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  • Author: Li-Xin Yan x
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Open access

Wei-Han Chang, Jing-Jing Yan, Xin Li, Hai-Yan Guo and Yu Liu

Abstract

Background: Cardiomyocyte hypertrophy is a common complication of hypertension, and is recognized as an important risk factor for cardiovascular diseases. Up to now, no study has been made on the effects of telmisartan on Ang II-induced cardiomyocyte hypertrophy.

Objective: Investigate the effects of telmisartan on angiotensin II-induced cardiomyocyte hypertrophy and the phosphorylation of extracellular signal-regulated kinase (p-ERK1/2) in rat-cultured cardiomyocytes.

Methods: Rat myocardial cells were cultured. Beating rates of the cardiomyocytes, cell volumes, total protein contents, protein synthesis rates, and ERK activity were measured. The phosphorylation of p-ERK1/2 was analyzed by Western blot.

Results: Treatment of cultured cardiomyocytes with telmisartan inhibited angiotensin II-induced increases in cell volume, beating rate, total protein content and protein synthesis rate. Telmisartan markedly inhibited p-ERK1/2 phosphorylation in a dose- and time-dependent manner.

Conclusion: Telmisartan could suppress cardiomyocyte hypertrophy induced by angiotensin II. The mechanism might be related to the inhibition of p-ERK1/2 phosphorylation.

Open access

Jun Du, Ruo Nan Li, Xin Wu and Yan Zhang

Abstract

With the rapid development of shipbuilding industry exhaust world is also very harmful one kind of environmental issues, and the ship marine diesel engine exhaust gas is mainly produced, so in recent years it has developed a diesel engine SCR system. SCR system can control emissions of nitrogen oxides in the exhaust of vessel, furthermore air pollution can be reduced. The main goal of article was using fluent software to correct SCR system selection and flue gas flow under different size best deflector arrangement is simulated. Next goal is further optimize the structure of the SCR system.

Open access

Xiao-Jing Guo, Li-Li Wei, Xin-Hui Li, Ning- Ning Yu, Shao-Bo Gao, Dong-Yan Qin, Fu-Mei Chu and Kun Li

Abstract

Objective

The aim of this study was to explore the safe and effective method of expectoration in the preoperative period of patients with lung cancer resection and to promote the rehabilitation of patients.

Methods

A total of 100 cases of lung cancer patients undergoing elective surgery were divided into the observation group and the control group, with 50 cases in each group. The control group was treated with vibration expectoration vest for expectoration during the perioperative period, and the observation group was treated with respiratory function exerciser that has expectoration function in the perioperative period, three times a day, and the effect was evaluated after 5 days.

Results

The number of patients in the observation group after the first expectoration time was significantly less than that of the control group (P<0.001). Pain score, pulmonary atelectasis, and pulmonary infection rate of the observation group were significantly lower than those of the control group; the hospitalization time was significantly shorter than that of the control group; and the difference was statistically significant (P<0.05).

Conclusions

Lobectomy for lung cancer patients with perioperative respiratory training for respiratory function exercise, compared with conventional methods, is helpful for postoperative expectoration and to reduce the incidence of adverse events.

Open access

Hongyan Kou, Yutao Miao, Xunbin Pan, Li-Xin Yan, An-Li Wang and Li Lin

Abstract

We conducted an 8-week feeding trial to determine the effects of dietary starch levels on growth performance, body composition, and digestive enzyme activities of juvenile soft-shelled turtles. Six fish meal-based diets containing 120, 180, 240, 300, 360 and 420 g kg−1 cornstarch were formulated. Body weight gain (BWG), crude protein concentration in whole body, as well as protease activity increased with increasing dietary starch levels until the highest at 240–300 g kg−1, but it was decreased at higher dietary starch content. In contrast, the feed conversion ratio, moisture content and hepatosomatic index were the lowest at 240–300 g kg−1 of cornstarch. The weight gain correlated significantly with the survival rate, the crude protein composition and the protease activity, but correlated negatively to the moisture content and the hepatosomatic index. The optimal dietary starch levels for the maximum BWG and maximum protease activity were 267.25 g kg−1 and 266.79 g kg−1, respectively. The α-amylase activity was the lowest at 120 g · kg−1 of cornstarch, and increased with increasing cornstrach content. The lipase activity and the amount of crude lipid in the turtles were not influenced by dietary cornstarch. Our results suggest that around 300 g kg−1 of cornstarch in diets is optimal for juvenile soft-shelled turtles.

Open access

Jun-fei Zhang, Jian Fang, Hai-yan Song, Wei-li Wu, Bo Liu and Cong-xin Chen

Abstract

Background Military recruits are at a higher risk of acute respiratory disease (ARD) and the causative agents might change over time, which needs to be investigated.

Methods The nasopharyngeal swabs and blood samples were consecutively collected from conscripts for three years in a military training center. The real-time fluorescent quantitative PCR assays were conducted for 15 species of common respiratory pathogens; the serum anti-Legionella pneumophila antibodies were detected by indirect immunofluorescence (IIF) assay, and serum anti-Microplasma pneumoniae antibodies, serum anti-influenza B virus and anti-influenza A virus-IgM and IgG were detected by ELISA.

Results The prevalences of ARD were 59.3% (108/182) in 2008, 23.3% (50/215) in 2009, and 19.6% (40/204) in 2010. Among the patients with ARD from 2008 to 2010, the influenza B virus infection accounted for 45.4%, 30.0% and 55.0%, and seasonal influenza A virus infection for 8.3%, 8.0% and 5.0%, respectively; the positive rates of serum anti-Legionella pneumophila and anti-Microplasma pneumoniae antibodies in recruits was lower than 10% each year respectively in the three years without diagnostic significance.

Conclusion The early appropriate diagnosis and treatment of ARD in military personnel will ensure the power strength of armed forces.

Open access

Zhen-Hui Xin, Ya-Li Meng, Yan-Hua Wu, Jian Wang, Zhi-Ke Feng and Yan-Fei Kang

Abstract

A simple and coumarin-based fluorescence probe has been designed and synthesized with silyl group as recognition group of fluoride ions (F−) in this study. The results showed that the fluorescence intensity of the probe displayed prominent enhancement with addition of F− at 445 nm with incubation of 1 min. There was an excellent linear relationship between fluorescence intensity and fluoride concentration from 0 to 30 μM (0~0.57 ppm), which offered the important condition for the quantitative analysis. In addition, the highly selective response to fluorion, the low detection limit with 28 nM (0.532 ppb), low toxicity and bioimaging afforded an advantage for practical application and detecting fluoride in biological systerms.

Open access

Jiang Xiao, Yan-mei Li, Ying-xiu Huang, Wen Zhang, Wen-jing Su, Wei Zhang, Ning Han, Di Yang, Xin Li, Gui-ju Gao and Hong-xin Zhao

Abstract

Objective The aim of the study was to evaluate the characteristics of HIV drug-genotypic resistance among patients taking first-line ARV regimens using polymerase chain reaction and sequencing, and guide to design optimal ARV regimens for these patients.

Methods HIV reverse transcriptase-encoded gene was amplified with RT-PCR and amplified PCR products were aligned and comparatively analyzed with HIV resistance database to find drug-resistance mutations.

Results Twenty-eight PCR products were amplified and sequenced successfully in 30 serum samples of recruited HIV-infected patients with virologic failure. The resistance rate was 96%, mutations in NRT region were found in 26 patients (93%), while mutations in NNRT region were found in 27 patients (96%). M184V was the most common mutation (86%), K65R was selected in 14% of recruited individuals and TAMs occurred in 50% of patients, which resulted in resistance to NRTIs. Y181C and V179D were the most common mutations in NNRTIs and prevalence was 43% (12/28) and 36% (10/28), respectively, which resulted in cross-resistance to NNRTIs due to low-genetic barrier.

Conclusions Virologic failure may occur in long-term administration of first-line ARV regimens, and drugresistance mutations can be found in these patients, which resulted in resistance to first-line ARV regimens. We emphasized that HIV viral load assay and resistance assay were important tools to guide healthcare workers to design an optimal second-line ARV regimens for HAART-experienced individuals with virologic failure.

Open access

Pei-yan Zhang, Si-min Yao, Fan Zhang, Ming-xia Zhang, Wei Li, Jing Yuan, Xin-chun Chen and Bo-ping Zhou

Abstract

Objective Various immune cells in patients with CHB have been demonstrated to play critical roles in HBV infection. The goal of this study is to observe changes in Th17, Treg, Th1 and B lymphocytes from peripheral blood and to evaluate immune status of CHB patients undergoing antiviral treatment.

Methods Total of 49 CHB patients, 19 asymptomatic carriers and 29 healthy donors were included in our present study. The frequencies of peripheral Th17 cells (CD3+CD4+IL-17+Tcells), Treg cells (CD3+CD4+CD25+CD127- T cells), Th1 cells (CD3+CD4+IFN-γ T cells) and B lymphocytes in chronic hepatitis B (CHB) were analyzed by flow cytometry.

Results The frequency of Th17 cells increased after treatment for 6 months, but there was no statistically significant difference of IL-17 expression between baseline and 6 months after treatment. The frequencies of Treg cells, momory B cells and total CD19+ B cells decreased after antiviral treatment. The frequencies of Th1 cells and plasma cells increased after antiviral treatment.

Conclusions This study highlights that the reestablishment of immune function during antiviral treatment in CHB patients, which caused by the antiviral drugs or the patients themselves. CHB patients may exhibit varied responses to these antiviral drugs. It is essential to supplement immune therapy during the antiviral treatment, but Th17 may play a limited role in inflammation during antiviral treatment, targeting Th17 therapy may not be useful for CHB treatment. More time and more experiments are critical to explain it.

Open access

Zai-jun Xin, Xiu-zhen Li, Søren Nielsen, Zhong-zheng Yan, Yuan-qing Zhou, Yue Jia, Ying-ying Tang, Wen-yong Guo and Yong-guang Sun

Effect of Stubble Heights and Treatment Duration Time on the Performance of Water Dropwort Floating Treatment Wetlands (FTWS)

Floating treatment wetlands (FTWs) with Water Dropwort (Oenanthe javanica) were established in winter to investigate their potential role in the purification of eutrophicated water, and to identify the effects of different stubble heights of the Water Dropwort on the performance of the FTWs. The results of the experiments demonstrated: The Water Dropwort FTWs were effective in buffering the pH of the experimental water. The Water Dropwort FTWs were efficient in purifying eutrophicated water, with removal rate for total nitrogen (TN), total phosphorus (TP), ammonium nitrogen (NH4 + - N), and nitrate nitrogen (NO3 - - N) at 91.3, 58.0, 94.6, and 95.5% in the 15-day experiment, respectively. No significant difference in the purification effect was found among different stubble heights of Water Dropwort FTWs. Significant differences between the zero control and the FTWs were found for the removal of TP in the first 11 days; and for the removal of NH4 + - N in the first 4 days. No significant difference was found between the zero control and the FTWs for NO3 - - N in the first 4 days, but significant difference was detected after day 4. The optimum treatment duration time for the FTWs with Water Dropwort will depend on the nutrients to be removed. These results will provide basis for further application of the FTWs at large scale, as well as for future studies on the mechanism of nutrient removal process.