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  • Author: M.Y. Lü x
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B. Zheng, L. Xiao, X. Wang, D. Li, Y. Lu, Y. Zhang, Q. Yan and M. Song

Abstract

To study the function of the 49 kDa excretory-secretory (ES) protein gene (P49) of Trichinella, the genes was amplified by RT-PCR from RNA of Trichinella spiralis and Trichinella nativa and several Chinese Trichinella isolates of domestic animals, and sequenced after being cloned. The amplified products of these parasites produced bands of about 950 bp. The 97.2 % to 100 % nucleotides identity and 94.3 % to 100 % identity of deduced amino acids among P49 gene of these Trichinella strains showed the close relationship of these parasites. The P49 gene of T. nativa was cloned into the BamHI site of the prokaryotic expression vector pET-30a, and the recombinant vector was expressed. The expressed product was 40.8 kDa in size. In Western blot analysis, the expressed product was reactive to sera of mice infected with T. nativa, T. spiralis and their Chinese geographical strains.

Open access

S. Piskunov, Y. F. Zhukovskii, M. N. Sokolov and J. Kleperis

Abstract

Substitution of fossil-based chemical processes by the combination of electrochemical reactions driven by sources of renewable energy and parallel use of H2O and CO2 to produce carbon and hydrogen, respectively, can serve as direct synthesis of bulk chemicals and fuels. We plan to design and develop a prototype of electrochemical reactor combining cathodic CO2-reduction to ethylene and anodic H2O oxidation to hydrogen peroxide. We perform ab initio calculations on the atomistic 2D graphene-based models with attached Cu atoms foreseen for dissociation of CO2 and H2O containing complexes, electronic properties of which are described taking into account elemental electrocatalytical reaction steps. The applicability of the model nanostructures for computer simulation on electrical conductivity of charged Cun/graphene (0001) surface is also reported.

Open access

J. Cheng, X.P. Zou, G. Zhu, M. F. Wang, Y. Su, G.Q. Yang and X. M. Lü

In this paper, the effects of position of substrates in flames, preparation time, stability of flames and catalyst precursors on the synthesis of carbon nanofibers (CNFs) by ethanol catalytic combustion (ECC) were investigated. For investigating the effects of these influence factors on the synthesis of CNFs, several sets of controlled experiments were performed, such as preparation experiments with different position of substrates in flames, different preparation time, stable and unstable flames, and different catalyst precursors. In our experiments, the catalyst precursors were iron nitrate, cobalt nitrate, nickel nitrate, and iron chloride, cobalt chloride, nickel chloride. The as-synthesized products were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and Raman spectroscopy. Our results indicate that the optimal position of substrates in flames is more than 1cm and less than 2.5cm, the optimal preparation time is more than 5min and less than 30min for massive yield, stable flames would be tent to synthesize CNFs with mainly single-type morphology and could improve the graphitization of CNFs, and the catalyst precursors obviously have effects on the synthesis of CNFs.

Open access

H.S. Tian, B.H. Zhang, S.H. Zhang and M.Y. Lü

Abstract

The Yishu Fault Zone runs through the centre of Shandong Province (E China); it is a deep-seated large fault system that still is active. Two volcanic faulted basins (the Shanwang and Linqu Basins) in the Linqu area, west of the fault zone, are exposed to rifting, which process is accompanied by a series of tectonic and volcanic earthquakes with a magnitude of 5-8. Lacustrine sediments in the basins were affected by these earthquakes so that seismites with a variety of soft-sediment deformation structures originated. The seismites form part of the Shanwang Formation of the Linqu Group. Semi-consolidated fluvial conglomerates became deformed in a brittle way; these seismites are present at the base of the Yaoshan Formation. Intense earthquakes triggered by volcanic activity left their traces in the form of seismic volcanic rocks associated with liquefied-sand veins in the basalt/sand intercalations at the base of the Yaoshan Formation. These palaeo-earthquake records are dated around 14-10 Ma; they are responses to the intense tectonic extension and the basin rifting in this area and even the activity of the Yishu Fault Zone in the Himalayan tectonic cycle.