This paper presents the results of research focused on the attitudes of schoolgirls at the 5th and 9th grade of primary schools towards physical and sport education. The aim of this project was to find out the level of the attitudes of schoolgirls at primary schools in the Bratislava Region. The research sample consisted of 247 schoolgirls attending urban and village primary schools. The basic research tool we have used was the standardized attitudinal questionnaire designed for the primary schools pupils. We have found out that in the 5th and 9th grade of primary schools the positive attitudes toward physical and sport education prevail over the indifferent attitudes. At primary schools in the Bratislava Region, the schoolgirls in the 5th grade showed more positive attitudes than schoolgirls in the 9th grade.