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  • Author: Magdalena Moczała x
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Open access

Magdalena Moczała, Miriam Karpińska, Monika Poznar, Piotr Dobryszycki and Andrzej Sikora

Abstract

This paper presents utilization of argon plasma for gradual etching of calcium carbonate crystals. The plasma treatment has been chosen as it appears to be the technique that enables removal of following material layers, thus, the access to the inside of crystals is possible. Examples of investigations of the morphology and mechanical properties of surfaces of calcium carbonate crystals are presented. The impact of plasma treatment has been verified in terms of roughness and volume changes investigated using atomic force microscopy technique in a multi-step experiment. Therefore, we were able to observe the crystal degradation process, revealing the spatial inhomogeneity of the calcium carbonate crystals resulting from their core-shell structure.

Open access

Konrad Chabowski, Adam F. Junka, Tomasz Piasecki, Damian Nowak, Karol Nitsch, Danuta Smutnicka, Marzenna Bartoszewicz, Magdalena Moczała and Patrycja Szymczyk

Abstract

The suitability of low-cost impedance sensors for microbiological purposes and biofilm growth monitoring was evaluated. The sensors with interdigitated electrodes were fabricated in PCB and LTCC technologies. The electrodes were golden (LTCC) or gold-plated (PCB) to provide surface stability. The sensors were used for monitoring growth and degradation of the reference ATCC 15442 Pseudomonas aeruginosa strain biofilm in invitro setting. During the experiment, the impedance spectra of the sensors were measured and analysed using electrical equivalent circuit (EEC) modelling. Additionally, the process of adhesion and growth of bacteria on a sensor’s surface was assessed by means of the optical and SEM microscopy. EEC and SEM microscopic analysis revealed that the gold layer on copper electrodes was not tight, making the PCB sensors susceptible to corrosion while the LTCC sensors had good surface stability. It turned out that the LTCC sensors are suitable for monitoring pseudomonal biofilm and the PCB sensors are good detectors of ongoing stages of biofilm formation.