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Masoud Sabouri Ghannad, Soroor Afagh Hojati, Mohammad Mirzaei and Amirhossein Sahebkar

Abstract

Backgroud: Hepatitis B virus (HBV) and Hepatitis C virus (HCV) are the main causes of liver related morbidity and mortality.

Objective: To assess the epidemiologic features of HBV and HCV infections between 2004 and 2007 in the Hamadan province, Iran.

Methods: In a cross-sectional study, epidemiologic and demographic characteristics of patients infected with HBV or HCV, or both, during about four years were collected from health centers in the Hamadan province.

Results: Overall, there were 1533 infected patients (66.2% with HBV, 32.5% with HCV, and 1.3% with both). Male patients comprised 74.4% of patients. In view of marital status, 71.3% were married, 27% single, and 1.7% were widowed or divorced. The majority of patients (78.4%) inhabited urban areas while 21.6% inhabited rural areas. Patients <4 years old had the lowest rate of infection (0.26%), while the highest incidence (13.11%) was found in the 25-29-year-old age range. With respect to serologic markers, 61.4%, 33.7%, 4.4% and 0.3% of patients were HBsAg+, HCVAb+, HBsAb+ and HBeAg+, respectively. Overall, the incidence of HBV was found to be decreased from 2004 to 2007, while the incidence of HCV increased from 2004 to 2007.

Conclusion: The prevalence of HBV infection decreased during the period of study. This could be attributed to the increasing public vaccination of neonates and people at high-risk, which began about 15 years ago. However, it seems that an increasing incidence of high-risk behaviors including intravenous drug abuse with shared syringes has led to an increased incidence of HCV infection.

Open access

Mehrangiz Khajeh Karamoddini, Seyed Ahmad Emami, Masoud Sabouri Ghannad, Esmaeel Alizadeh Sani and Amirhossein Sahebkar

Abstract

Background: Drug resistance to current anti-herpetic drugs has been increasingly reported. Therefore, there is a need for finding new antiviral agents, in particular from natural sources.

Objective: In the present study, antiviral activity of subset extracts obtained from aerial parts of Artemisia including A. incana, A. chamaemelifolia, A. campesteris, A. fragrans, A. annua, A. vulgaris, and A. persica were investigated against Herpes Simplex type I (HSV1).

Methods: Different concentrations of extracts (400, 200, 100, 50, 25, 12.5, 6.25, and 3.125 μg/mL) were obtained from subset of each plant separately, and used against KOS strain of HSV1 in HeLa cells. After 24 hours incubation, tetrazolium dye (MTT), was added. The dye absorption by viable cells was measured and compared to the positive control (extract-untreated cells) and acyclovir (as anti-viral agent).

Results: The extracts obtained from A. annua had the highest antiviral activity while those of A. chamaemelifolia showed the lowest activity.

Conclusion: Subset extracts of A. annua may be an appropriate candidate for further development of anti HSV1 infection.

Open access

Aliasghar Bahari, Masoud Sabouri Ghannad, Omid Dezfoulian, Fereydon Rezazadeh and Ali Sadeghi-Nasab

Abstract

Introduction: The aim of this study was to use TaqMan real-time PCR technique to investigate Jaagsiekte sheep retrovirus (JSRV) proviral DNA in whole blood samples of sheep, and compare the results to those of histopathological examinations. Material and Methods: Eighty blood samples from clinically healthy sheep were randomly collected before the animals were slaughtered. Ten tissue samples from each lung and associated caudal mediastinal lymph node were taken. Results: Fifteen (18.75%) blood samples were found to contain proviral DNA, and 11 (13.75%) corresponding lung samples showed microscopic changes consistent with ovine pulmonary adenocarcinoma. None of the samples displayed metastases to the caudal mediastinal lymph nodes. The prominent pattern of neoplastic nodules consisted of acinar (alveolar) form. Conclusion: The results indicated the higher sensitivity of real-time PCR compared to histopathological examinations in detection of ovine pulmonary adenocarcinoma.