There is an increasing focus on bridging human behaviour and attitudes towards sustainability. This article focuses on the factors that influence sustainable behaviour of working people. Based on a systematic and comparative analysis of scientific literature, the authors of the paper present the theoretical conceptual model, which illustrates sustainable behaviour. The aim of the empirical research is to examine how employees relate to sustainable behaviour across generations, genders and different modes of education through economic, environmental and social domains. A quantitative method in the form of a survey was selected to capture individual employee attitudes and actions regarding sustainable behaviour. A total of 412 complete responses from Lithuanian employees were used for data analysis. The results of empirical research revealed a significant relationship between gender, generation and education, and sustainable employee behaviour.