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  • Author: Larysa B. Bondarenko x
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Ganna M. Shayakhmetova, Larysa B. Bondarenko, Anatoliy V. Matvienko and Valentina M. Kovalenko

Abstract

Objectives: To evaluate the effect of anti-tuberculosis drugs (ATD) on indices of reproductive capability, DNA fragmentation and offspring development of male rats with testicular malfunction caused by experimental diabetes. Materials and Methods: Wistar albino male rats (body weight 160-200 g) were divided into three groups: I - control, II - streptozotocin diabetes, III - streptozotocin diabetes + ATD. The testis DNA fragmentation was determined electrophoretically; spermatogenetic indices, offspring antenatal and postnatal development indices - by standard procedures. Morphological analyses of gonadic structures were carried out by optic microscopy. Results: The study of the effects of diabetes and ATD administration on testis cells morphologic and morphometric parameters and spermatogenesis suggested the presence of specific diabetes- and anti tuberculosis drugs - mediated quantitative and qualitative changes in male rat reproductive organs, spermatogenetic epithelial cells, level and character of DNA fragmentation in comparison with normal rats. These changes were accompanied by alterations in processes of fertilisation (with intact females), embryogenesis and by lowering of offspring survival. Conclusions: Observed changes could hence affect the state and correct functioning of spermatogenetic epithelium and of other tissues of reproductive organs, as well as offspring development in diabetic rats.

Open access

Ganna M. Shayakhmetova, Larysa B. Bondarenko, Valentina M. Kovalenko and Volodymyr V. Ruschak

This study is a complex investigation of alcohol-mediated changes in CYP2E1 mRNA and protein expression in the testes, as well as spermatogenesis indices and type I collagen amino acid contents, in male rats. Wistar albino male rats were divided into two groups: I - control (intact animals), II - experimental (chronic alcoholism, exposure to a 15 % ethanol aqueous solution during 150 days). The destructive changes in the spermatogenic epithelium were accompanied by a decrease in sperm number and motility time. CYP2E1 mRNA and protein expression were elevated in the testes 3 and 1.4 times, respectively. Also, significantly lower contents of lysine, glutamic acid, serine, proline, alanine, valine, and phenylalanine residues accompanied by an increase of hydroxyproline, glycine, and threonine residue contents were detected in the skin type I collagen of the experimental group. Chronic ethanol consumption caused testicular failure along with an overexpression of CYP2E1 mRNA and protein in the testes as well as quantitative changes in type I collagen amino acid contents. The profound alcohol-mediated changes in collagen type I amino acid contents may have affected the spermatogenic epithelium state. The modulation of testicular cytochrome P450 2E1 mRNA and protein expression could change the functioning of this isozyme in target organs and take part in the mechanism of ethanol gonadotoxicity.

Open access

Ganna M. Shayakhmetova, Larysa B. Bondarenko, Anatoliy V. Matvienko and Valentina M. Kovalenko

ABSTRACT

There is good evidence for impairment of spermatogenesis and reductions in sperm counts and testosterone levels in chronic alcoholics. The mechanisms for these effects have not yet been studied in detail. The consequences of chronic alcohol consumption on the structure and/or metabolism of testis cell macromolecules require to be intensively investigated. The present work reports the effects of chronic alcoholism on contents of free amino acids, levels of cytochrome P450 3A2 (CYP3A2) mRNA expression and DNA fragmentation, as well as on contents of different cholesterol fractions and protein thiol groups in rat testes. Wistar albino male rats were divided into two groups: I - control (intact animals), II - chronic alcoholism (15% ethanol self-administration during 150 days). Following 150 days of alcohol consumption, testicular free amino acid content was found to be significantly changed as compared with control. The most profound changes were registered for contents of lysine (-53%) and methionine (+133%). The intensity of DNA fragmentation in alcohol-treated rat testes was considerably increased, on the contrary CYP3A2 mRNA expression in testis cells was inhibited, testicular contents of total and etherified cholesterol increased by 25% and 45% respectively, and protein SH-groups decreased by 13%. Multidirectional changes of the activities of testicular dehydrogenases were detected. We thus obtained complex assessment of chronic alcoholism effects in male gonads, affecting especially amino acid, protein, ATP and NADPH metabolism. Our results demonstrated profound changes in testes on the level of proteome and genome. We suggest that the revealed metabolic disorders can have negative implication on cellular regulation of spermatogenesis under long-term ethanol exposure.

Open access

Larysa B. Bondarenko, Ganna M. Shayakhmetova, Anatoliy V. Matvienko and Valentina M. Kovalenko

Abstract

Objectives: To investigate the effects of diabetes on the reproductive system and extracellular matrix proteins of diabetic rats. Materials and Methods: Wistar albino male rats, body weight (BW) 160-200 g, were divided into two groups: I - streptozoticin diabetes, II - normal non-diabetic animals. The content of amino acids in rat type I collagen was determined using an amino acid analyzer. Morphological analyses of gonadic structures were carried out by an optic microscope. Results: The study of the effects of diabetes on type I collagen amino acid content, testis cells morphologic and morphometric parameters and spermatogenesis demonstrated the presence of diabetes-mediated quantitative and qualitative changes in male rat reproductive organs, spermatogenetic epithelial cells and extracellular matrix proteins in comparison with normal. Conclusions: Observed collagen molecules changes could hence affect the properties and correct functioning of spermatogenetic epithelium and of other tissues of reproductive organs. They could be caused by diabetes via deficiency of insulin which is involved in collagen synthesis regulation at different stages of this process, cytochrome P450-2E1 induction and reactive oxygen species effects on protein biosynthesis processes.