Search Results

You are looking at 1 - 2 of 2 items for

  • Author: Lucian Negura x
Clear All Modify Search
Open access

Lucian Negura and Anca Negura

Abstract

Both incidence and mortality of colorectal cancer (CRC) in Romania have shown a continuous increase during the last decades. Hereditary Non-Polyposic Colorectal Cancer (HNPCC), also known as Lynch syndrome, is mainly attributable to mismatch repair (MMR) genes MSH2, MSH6, and MLH1. Individuals carrying germ-line mutations of these genes present high lifetime risk of colorectal and other cancers, compared to non-carriers. Oncogenetics is developed worldwide nowadays, for identifying hereditary predisposition to cancer and offering appropriate clinical follow-up to patients and mutation carriers in Lynch families. Molecular oncogenetic diagnosis in Lynch syndrome is based on complete Sanger sequencing of entire MMR genes, which is time and resources consuming, therefore needing an appropriate and adapted optimization. Conventional sequencing requires a sufficient number of available samples to be processed simultaneously, which increases the waiting time for diagnostic results. Complete analysis for only one patient meets difficult technical problems due to the complex co-amplification of all gene regions of interest within the same conditions, therefore increasing the costs and reducing the cost-effectiveness of the test. Here we present an original and robust technical protocol for sequencing the entire MSH2, MSH6, and MLH1 coding sequence for one patient in a single PCR plate. Our optimized and verified system overcomes all technical problems and offers a quick, robust, and cost-effective possibility to personalize molecular oncogenetic diagnosis in Lynch syndrome.

Open access

Gabriel Crumpei, Alina Gavriluț, Maricel Agop, Irina Crumpe, Lucian Negură and Ioana Grecu

Abstract

From the theoretical discussions, transdisciplinarity starts to have practical consequences in the development of programs that include consortia of universities, bringing together a large variety of professionnals who set ambitious goals, such as the Human Genome Project in the past decade, and also the Human Brain Project for this decade. We intend to present an approach in the spirit of the new paradigms of knowledge in the Human Brain Project generous program started earlier this year in Europe. A possible transdisciplinary approach on brain functions and structure is to bring a valuable, significant and innovative, unconventional contribution to the theory of multi-scale topics of the HBP project. More precisely, we intend to develop the subproject Mathematical and Theoretical Foundations of Brain Research within the HBP project. Our approach aims at exploring brain function from the perspective of the theories which influenced the last decade researches in Physics and Mathematics, such as the fractal theory, chaos and the dynamics of the nonlinear systems, in order to formulate new theories regarding brain mechanisms. We bring together models and new theories based on a principled approach to nonlinear reality. We wish to develop a new paradigm for assessing brain function, the theory of complex systems, which requires a revolutionary attitude, by rethinking how brain works and it is biologically structured. By this approach, we intend to study not only the corpuscular, but also the wave part of the matter.