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  • Author: Magdalena Gryzińska x
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Jakubczak Andrzej, Gryzinska Magdalena, Horecka Beata, Dziadosz Katarzyna and Jezewska-Witkowska Grazyna

Abstract

The gene MYO15A is involved in the production of a protein included in the group of motor proteins known as myosins. Myosin XVA is located in the inner ear, the pituitary gland and other tissues, and has a substantial influence on the hearing process. Mutations in this gene cause amino acid substitutions in the conserved motor domain of the myosin chain, leading to shortening of the stereocilia in the hair cells, so that the function of myosin XVA is impaired. A research hypothesis was put forth that mutations in the gene responsible for the hearing process in animals of the Canidae family can cause hypoacusis, as well as substantial behavioural changes in dogs (ranging from timidity to aggressive behaviour). The study determined SNP polymorphism in a fragment of the gene MYO15A, which can cause hearing disorders or hypoacusis, in wild and farmed individuals of the Canidae family.

Open access

Andrzej Jakubczak, Magdalena Gryzinska, Beata Horecka and Grazyna Jezewska-Witkowska

Abstract

The gene IGF1 has been shown to have a significant influence on the size of individuals, including animals of the Canidae family. In this study we determined SNP mutations of the IGF1 gene in dogs, raccoon dogs and farmed and free-living red foxes from Poland and Canada. No SNP mutations were noted in dogs or raccoon dogs, but a total of 14 single nucleotide polymorphisms were identified in foxes, including 12 substitutions, as well as one new mutation missense variant (exon 6) in wild Polish foxes and one synonymous mutation variant in wild foxes from Canada. We identified specific SNP profiles characteristic only for farmed foxes and only for wild foxes, as well as specific SNP profiles or wild foxes from North America (Canada) and from Europe (Poland).

Open access

Piotr Listos, Magdalena Gryzinska, Jacek Piorkowski, Grzegorz Teresinski, Grzegorz Buszewicz, Wojciech Chagowski, Zbigniew Nozdrynplotnicki and Wojciech Lopuszynski

Abstract

The subject of the study were dogs divided into two groups according to body weight: up to 10 kg and from 10 kg to 30 kg. The aim of the study was to determine the dynamics of the post-mortem decrease in rectal and kidney temperature. The temperature was measured on both sites at the same time using a thermometer connected to a computer, under constant environmental conditions of the necropsy room. In these animals, a higher temperature in the kidneys persisted for the duration of the study. Comparative analysis between mean differences in kidney and rectal temperature in small and large dogs showed the greatest temperature amplitude in the group of small dogs, both for the kidney and the rectum. The greatest decrease in temperature, 1.2°C, was noted for the kidney in small dogs between 4 and 6 hours after death. Analysis of the dynamics of the decrease in kidney and rectal temperature for both weight groups combined, and the difference in temperature between the kidney and the rectum in the time intervals analysed showed that in the first two hours the difference between kidney and rectal temperature did not exceed 0.5°C. Two hours after death the difference in temperature between the two measurement sites was about 0.5°C after which time dropped below 0.5ºC.

Open access

Anatoliy Mazurkevych, Mykola Malyuk, Natalia Bezdieniezhnykh, Lyubov Starodub, Yuriy Kharkevych, Evgen Brusko, Magdalena Gryzińska and Andrzej Jakubczak

Abstract

Introduction: The objective of the study was immunophenotypic and cytogenetic analysis of mesenchymal stem cells from equine bone marrow and foal umbilical cords during in vitro culture.

Material and Methods: The mesenchymal stem cells were obtained from equine bone marrow of three horses and from foal umbilical cords of six foals. The cells were cultured in CO2 incubators by standard procedure. Quantitative abnormalities of chromosomes, i.e. aneuploidy and polyploidy, and structural aberrations, i.e. breaks in chromosomes and chromatid, were taken into account during the study.

Results: The results of cytogenetic analysis of equine bone marrow mesenchymal stem cells at the third and fourth passages indicated that the level of karyotype variability of these cells corresponded to the spontaneous level of karyotype variability typical of the peripheral blood lymphocytes of this species. Equine bone marrow contained several clones of stem cells that differed in the expression of specific nuclear markers characteristic of proliferating cells.

Conclusion: Mesenchymal stem cells from foal umbilical cords during in vitro cultivation are characterised by quantitative abnormalities of the chromosomal apparatus.

Open access

Andrzej Jakubczak, Magdalena Gryzińska, Beata Horecka, Kornel Kasperek, Katarzyna Dziadosz and Grażyna Jeżewska-Witkowska

Abstract

Single-nucleotide polymorphism (SNP) was analysed for selected fragments of three genes - insulin-like growth factor 1 (IGF1), myosin-XV (MYO15A) and paired box homeotic gene 3 (PAX3) - in farm and wild red foxes from two continents. The study was undertaken in order to verify whether the SNP characteristics of these genes enable farm-bred foxes to be distinguished from free-living foxes. The greatest number of changes were detected in the IGF1 gene. For each of the genes investigated specific SNP profiles characteristic only for farm foxes and only for wild foxes were noted. At the same time, specific SNP profiles were noted for wild foxes from North America and from Europe. The frequency of SNP (bases per SNP) in the gene fragments examined was 22 bp for IGF1, 34 bp for PAX3 and 56 bp for MYO15A. Single-nucleotide polymorphism is a very good molecular marker enabling characterization of nucleotide variation in the genes investigated between wild and farm individuals

Open access

Piotr Listos, Magdalena Gryzinska, Marcin Martychiewicz, Stephen Pointing, Albrecht Barton and Malgorzata Dylewska

Abstract

Caseous lymphadenitis (CLA), also called pseudotuberculosis, is a chronic and highly infectious disease of sheep and goats, subject to obligatory registration in the European Union. The EU law also applies to special territories, which include the Falkland Islands. On the Falkland Islands 99% of sheep and goats are slaughtered in one slaughterhouse, overseen by veterinary authorities, and approved for export to EU countries (mainly the UK). The export season begins about the middle of January and usually lasts 3-4 months. In the years 2013-2015 were examined 162 317 sheep, including 39 971 lambs, 35 357 yearlings and 86 989 mature rams. During the study lung tissue clippings were collected. During the 2013 season lesions were observed in 8 025 of animals (13.9% CLA). In 2014 lesions were noted in 7716 with CLA accounting for 13.91% and in 2015 lesions were noted in 5743 with CLA accounting for 11.7%. Further analysis of cases of CLA on the Falkland Islands seems interesting, as they are British overseas territories and therefore part of the European Union.

Open access

Kornel Kasperek, Beata Horecka, Andrzej Jakubczak, Brygida Ślaska, Magdalena Gryzińska, Monika Bugno-Poniewierska, Małgorzata Piórkowska and Grażyna Jeżewska-Witkowska

Abstract

The aim of this study was to detect possible differences between farmed and wild-living raccoon dogs. Analysis of polymorphism in 15 microsatellite sequences led to the conclusion that raccoon dogs raised on Polish farms and wild raccoon dogs living in Poland are two genetically distinct groups of animals. Wild Polish raccoon dogs are genetically more similar to the population of wild animals from the Kaliningrad Region than to farmed animals. The analysis of microsatellite loci showed clear genetic differences between farmed and wild-living populations of raccoon dog, despite only 50 years of isolation of the two groups of animals. The farmed population was characterized by higher genetic variation than the wild-living population. On the basis of the analyses three microsatellite loci (INU014, Ren13J22 and Ren41D20) were proposed for determination of the origin of animals that have escaped from farms.

Open access

Aneta Strachecka, Grzegorz Borsuk, Krzysztof Olszewski, Jerzy Paleolog, Mariusz Gagoś, Jacek Chobotow, Agnieszka Nawrocka, Magdalena Gryzińska and Milena Bajda

Abstract

Three groups of caged bees were fed with sugar syrup (the control), sugar syrup supplemented with amphotericin B (AmB) in a dose of 0.5 mg/ml, and sugar syrup with AmB in a dose of 0.25 mg/ ml. Amphotericin B shortened the life span of the bees and reduced the level of global DNA methylation compared to the control, however, it increased the body-surface protein concentrations. In the hindguts of the bees, there were found AmB deposits. Honeybees appear to be a useful model for studying the side effects of anti-fungal AmB therapy. Among other things, epigenetic changes and senescence acceleration are considered to be the side effects of the therapy.