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  • Author: M.I. Costache x
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VEGF expression in pancreatic cancer and other malignancies: a review of the literature

Abstract

Angiogenesis is a crucial event for tumor growth and it is regulated predominantly by several different growth factors. Vascular endothelial growth factor protein family (VEGF) and its receptors are probably the most important tissue factors responsible for angioblast differentiation and tube formation. VEGF protein family currently comprises several members: VEGF (or VEGF-A), VEGF-B, VEGF-C and VEGF-D, VEGF-F, placental growth factor (PlGF), and their receptors VEGFR-1, VEGFR-2 and VEGFR-3. VEGF is a key angiogenic growth factor and its level of expression is a critical marker for detection of the angiogenic diseases. The potent role of VEGF in tumor angiogenesis has been widely described in the past decade, being expressed in most types of nondigestive and digestive cancers. VEGF family members play an important role in the development of pancreatic cancer (especially VEGF-A, VEGF-C, VEGF-D, VEGFR-1 and VEGFR-2). VEGF-A is the most specific and prominent angiogenic factor among all family members and VEGFR-2 is the most important receptor in evaluating the angiogenesis in pancreatic cancer. Thus, VEGF overexpression may be considered as a diagnostic marker and as a poor prognostic factor of the disease.

Open access
Squamous Cell Carcinoma Developed in Chronic Osteomyelitis

Abstract

Introduction. Squamous cell carcinoma is the second as frequency in malignant tumors of the skin, representing 20% of this type of cancer. Inferior lip squamous carcinoma is most frequent, of 94%, because the trauma, sunburns, smoke and radiations are more frequent. Metastasis at distance in the area with chronic osteomyelitis is a rare situation. Material and method. A 78-year-old male, treated for inferior lip carcinoma, without any oncological treatment, was admitted in our clinic for a leg tumor on the anterior part, developed over a scar after osteomyelitis treated in the adolescence. The tumor growth was quick. We decided the treatment plan from the point of view of a multidisciplinary approach, plastic surgeon-orthopedistoncologist- dermatologist. A biopsy from the extraosseous tumor was performed and after the histological confirmation, the resection of the tumor block was made. For the confirmation of the bone invasion, we had to repeat the immunohistochemistry twice because the first result was negative. Until the final decision, the patient had an external fixation on the leg. A large tissue appeared on the area of resection, the aspect being suggestive for granulation. Amputation was decided after the complementary investigations and another biopsy from the granulation tissue. Results and conclusions. Squamous cell carcinoma of the lip needs prophylactic laterocervical ganglia resection for limiting metastasis.

Open access
Choanal Atresia – A Permanent Challenge in Rhinology Pathology

Abstract

Choanal atresia is the most frequent nasal congenital malformation, which, depending on the unilateral or bilateral location, has a different symptomatology. The diagnosis is confirmed through the patient’s medical history, nasal endoscopy and imagistic examination (computed tomography). The treatment is surgical and aims the (fibrous and bone) tissue disintegration which blocks the choana, and is performed through endoscopic surgical techniques. The blocked area is maintained functional by mounting a stent at the choanal tunnel level. The authors aim to describe their own experience regarding both the diagnoses as well as the treatment of this pathology, the results being compared to those from the quoted literature. The surgical technique differs depending on the type of choanal atresia: bone or membranous. The duration to maintain a fix position of the stent and the material used for the stent represent the variables which provide the success of the endoscopic surgical intervention of the choanal atresia. Subsequently, the patient is followed up at 2, 6, 12 months in the first year and then annually until 18 years of age. Choanal atresia is solved through endoscopic surgical techniques by mounting a silicone stent which shall be maintained in position for a minimum period of 3 months. The endoscopic approach of the unilateral choanal imperforation is the method of choice with minimum complications, increased success rate and fast recovery.

Open access
Eosinophil activation markers in clonal and non-clonal eosinophilia

Abstract

Eosinophils are leukocytes with multiple functions in physiologic and pathologic circumstances. Eosinophilia is typically associated with reactive conditions (helmintic infections, allergic or drug reactions and atopic disorders) and sometimes with hematologic and non- hematologic malignancies. Evaluation of a patient with eosinophilia requires numerous imaging investigations and laboratory tests for establishing the right treatment. We measured the degree of eosinophilic activation using serologic biomarkers such as serum eosinophil cationic protein (ECP) , interleukin-5 (IL-5) and eosinophilic cationic protein/eosinophil count (ECP/Eo) ratio in order to differentiate earlier among distinct eosinophilic conditions: clonal, non-clonal with malignancy and reactive eosinophilia with inflammation. The median ECP value in eosinophilic patients was significantly higher when compared to that of the control group (19.55 vs. 4.93 ng/mL, p<0.05). Within patients with eosinophilia, the clonal eosinophilia group showed a significantly higher median ECP value compared to the median ECP values of the non-clonal eosinophilia groups - (30.15 vs. 19.5 ng/mL, p<0.05 and respectively 30.15 vs. 13.3 ng/mL, p<0.05). Also patients having non-clonal eosinophilia with malignancy had a significantly higher median ECP value compared to those of reactive eosinophilia and inflammation (19.5 vs. 13.3 ng/mL, p<0.05). While ECP serum levels seemed to be a discriminatory tool for different groups of eosinophilic patients IL-5 and ECP/Eo were less useful for this purpose. However our results must be confirmed in larger studies

Open access
Compatible Biomaterials Used In Rhinology Surgery – Our Expertise

Abstract

In the last decades, rhinology has developed in a great manner concerning new surgical techniques and extending the use of compatible biomaterials in order to achieve normal morphology and normal functional aspect after surgery (endoscopic or open approach). Since biomaterials are integrated in biological active organs and systems, they have to be easily accepted by the receiver with no toxicity, inflammation or other side effects. The authors try to review all biocompatible materials used in several rhinological pathologies in order to obtain the best results after functional or reconstructive surgery. In rhinological surgery there are usually used homograft’s but the use of biomaterials like alloplastics (silicone, high density polyethylene, polytetrafluoroethylene, etc.) has widely increased. The authors present their experience in endoscopic approach of choanal atresia using nasal stents, closure techniques for septal perforation using nasal splints, endoscopic drainage and sinus ventilation using balloon sinuplasty, lacrimal pathway obstruction using double margin stent and some materials used in cosmetic and reconstructive rhinoplasty.

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Morphological characterisation and diagnostics of Xiphinema non-americanum group species (Nematoda: Longidoridae) from Romania using mutiplex PCR

Abstract

Additional data on the occurrence and distribution of Xiphinema non-americanum group species in Romania are provided. Xiphinema diversicaudatum, X. index, X. vuittenezi and X. italiae were recovered from vineyards and cherry fruit trees; adults and juvenile stages were described and analysed and the morphology/variability discussed. Multiplex PCR diagnostic test using species-specific primers designed by Wang et al. (2003) yielded amplification products with expected lengths for all screened populations of these four species. Two ribosomal markers (D2-D3 28 LSU rDNA and ITS) were sequenced and ITS RFLP patterns were obtained from two X. vuittenezi populations, which have shown some morphological differences. Comparatively low level of interpopulation genetic dissimilarity (<1 %) was revealed for both markers (for D2D3 — 0.5 %; for ITS — 0.7 %). Both populations of X. vuittenezi studied produced identical ITS-RFLP specific pattern that clearly identify this species.

Open access