A1 rs12722 and rs13946 polymorphisms, individually and as inferred haplotypes, with anterior cruciate ligament rupture risk (ACLR) in professional soccer players. A total of 134 male professional soccer players with surgically diagnosed primary anterior cruciate ligament ruptures and 211 apparently healthy male professional soccer players, who were without any self-reported history of ligament or tendon injury, were included in the study. Both the cases and the healthy controls were recruited from the same soccer teams, of a similar age category, and had a comparable level of exposure to anterior cruciate ligament injury. Genomic DNA was extracted from oral epithelial cells using GenElute Mammalian Genomic DNA MiniprepKit. All samples were genotyped for the rs12722 and rs13946 polymorphisms using a Rotor-Gene realtime polymerase chain reaction. Statistically significant differences in the genotype frequencies for the COL5A1 rs13946 polymorphisms in dominant modes of inheritance occurred (p = 0.039). Statistically significant differences were documented only in the dominant model under the representation tendency of the C-C haplotype in the ACLR group compared to controls (p = 0.038). Our results suggest that variation in the COL5A1 gene may be one of the non-modifiable factors associated with the ACL injury in professional soccer players. The C-C rs12722-rs13946 haplotype provides a protective effect against the ACL tear.