Katarzyna Ognik, Krzysztof Patkowski and Eugeniusz R. Grela
The experiment was conducted on lambs of the Polish Lowland sheep (PLS) and a synthetic BCP line. The redox potential was assessed in blood of lambs with different genotype and sex, which were fed feed mixtures with various contents of a proteinxanthophyll (PX) concentrate from alfalfa (1.5% and 3%). The 1.5% addition of PX concentrate to feed stimulated the response of the antioxidant defence mechanisms of the lambs to a greater extent than the 3% addition. The study showed that sex of the lambs had no significant effect on changes in the redox parameters of blood. The PLS genotype of lambs determined higher values of superoxide dismutase (SOD), total antioxidant potential of plasma (FRAP), malondialdehyde, and vitamin C than the BCP genotype. Along with the growth of the lambs, a descending tendency was observed in SOD and catalase levels, and an ascending tendency in FRAP value.
Katarzyna Ognik, Krzysztof Patkowski, Tomasz Gruszecki and Krzysztof Kostro
The aim of the study was to determine the effect of the perinatal period on redox status indicators in the blood of ewes before and after lambing and during lactation. The study was performed on 12 ewes of the synthetic SCP line. Blood for testing of redox parameters was collected seven times: before pregnancy, 1.5 months and 24 h before lambing, 2 and 24 h after lambing, and in the fourth and eighth weeks of lactation. The following blood indices were determined by spectrophotometry: lipid peroxides, malondialdehyde, superoxide dismutase, catalase, plasma total antioxidant capacity, uric acid, urea, bilirubin, and creatinine. The tests showed that during the perinatal period reactions are generated which lead to oxidative stress. Oxidative stress in pregnant ewes was found to increase during the period before lambing and may persist even up to weeks 4-8 of lactation.
Urszula Kosior-Korzecka, Krzysztof Patkowski, Ryszard Bobowiec, Marta Wójcik and Elżbieta Tusińska
The aim of the study was to analyse the effects of maternal obesity and the litter size on the growth rate and plasma concentrations of GH, IGF-1, insulin, and glucose in ewe lambs during the first 6 weeks of their postnatal life. Seventy-six SCP sheep: 35 ewes and 41 female offspring were used. Before gestation, the ewes were divided into two groups: N - normally weighing and O - obese sheep. After the parturition, the lambing rate and the birth type were estimated. The born female lambs were separated into five groups: IN - singleton and IIN - twin offspring of normally weighing mothers; IO - singleton, IIO - twin and IIIO - triplet offspring of obese mothers. They were weighed at birth and weekly thereafter, until the completion of the 6th week of their postnatal life. Afterward, daily weight gains and concentrations of biochemical parameters were analysed. No significant differences in GH, IGF-1, insulin, and glucose levels between the groups of lambs born to non-obese and obese sheep, both carrying singletons and twins, were found. In contrast, significantly increased concentrations of GH (P≤0.001), insulin (P≤0.001), and glucose (P≤0.05) and markedly dropped level of IGF-1 (P≤0.001), as well as reduced daily body mass gains in triplets in comparison to other groups of lambs were observed. Maternal obesity caused significant lambing rate’s accretion with the rise in triplets’ frequency. However, in ewe lambs of this birth type, the disrupted relationships between plasma levels of GH, IGF-1 and growth rate, and between plasma levels of insulin and glucose were found.