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  • Author: Mihaela Cristiana Popp x
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The Presence of Iron and Iucc Virulence-Associated Genes in Romanian Apec Isolates

Abstract

Colibacillosis in poultry is relating with the colonisation with so called Avian Pathogen E. coli (APEC) strains. It is already known that usually in the APEC isolates are present at least 13 virulence-associated genes. We selected 12 non-repetitive E. coli isolates from different Romanian poultry outbreaks. Isolates have been evaluated for the presence of the virulence-associated genes, iroN and iucC. The DNA extraction was made using QIAamp cador Pathogen Mini Kit (Qiagen). The amplification protocol was: a cycle of denaturation at 94°C for 5 min followed by 35 cycles of 94°C for 30 s, 63°C for 45 s and 72°C for 105 s, and a cycle of 72°C for 7 min. Both virulence-associated genes were identified in 83.33% (11/12) isolates. In two APEC isolates, only one gene was identified, iroN or iucC, respectively. According to these preliminary results it could be assumed that iroN and iucC genes are independently expressing their virulence.

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Multiple Intestinal Lymphoma

Abstract

Gastrointestinal tract is the most common location for extralymphonodular lymphomas. The small intestine is affected only in 9% of the cases. Intestinal lymphoma may have single or multiple location. This paper describes a case of multiple location in the small intestine of a non-Hodgkin B-cell in a 53 years old patient, who was initially diagnosed with bilateral pneumonia with pleurisy with E. coli, steeper on the right side, but the persistence of symptoms as fever, malaise, despite appropriate treatment, required further investigation. The CT exam observed fluid collection in the hypogastrium around a digestive loop. The patient underwent surgery, the intraoperative foundings being: a large mesenteric tumor ~ 5 cm in diameter, a terminal ileal mesenteric tumor, a mesenteric tumor ~ 6 cm in diameter, omentum with nodular formations, a tumor ~ 3.3/2.5.1 cm in the abdominal wall, pseudotumoral appendix. Segmental enterectomy with entero-enterostomy, excision of mesenteric tumors, appendectomy and omentectomy were performed. Pathological diagnosis was non-Hodgkin marginal zone B-cell MALT type lymphoma of the small intestine with extension to the appendix, meso, omentum and abdominal wall. Postoperatively, the patient received chemotherapy for remission.

Open access