An effective way to reduce nitric oxide emissions may be to reduce the level of nitrogen fertilization. Specialized formulations such as biostimulators are a good alternative to conventional mineral fertilization. The aim of the study was to determine the effect of the application of biostimulation agents (Asahi SL, Kelpak SL, Tytanit) applied against the limited dose of nitrogen (75 kgN·ha−1) on winter wheat ‘Muszelka’, their effect was measured on the parameters: grain yield, protein content, amount of chlorophyll and root mass. No statistically significant changes in yields were observed, but after application of the Asahi SL and Kelpak SL, considerably higher values were found as compared to the controls. It should be emphasised that the applied formulations significantly affected the root mass and chlorophyll content of the leaves. Especially in the case of roots of the plants where the biostimulator Asahi was used in the phase BBCH 37, Kelpak in BBCH 23 and fertilizer Tytanit in BBCH 37. And in the case of chlorophyll objects, where Tytanit was applied in BBCH 37 and 69. In the study, significant correlation was found only between the chlorophyll content and the root mass (r = 0.54).