During the years 2010-2012, we observed the spatial activity of long-eared owls by the radio telemetry in an agricultural land. The average home range size of tracked long-eared owls for 100 and 95% minimum convex polygon (MCP) was 415.93 and 350 ha, respectively. Between the breeding and the non-breeding season, we did not record significant differences in the size of home ranges. Open land units (meadows and arable lands) belonged to the most abundant land units in the home ranges of tracked owls (mean for 100 and 95% MCP was 24.6 and 24.3%, respectively). Forest edges with their ecotone character also represented the abundant land unit (mean for 100 and 95% MCP was 11.4 and 10.6%, respectively). An amount of built-inhabited areas in home ranges (mean for 100 and 95% MCP was 8.2 and 10.1%, respectively) correlated positively with their size (Spearman rank correlation: for 100% MCP: rs = 0.83, p <0.05; for 95% MCP: rs = 0.91, p <0.05) that indicates long-eared owls to be avoiding built-inhabited areas as an area of the food getting. Two individuals of long-eared owl changed the winter roosts during one non-breeding season, which were at a distance of 650 m from each other.