Search Results

You are looking at 1 - 4 of 4 items for

  • Author: Maria Michalczyk x
Clear All Modify Search
Open access

Rajmund Sokół and Maria Michalczyk

Abstract

The aim of this study was to identify which Nosema species infect those Apis mellifera worker bees performing different functions in the colony. Samples were taken from different places inside and outside the hive, in the honey flow season. In February 2010, winter hive debris from 30 colonies was analyzed, and based on the microsporidian species identified by multiplex PCR. The following bee colonies (none of which displayed clinical symptoms of the disease) were selected for further analyses to determine the occurrence of microsporidian parasites: 1) colony A/C infected with Nosema apis and N. ceranae (mixed infection), 2) colony A infected with N. apis, 3) colony C - infected with N. ceranae, and 4) colony K - the control, which was free of infection. Between April and August, 20 nurse bees from frames of open brood, and 20 forager bees returning to the hive from pollen-collecting trips were randomly selected from each colony at 30-day intervals. The results of the study indicate that the microsporidian species is determined not only by the type of worker bee (sampling site), but also by the period (month) of the sample collection. Our findings also suggest that regardless of the type of initial infection, bees infected by different microsporidian species and bees free from infection can coexist in colonies.

Open access

Maria Michalczyk, Rajmund Sokół and Sylwia Koziatek

Abstract

Recent years have witnessed an increase in the mortality of honey bees in many regions of the world. The observed decrease in the bee population results from a combination of factors, and microsporidian parasites Nosema apis and N. ceranae are among the main contributors. Those parasites cause a microsporidian infection that shortens the lifespan of bees and reduces the productivity of bee colonies. The aim of this study was to evaluate the effectiveness of Nozevit, Api Herb and ApiX (acetylsalicylic acid + Artemisia absinthium L. extract) in the control of infections caused by Nosema spp. in a field experiment. Two groups of worker bees were evaluated - hive bees and forager bees returning to the hive. The effect of the analyzed therapies on the number of spores and the microsporidia species were analyzed by the hemocytometric method and duplex PCR. A statistical analysis revealed that the applied treatments had reduced the number of spores by 31.15% on average. In hive bees, Nosema spp. infection was most effectively reduced by Nozevit (67.85%) and ApiX (63.36%). Coinfections (N. ceranae and N. apis) were affirmed in all bee samples before treatments. However, after the treatments, single infection of N. apis and N. ceranae were detected. The tested treatments were more effective in the control of N. apis than N. ceranae.

Open access

M. Michalczyk, R. Sokół and P. Socha

Abstract

Trichomonadidae family is a protozoan occurring in different animal species. It inhabits the gastrointestinal and urinary tracts. P. hominis is rarely found in faecal samples of dogs, and its identification and differentiation from other trichomonads by light microscopy are difficult. Methods of molecular biology are the most effective in this case, because they confirm the presence of the specific species in animal organisms, irrespective of the protozoan form. The aim of this study was to find P. hominis in selected dog kennels in North-Eastern Poland. Forty-one faecal samples of dogs from 7 dog kennels were examined. The occurrence of P. hominis in 5 faecal samples of dogs with no symptoms of diarrhoea was the first one to be confirmed in Poland.

Open access

Maria Jastrzębska, Mariusz Kaczmarczyk, Małgorzata Michalczyk, Łukasz Radzimiński, Piotr Stępień, Joanna Jastrzębska, Dorota Wakuluk, Arturo Díaz Suárez, Guillermo Felipe López Sánchez, Paweł Cięszczyk, Piotr Godlewski, Paweł Król and Zbigniew Jastrzębski

Abstract

There is no clear evidence that vitamin D effectively improves physical capacity in high-level athletes. The aim of this study was to confirm that vitamin D supplementation of soccer players during eight-week high-intensity training would have a significant effect on their aerobic capacity. The subjects were divided into two groups: the experimental one that was supplemented with vitamin D (SG, n = 20), and the placebo group (PG, n = 16), not supplemented with vitamin D. All the players were subjected to the same soccer training described as High-Intensity Interval Training (HIIT). The data of the vitamin D level, PWC170, lactate threshold (LT) were collected just before and after the intervention. A significant increase in vitamin D concentration (119%) was observed in the supplemented group, while the non-supplemented group showed a decrease of 8.4%. The studied subjects improved VO2max results by 20% in the SG, and by 13% in the PG. The improvement in velocity at the LT was similar in both groups. Results of this study show that vitamin D can have a positive, though moderate, effect on aerobic performance in players subjected to high-intensity training in the form of small-sided games for 8 weeks.