Li-jun Zhang, Fei Yu and Qing-bing Ji
Jin-qian Zhang, Li-cheng Zhang, Na Ren, Ming Zhang, Li-min Guo, Xing-wang Li and Jun Cheng
Objective Patients with H1N1 virus infection were hospitalized and quarantined, and some of them developed into acute respiratory failure, and were transfered to the medical intensive care unit of Beijing Ditan Hospital, Capital Medical University in Beijing, China.
Methods The clinical features and preliminary epidemiologic findings among 30 patients with confirmed H1N1 virus infection who developed into acute respiratory failure for ventilatory support were investigated.
Results A total of 30 patients (37.43 ± 18.80 years old) with 2009 influenza A (H1N1) related acute respiratory distress syndrome (ARDS) received treatment with mechanical ventilation, 15 cases of whom were male and 17 cases died of ARDS. Fatal cases were significantly associated with an APACHE Ⅱ score (P = 0.016), but not with PaO2/FIO2 (P = 0.912) and chest radiograph (P = 0.333). The most common complication was acute renal failure (n = 9). Five patients received extracorporeal membrane oxygenation (ECMO), 3 of whom died and the others survived. The major causes of death were multiple organ dysfunction syndrome (MODS) (39%), intractable respiratory failure (27%) and sepsis (20%).
Conclusions Most patients with respiratory failure due to influenza A (H1N1) virus infection were young, with a high mortality, particularly associated with APACHE ∥ score, secondary infection of lung or type 2 diabetes mellitus.
Wei-Wei Zhang, Qing Wang, Xiang-Jun Xie and Jun-Li Si
We report a case of a 78-year-old male was admitted for 2 years of blood CA19-9 >1000 kU/L found at physical examination. Abdominal computed tomography, barium meal and gastrointestinal endoscopy did not find any malignancy. Position emission tomography indicated interstitial pulmonary fibrosis (IPF) (severe) accompanied with reactive hyperplasia of mediastinal lymph node and bilateral pleural thickening. The patient also claimed to have rheumatoid arthritis (RA) for more than 40 years. Then final diagnosis was RA with IPF. The patient’s blood CA19-9 was improved after 5-day treatment of hormone inhalation.
Yu Li-Xia, Xi Jun-Jun, Bai Qiong, Zhang Ya-ping, Li Bin and Yan Bo
The nucleotide binding site (NBS) domain sequences were isolated from genomic DNA in black bamboo, using the degenerate primer designed according to the conserved motifs of the NBS resistance gene. The expected size of the PCR product was about 700 bp. Among 55 positive clones, the amino acid sequence alignment identified 33 black bamboo resistance gene analogs (RGAs) that contain the NBS conserved motifs. All of the 33 RGAs ORFs were constructed in an NJ (Neighbor-joining) tree, and divided into 10 groups. This analysis demonstrated the diversity of the NBS class RGA in black bamboo. The maximum likelihood estimates of various evolutionary models were analyzed; the result showed that 2 groups with a total of 10 sequences and 12 sites demonstrated statistically significant positive selection. Most of the positive selected sites were not located in the NBS conserved motifs. Two groups of gene conversion events had been discovered, which provide a material basis and research direction in isolating black bamboo R genes.
Chi Zhou, Li-hua Fang, Jun-jie Deng and Wen-tao Zhang
Jun Du, Ruo Nan Li, Xin Wu and Yan Zhang
With the rapid development of shipbuilding industry exhaust world is also very harmful one kind of environmental issues, and the ship marine diesel engine exhaust gas is mainly produced, so in recent years it has developed a diesel engine SCR system. SCR system can control emissions of nitrogen oxides in the exhaust of vessel, furthermore air pollution can be reduced. The main goal of article was using fluent software to correct SCR system selection and flue gas flow under different size best deflector arrangement is simulated. Next goal is further optimize the structure of the SCR system.
Ming Zhang, Kaicheng Li, Shunfan He and Jun Wang
This paper describes the design and test of a new high-current electronic current transformer based on a Rogowski coil. For better performances, electronic current transformers are used to replace conventional electro-magnetic inductive current transformers based on ferromagnetic cores and windings to measure high-current on the high voltage distribution grids. The design of a new high-current electronic current transformer is described in this paper. The principal schemes of the prototype and partial evaluation results are presented. Through relative tests it is known that the prototype has a wide dynamic range and frequency band, and it can allow high accuracy measurements.
Ming Zhang, Kaicheng Li, Jun Wang and Shunfan He
Electronic voltage transformers (EVT) and electronic current transformers (ECT) are important instruments in a digital substation. For simple, rapid and convenient development, the paper proposed an on-site calibration system for electronic instrument transformers based on LabVIEW. In the system, analog signal sampling precision and dynamic range are guaranteed by the Agilent 3458A digital multimeter, and data synchronization is also achieved based on a self-developed PCI synchronization card. To improve the measurement accuracy, an error correction algorithm based on the Hanning window interpolation FFT has good suppression of frequency fluctuation and inter-harmonics interference. The human-computer interface and analysis algorithm are designed based on LabVIEW, and the adaptive communication technology is designed based on IEC61850 9-1/2. The calibration system can take into account pairs of digital output and analog output of the electronic voltage/current transformer calibration. The results of system tests show that the calibration system can meet the requirements of 0.2 class calibration accuracy, and the actual type test and on-site calibration also show that the system is easy to operate with convenience and satisfactory stability.
Bin Zhang, Wen-hui Lun, Xing-wang Li, Qi Wang, Jun Cheng and Yu Mao
Objective To construct prokaryotic expression vector of CFP-10 gene, and obtain recombinant protein, and the recombinant CFP-10 protein was taken as stimulus to detect specific T cell responses, to set up a method to faciliate to detect potential TB infection in China.
Methods CFP-10 was cloned into inducible prokaryotic expression vector pET-32a (+) and transfected into E. coli BL21 (DE3). After IPTG induction, the product were verified with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot hybridization were carried out to verify the antigenicity; the recombinant CFP-10 protein was taken as stimulus to detect specific T cell responses in HIV (+) persons with or without clinical manifestation of TB diseases, and HIV (-) controls with or without TB diseases.
Results The CFP-10 recombinant protein exsited in the form of inclusion body and accounted for 94% in total bacterial protein of E. coli and the molecular weight is 31 kD; Western blot confirmed the recombinant proteins had high antigenicity; our in-house ELISpot-IFN-γ assay with recombinant antigen derived from CFP-10 proteins showed significant higher frequencies in TB patients with or without HIV infection than that in the healthy controls and only HIV (+) group.
Conclusions The recombinant CFP-10 genes can be expressed successfully in prokaryotic expression system of E. coli and recombinant proteins with high antigenicity were obtained, which will set foundation for further study on their immunogenicity and bioinformatics. Our results proved that it is indeed true that some HIV positive patient have high frequencies of TB specific T cell responses, which maybe a clue to find latent TB infection in this population.
Jun-fei Zhang, Jian Fang, Hai-yan Song, Wei-li Wu, Bo Liu and Cong-xin Chen
Background Military recruits are at a higher risk of acute respiratory disease (ARD) and the causative agents might change over time, which needs to be investigated.
Methods The nasopharyngeal swabs and blood samples were consecutively collected from conscripts for three years in a military training center. The real-time fluorescent quantitative PCR assays were conducted for 15 species of common respiratory pathogens; the serum anti-Legionella pneumophila antibodies were detected by indirect immunofluorescence (IIF) assay, and serum anti-Microplasma pneumoniae antibodies, serum anti-influenza B virus and anti-influenza A virus-IgM and IgG were detected by ELISA.
Results The prevalences of ARD were 59.3% (108/182) in 2008, 23.3% (50/215) in 2009, and 19.6% (40/204) in 2010. Among the patients with ARD from 2008 to 2010, the influenza B virus infection accounted for 45.4%, 30.0% and 55.0%, and seasonal influenza A virus infection for 8.3%, 8.0% and 5.0%, respectively; the positive rates of serum anti-Legionella pneumophila and anti-Microplasma pneumoniae antibodies in recruits was lower than 10% each year respectively in the three years without diagnostic significance.
Conclusion The early appropriate diagnosis and treatment of ARD in military personnel will ensure the power strength of armed forces.