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  • Author: Małgorzata Korbin x
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Małgorzata Korbin

Molecular Approaches to Disease Resistance in Fragaria SPP.

Resistance to economically important diseases is one of the most desired traits to have in plant crops. The Fragaria genus including 21 wild and cultivated species (Fragaria x ananassa), contains genetic sources of diseases resistance that are quite rich but not fully exploited in breeding for resistance. Usefulness of different molecular techniques and high throughput technologies for the dissection of genetic resistance mechanisms and the explanation of plant diversity in relation to pathogens at the DNA level are described in this paper. The descriptions are based on the results of different studies on genome of Fragaria that were carried out in many research institutions in the world. The proposed model of comprehensive exploration of the strawberry genome, summarized with generating resistance markers and identification of genes involved with induction or regulation of plant response to pathogen attack, appear to be very useful in breeding strawberry for resistance.

Open access

Sylwia Keller-Przybyłkowicz and Małgorzata U. Korbin

ABSTRACT

This review summarises the research projects that have been conducted with the goal of mapping the apple (Malus × domestica) genome. The generation of apple linkage and physical maps, which was started at the end of the 20th century by many scientific teams, has resulted in rich data useful for further fundamental and applied studies. The mapping efforts mainly contained the choice of mapping populations as well as the choice of types and number of markers. Over the years, scientists have achieved major successes and faced major obstacles. Many economically important traits have been mapped in the apple genome, which has a total length of 2000 cM. The recently published integrated genetic and physical maps summarise the data and will be helpful to modern breeding programs, with a special focus on resistance to various diseases, as well as on fruit quality and other desired apple traits.

Open access

Abdel-Rahman Moustafa Abdel-Wahab Mohamed, Tomasz Jęcz and Małgorzata Korbin

Abstract

This overview summarizes the research programs devoted to mapping the genomes within Fragaria genus. A few genetic linkage maps of diploid and octoploid Fragaria species as well as impressive physical map of F. vesca were developed in the last decade and resulted in the collection of data useful for further fundamental and applied studies. The information concerning the rules for proper preparation of mapping population, the choice of markers useful for generating linkage map, the saturation of existing maps with new markers linked to economically important traits, as well as problems faced during mapping process are presented in this paper.

Open access

Sylwia Keller-Przybyłkowicz and Małgorzata Korbin

Abstract

The map of the linkage groups: LG2, LG12 and LG14, which are expected to contain QTLs related to fruit quality, was generated by analysis of 56 individuals of the cross: ‘Retina’ × ‘Topaz’. Twenty three of the 27 SSR markers covered 225 cM in ‘Retina’ and 371 cM in ‘Topaz’ genome. High level of colinearity (≈85%) was found between obtained map and the respective map regions of ‘Fiesta’, ‘Discovery’, ‘Ralls Janet’ and ‘Delicious’. Only single inversions of marker positions were noted, predominantly in ‘Topaz’. CAPS and SSCP/SNP markers in seven ESTs, chosen based on the metabolic pathways of ascorbic acids and sugar, were identified. Four of the CAPS markers, linked to the genes coding: UDP glucose: flavonoid 3-o-glucosyl transferase, ascorbate peroxidase, and two sugar transporters, were mapped on LG2 (GFglTra - both cultivars), LG12 (PGiso1B and PSTS - ‘Topaz’) and LG14 (PST - ‘Retina’). According to our knowledge, loci of these markers have never been identified on the apple genome map.

Open access

Bogumiła Badek, Bogusława Napiórkowska, Agnieszka Masny and Małgorzata Korbin

Abstract

Cold temperatures in midwinter and late-spring frosts cause severe damages to strawberry plants cultivated in temperate climate regions. Despite the seriousness of the problem, the plant mechanism of defense against cold stress has not been fully elucidated yet, especially in its molecular aspect. The presented investigations were conducted on the cold-susceptible cultivar ‘Elsanta’ and the cold-tolerant cultivar ‘Selvik’. Expression profiles of three genes (CBF4, COR47 and F3H) were determined at three time-points: 0, 6 and 12 weeks after sub-zero treatment at -12 °C. The CBF4 gene was very strongly up-regulated in ‘Selvik’ plants and the highest value of the transcript level was detected just after the treatment (time - point 0). The F3H transcript in the treated ‘Selvik’ plants reached the level 4 times higher than in control plants in the 12th week after treatment (time-point 3). In ‘Elsanta’ plants, the CBF4 and COR47 genes were slightly up-regulated (time-point 2), while the F3H gene showed stability of expression. High positive correlation between the transcript level of COR47 and transcript level of CBF4 genes was observed for both cultivars.