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  • Author: Maria Ilie x
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Introduction: Intestinal parasitism represents an important problem of children health. Infestations with protozoa and helmints even have an increased incidence in children they weren’t observed because they were asymptomatic and atypical but with negative influence on their health regardless their immune status.

Objective: To evaluate parasitic infections in a collectivity of 38 children HIV+ from Cernavoda city (Hospis “St. Laurence”) over a period of 10 years (2002 - 2012).

Material and method: We performed two coproparasitologic examinations (in year 2002 - before antiretroviral treatment and in year 2012 - after 10 years of antiretroviral treatment) by concentration and staining methods and ELISA for Cryptosporidium parvum antigen.

Results: In our group of study the median age was 11.65 yrs. in 2002 and 20.6 yrs. in 2012. Sex ratio was M: F = 1:1.2. In year 2002 we found an incidence of parasitic disease of 94.9%, and in year 2012 we found an incidence of 26.31%. In year 2002 we found 6 cases of Giardia intestinalis, 5 cases of Blastocystis hominis, 15 cases of Chilomastix mesnili, 2 cases of Entamoeba coli, 1 case of Ascaris lumbricoides, 2 cases of Hymenolepis nana and 5 cases of poliparasitic disease. In year 2012 we found 4 cases of Giardia intestinalis, 2 cases of Entamoeba coli, 2 cases of Blastocystis hominis and one case of Isospora belli and another one case of Ascaris lumbricoides.

Conclusions: After ten years of HAART the incidence of parasitic diseases registered an important decrease from 94.9% to 26.31%.


Background and Aims Diabetes mellitus (DM) represents a worldwide health problem and a major epidemic during the past decades. The exact number of people with diabetes in Romania is unknown. The aim of this paper was to determine the number of subjects registered with diabetes in Romania. Material and Method: The Romanian Society of Diabetes, Nutrition and Metabolic Diseases (RSDNMD) asked the representatives in charge with DM in each county about the total number of subjects registered with DM, the number of subjects with newly diagnosed DM in 2011 and their treatment (insulin or oral drugs). Results: There are 803,489 persons registered with DM, with a national prevalence of 4.21%. The lowest prevalence was observed in Ilfov (0.9%) and the highest prevalence (7.83%) in Bucharest. A total of 125,574 diabetics (15.62%) are treated only with insulin while 81,910 (10.19%) with combined insulin and oral medication. In 2011 have been registered 68.294 new cases of diabetes, with an incidence of 358.63/100,000 persons. The lowest incidence was 91.15 in Giurgiu county and the highest incidence was 718.94 in Arad county. Conclusions: There are large and unexplained differences between counties for both diabetes prevalence and incidence. Consequently, RSDNMD decided to start a national epidemiological study (PREDATORR) that will be carried out in 2013.


In order to design and implement ballistic protective equipment, several common stages in developing any product must be taken (technical studies, technological demonstrators, prototypes, etc.). The final stage should be the testing-assessing of development phase, followed by the homologation of the product obtained, which is a compulsory stage. In order to characterize the properties of shock waves passing through various materials and media (air, water, materials for ballistic protection), certain techniques and working procedures were established. The most common method is testing in the shooting range where the real conditions of a detonation can be faithfully reproduced. Such tests, however, despite being the most accurate and reliable way to check the shock waves mitigation properties of materials, in addition to being extremely dangerous activities, most often require expensive materials and full-scale structures. In the first stage of development, the new materials have to be selected through the small scale tests performed in laboratory. This paper presents one test procedure that could be used to determine the capacity of the ballistic protection materials to mitigate the effects of the shock wave in laboratory conditions and at low cost.


Necrotizing fasciitis is a life threatening condition that can be quickly spread through the flesh surrounding the muscle. The disease can be polymicrobial, or caused by group A beta hemolytic Streptococci, or by Clostridium spp. We present a case of a 7 years old girl, which was hospitalized in Children Infectious Diseases Department in a 7th day of chickenpox (hematic crusts all over the body), high fever, asthenia, vomiting, oligoanuria, and tumefaction, pain and functio lessa in the right thigh. In a very short time in the right thigh swelling, edema and congestion have increased gradually, and in the third highest middle thigh the ecchymotic areas appeared evolving towards bubbles and blisters which included the right thigh and calf. After excluding the diagnosis of thrombophlebitis was raised suspicion of necrotizing fasciitis. CT pelvic scan evidenced pelvic asymmetry by maximus and medium right gluteal muscles swelling with important inflammatory infiltrate extended laterally in the subcutaneous adipose tissue. In blood culture was isolated Eggerthella lenta, and from throat swab was isolated group A Streptococci. Treatment consists of a combination of antibiotics associated with intravenous immunoglobulin administration. Despite medical treatment evolution worsened and required transfer in a pediatric surgery department where emergent surgical debridement associated with intensive antibiotic therapy was done. After this intervention evolution was slowly favorable without major limb dysfunction. Polymicrobial necrotizing fasciitis is a severe disease, which if recognized early can have a

favorable outcome.


Background: Many studies have been performed in the past few years, to determine arsenic speciation in drinking water, food chain and environment, arsenic being a well-recognized carcinogenic and toxic agent mainly in its inorganic species. The instrumental techniques used for arsenic determination, such as hydride generation atomic absorption spectrometry (HGAAS), graphite furnace atomic absorption spectrometry (GFAAS) and inductively coupled plasma mass spectrometry (ICP-MS), can provide a great sensitivity only on the total amount. Objective: The aim of this study was to develop a simple and rapid method and to analyze the concentration of total inorganic arsenic in bottled drinking water. Methods: Total arsenic was determined in samples from six different types of commercially available bottled drinking water using atomic absorption spectrometry with electrothermal or hydride generation vaporisation. All drinking water samples were acidified with 0.1M nitric acid to match the acidity of the standards. Results: The method was linear within the studied range (1-5 μg/L, R = 0.9943). The quantification limits for arsenic determination were 0.48 μg/L (HGAAS) and 0.03 μg/L (GFAAS). The evaluated arsenic content in drinking water was within the accepted limits provided by law. Conclusions: A simple and sensitive method for the quantification of arsenic in drinking water using atomic absorbtion spectroscopy was described, which can be further used in toxicological studies. As an additional advantage, the system is very fast, efficient and environmental friendly


The preventive archaeological researches of 2011 led to sensational discoveries. These include evidence for the extraction of radiocarbon data. We analyze new evidence from the periods: neolithic and eneolithic (Turdaş culture), eneolithic (Petreşti culture) and classical dacian period (1st century AD).


Non-alcoholic fatty liver disease (NAFLD) is closely associated with all features of the metabolic syndrome (MS). This strongly supports the notion that NAFLD may be the hepatic manifestation of the MS. NAFLD is currently the most common cause of abnormal liver function tests and affects approximatively 15-25% of the general population. NAFLD covers a spectrum of liver disease, from steatosis to nonalcoholic steatohepatitis (NASH) and cirrhosis. Insulin resistance (IR) has central etiologic roles in the development of MS and NAFLD, usually related to obesity. MS is frequently associated with chronic inflammation, having as principal mediators the adipocytokines and free fatty acids (FFA), but also CRP, TNF-a and IL-6. Chronic inflammation results in more IR and lipolysis of adipose tissue triglyceride stores, in enhanced hepatic glucose and VLDL production. The steatotic liver is thought to be vulnerable to secondary injuries including adipocytokines, mitochondrial dysfunctions, oxidative stress which lead to hepatocellular inflammation and fibrosis.


Doxorubicin is a widely used chemotherapeutic drug, effective on patients with acute lymphoblastic leukemia but associated with significant long term cardio-toxicity. Menadione (vitamine K3) and the flavonoid quercetin are known as strong apoptogens in human leukemia Jurkat T cells.

We explored the potential synergic cytotoxic effects of doxorubicin in association with quercetin and Menadione in this cellular model for acute lymphoblastic leukemia.

Cellular viability, apoptosis, necrosis oxidative stress and cellular cycle were determined by flow cytometry utilizing Jurkat lymphoblasts labeled with Annexin V-FITC/7-AAD, CM-H2DCFDA/7-AAD and propidium iodide respectively.

Results indicate a dose-dependent oxidative-stress generation, cell cycle arrest and apoptosis induction by doxorubicin alone, correlated with a decrease of the required doses when the anticancer drug was associated with quercetin and menadione, hence supporting the theory of an additive cytotoxic effect on leukemia cells.

Introducing QC-MD combinations in leukemia doxorubicin-based treatment could significantly increase the treatment’s efficacy. The main mechanism responsible for this effect appears to be the increase in DOX affinity for DNA, which enables lowering of the therapeutic dose.