Search Results

You are looking at 1 - 9 of 9 items for

  • Author: Marija Ilić x
Clear All Modify Search
Open access

Marija Ilić, Ivan Kovačević and Jelena Parojčić

Abstract

With the increased reliance on in vitro dissolution testing as an indicator of in vivo drug behavior and the trend towards the in silico modeling of dosage form performance, the need for bioperformance dissolution methodology development has been enhanced. Determination of the in vivo drug delivery profile is essential for the bioperformance dissolution test development and in vitro/in vivo correlation modeling, as well as the understanding of absorption mechanisms. The aim of this study was to compare different methods in terms of their usefulness and applicability in deciphering in vivo delivery of nifedipine administered in modified release dosage forms. A detailed survey of publications on nifedipine pharmacokinetics was done and used to identify the magnitude of food effect. In vitro dissolution testing was performed under various experimental conditions. Obtained results indicate the potential for using the developed in silico model coupled with discriminative in vitro dissolution data for identification of the in vivo drug product behavior

Open access

Biljana Vučković, Mirjana Đerić, Tatjana Ilić, Višnja Čanak and Marija Žarkov

Decrease of Fibrinolytic Potential in the Occurence of Cerebral Ischemia

One of the most present clinical manifestations of long and progressive atherothrombotic occurrences is the ischemic cerebrovascular insult, one of the leading causes of death and illness in the world. Lately, a growing number of scientists believe that disorders in the fibrinolytic mechanism function are the key to the occurrence of cerebral ischemia. The goal of this study is to investigate whether the disorder of the fibrinolytic mechanism has influence on the occurrence of ischemic cerebrovascular insult. Our study includes 90 examinees, 60 of which suffer from ischemic cerebrovascular insult and 30 are clinically healthy examinees forming the control group. The results of our investigation show that statistically a significantly larger number of patients has decreased fibrinolytic potential comparing with controls (p < 0.01). According to this, it has been noted that euglobulin lysis clot time in the patient group is significantly longer (p = 0.005). Statistically, no significant difference has been noted related to the activity of plasminogen (p = 0.085). Further on, the plasminogen activator inhibitor-1 values among the patients have been significantly higher (p = 6.20 x 10-11). Moreover, significantly higher values of tissue-type plasminogen activator antigen have been statistically noted in the patient group (p = 5.20 x 10-5). The results of this investigation impose the conclusions that the decrease in fibrinolytic potential affects the occurrence of ischemic cerebrovascular insult, that it is directly connected to the higher levels of plasminogen activator inhibitor-1 and that the growth of tissue-type plasminogen activator antigen concentration participates in the decrease of fibrinolytic potential among patients suffering from cerebral ischemia.

Open access

Jelena Krizmanić, Marija Ilić, Danijela Vidaković, Gordana Subakov-Simić, Jelena Petrović and Katarina Cvetanović

Abstract

Diatom samples were collected during July 2010 at 15 localities from different types of substrate (stone surfaces, sand, mud, filamentous algae and submerged mosses) from the Dojkinci River. During the research period, 124 taxa were determined within 43 genera. Among numerous common diatoms we recorded three taxa for the first time in Serbia: Brachysira intermedia (Øst.) Lange-Bertalot, Chamaepinnularia mediocris (Krass.) Lange-Bertalot and Navicula tridentula Krass. Also, we observed 21 taxa which are rarely recorded taxa for Serbia. The most interesting was Diatomella balfouriana Grevill. that was previously known only from the River Tisa near Titel. In the studied material, it was identified only in samples collected from the surface of boulders with mosses at the third locality. Their morphology, distribution and ecology are presented in this paper.

Open access

Milica Veljković, Sonja Ilić, Nenad Stojiljković, Ljubinka Velicković, Dragana Pavlović, Mirjana Radenković, Suzana Branković, Dusanka Kitić and Marija Gocmanac Ignjatović

Summary

The aim of this study was to investigate whether green tea extract has beneficial effect on gentamicin-induced acute renal failure. The investigation was conducted on thirty-two Wistar rats divided into four groups of 8 animals each. Control (C) group received normal saline. GT group received green tea extract orally, 300mg/kg. GM group received gentamicin intraperitoneally, 100mg/kg and GT+GM group received both gentamicin and green tea extract.

Histological sections of kidney in GM group revealed necrosis of proximal tubules, vacuolization of cytoplasm and massive mononuclear inflammatory infiltrates in interstitium. Coadministration of green tea with gentamicin had renoprotective effect and showed only mild infiltrations, normal glomeruli and alleviated tubular degeneration. Analysis of biochemical parameters showed significantly higher urea and creatinine serum concentrations in GM group in comparison with C group and GT+GM group (p<0.001). Plasma lipid peroxidation biomarker MDA was significantly higher in GM group than those in C group (p<0.001), whereas the values for GT+GM group were significantly lower than MDA recorded for GM group (p<0.001).

Beneficial effects of green tea on gentamicin-induced nephrotoxicity is explained through decrease of oxidative stress and lipid peroxidation.Our results indicate that green tea administration has nephroprotective effect on oxidative stress and acute renal failure caused by gentamicin.

Open access

Marija Stojanović, Ljiljana Šćepanović, Dušan Mitrović, Vuk Šćepanović, Radomir Šćepanović, Marko Djuric, Slobodan Ilić, Teja Šćepanović and Dragan Djuric

Abstract

Recent studies have confirmed that hyperhomocysteinemia is associated with gastrointestinal diseases; however, the direct effect of homocysteine on gastrointestinal reactivity still remains unknown. The aim of this study was to demonstrate how homocysteine may affect nitric oxide mediated duodenal relaxation and whether cholinergic receptors and K+ channels take part in stimulating motility, as well as to explore whether oxidative stress is associated with homocysteine-mediated effects. Experiments were carried out on male rats, body mass 250-300 g. Two groups of animals were treated by i.p. application of saline and D,L-Hcy (0.6 μmol/g bm). After 2h of incubation, the duodenal segments were prepared for biochemical analysis and contractile response measurements in an organ bath with Tyrode’s solution. Effects of TEA (10 mmol/L) and L-NAME (30 μmol/L) on duodenal contractility in the presence of D,L-Hcy (0.6 μmol/g bm) were investigated. Elevated homocysteine levels seem to be of crucial importance for the deterioration of contractility through nitric oxide mediated relaxation, and, in part, by activation of K+ channels. Hcy showed direct promuscarinic effects, since 30 min pretreatment of rat duodenum significantly enhanced the contractile effect of increasing concentrations of ACh (10−9-10−2 mol/L). Catalase activity, superoxide dismutase, glutathione peroxidase and the total antioxidant system were reduced while the thiobarbituric acid-reactive substances level was elevated. Our data showed a consistent profile of gastrointestinal injury elicited by sulfur-containing amino acid-homocysteine. This could contribute to explain, at least in part, the mechanisms involved in human gastrointestinal diseases associated to hyperhomocysteinemia.

Open access

Milena Jurisevic, Gordana Radosavljevic, Aleksandar Arsenijevic, Marija Milovanovic, Nevena Gajovic, Dragana Djordjevic, Jelena Milovanovic, Bojana Stojanovic, Aleksandar Ilic, Tibor Sabo and Tatjana Kanjevac

Abstract

The design of platinum based drugs is not a new field of interest. Platinum complexes are widely used as anticancer agents and currently, approximately 30 platinum(II) and platinum(IV) entered into some of the phases of clinical trials. A special place in today’s research belongs to platinum complexes with diammine ligands. A large number of edda (ethylenediamine- N, N’-diacetate)-type ligands and their corresponding metal complexes has been successfully synthesized. This article summarizes recent progress in research on edda-type-platinum complexes. Some of these agents achieves better effect compared to the gold standard (cisplatin). It has been shown that there is a possible relationship between the length of the ligand ester group carbon chain and its cytotoxic effect. In most cases the longer the ester chain is the greater is the antitumor activity. Of particular interest are the noticeable effects of some new platinum compound with edda-type ligand on cell lines that are known to have a high level of cisplatin-resistance. Exanimate complexes appear to have a different mode of mechanism of action compared with cisplatin which includes apoptotic and necrotic cell death. There are indications that further investigations of these compounds may be very useful in overcoming the problems associated global cancer statistic.

Open access

Marija Stojanović, Ljiljana Šćepanović, Olivera Bosnić, Dušan Mitrović, Olga Jozanov-Stankov, Vuk Šćepanović, Radomir Šćepanović, Teja Stojanović, Slobodan Ilić and Dragan Djurić

Abstract

Oxidative stress appears to play a role in the pathogenesis of several inflammatory gastrointestinal diseases. Increased homocysteine levels may play a role in the pathogenesis of Chron’s disease and ulcerative colitis. The aim of this study was to examine the influence of homocysteine on the antioxidant status of rat intestine and liver. The levels of thiobarbituric acid reactive substances (TBARS), activity of catalase (CAT) and total antioxidant status (TAS) were investigated in the isolated gut and liver of young male rats in the control group (8 rats) and after 3-hоur incubation in high doses of D, L-homocysteine thionolactone (Hcy) (10 μmol/L) (8 rats). Samples of duodenum, ileum, colon and liver were homogenized in sodium phosphate buffer (1:10). Homogenates were centrifuged at 10000 for 10 min at 4° C and the supernatant was taken for biochemical assays. Our results showed that high D, L-homocysteine thionolactone concentration reduced enzymatic catalase activity in homogenates of the isolated segments of duodenum (27.04%) p<0.01; ileum (37.27%), colon (34.17%) and liver (67.46%) p<0.001. Exposition to high D,L-homocysteine thiolactone concentration significantly increased TBARS levels in the duodenum (106.05%), ileum (47.24%), colon (112.75%) and liver (32.07%) (p<0.01). Homocysteine also modifi ed the total antioxidant status of homogenates from the duodenum, ileum, colon and liver, increasing by 20.68% (duodenum), 24.74% (ileum), 14.88% (colon) and 19.35% (liver) (p<0.001). Homocysteine induced a consistent oxidative stress in rat’s intestine and liver (reduced activity of catalase and increased level of TBARS), but the elevated activity of TAS in our experiments could be explained as an adaptive response to the generated free radicals which indicates the failure of the total antioxidant defense mechanism to protect the tissues from damage caused by homocysteine.

Open access

Jelica Bjekić-Macut, Vojislav Radosavljević, Zoran Andrić, Dušan Ilić, Olivera Stanojlović, Danijela Vojnović Milutinović, Ivana Božić Antić, Marija Zdravković, Saša Hinić, Djuro Macut and Miloš Žarković

Summary

Background: Systemic inflammatory response syndrome (SIRS) changes cortisol dynamics and indicates dissociation between the adrenal cortex and the hypothalamo-pituitary unit. The aim of this study was to assess the cortisol response after stimulation with ACTH1-24 in patients with SIRS at admission to the Respiratory Intensive Care Unit (RICU) and seven days later.

Methods: Fifty-four subjects were included in the study, and SIRS was defined according to the Consensus Conference criteria from 1992. Severity of the disease was determined using the APACHE II score, and organ dysfunction using the SOFA score. Low-dose (1 μg) ACTH test (LDT) was performed in all patients, and cortisol was determined along with basal ACTH. Data were analyzed using parametric and nonparametric tests and regression analysis. The results are presented as mean ± standard deviation, and P<0.05 was considered statistically significant.

Results: There were no differences in cortisol values between the two LDTs. Cortisol increment lower than 250 nmol/L during the LDT was found in 14/54 (25.9%) subjects at the onset of SIRS. Five out of 54 (9.6%) patients died within 7 days from the onset of SIRS. Female sex and maximal cortisol response (Δ max) on LDT predicted the duration of hospitalization in RICU, while APACHE II and SOFA scores best predicted the duration of hospitalization, mortality outcome as well as overall survival outcome.

Conclusions: A difference was found in Δ max at the diagnosis of SIRS and seven days later. Δ max, and primarily the clinical scores APACHE II and SOFA predicted the outcomes of hospitalization and overall survival.

Open access

Nataša Z. Bubić Pajić, Marija N. Todosijević, Gordana M. Vuleta, Nebojša D. Cekić, Vladimir D. Dobričić, Sonja R. Vučen, Bojan R. Čalija, Milica Ž. Lukić, Tanja M. Ilić and Snežana D. Savić

Abstract

Two types of biocompatible surfactants were evaluated for their capability to formulate skin-friendly/non-irritant microemulsions as vehicles for two poorly water-soluble model drugs differing in properties and concentrations: alkyl polyglucosides (decyl glucoside and caprylyl/capryl glucoside) and ethoxylated surfactants (glycereth-7-caprylate/ caprate and polysorbate 80). Phase behavior, structural inversion and microemulsion solubilization potential for sertaconazole nitrate and adapalene were found to be highly dependent on the surfactants structure and HLB value. Performed characterization (polarized light microscopy, pH, electrical conductivity, rheological, FTIR and DSC measurements) indicated a formulation containing glycereth- 7-caprylate/caprate as suitable for incorporation of both drugs, whereas alkyl polyglucoside-based systems did not exhibit satisfying solubilization capacity for sertaconazole nitrate. Further, monitored parameters were strongly affected by sertaconazole nitrate incorporation, while they remained almost unchanged in adapalene-loaded vehicles. In addition, results of the in vivo skin performance study supported acceptable tolerability for all investigated formulations, suggesting selected microemulsions as promising carriers worth exploring further for effective skin delivery of model drugs.