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Open access

Zdzisław Zakęś, Maciej Rożyński, Elżbieta Ziomek and Krystyna Demska-Zakęś

Abstract

The aim of the study was to determine the impact of intramuscular tagging with passive integrated transponders (PIT tags) on the basic condition and hematological and biochemical indexes of the blood plasma of juvenile European perch (initial body weight of approximately 80 g). Throughout the observation period, i.e., for 42 days following PIT implantation, the procedure was not noted to have had a negative impact on fish growth, condition, or feed conversion ratio. The blood plasma biochemical indicators analyzed did not differ statistically significantly between the fish tagged with PIT and the untagged control group. No significant impact from this procedure was noted in the hematological indexes tested. Only with regard to the mean corpuscular hemoglobin (MCH) parameter was the value significantly lower in the fish tagged with PIT than in the control group (P ≤ 0.05). The difference determined was 1.40 pg (28.78 vs. 30.18). The MCH level in the fish tagged with PIT most probably was within the norm for this species. High tag retention (100%) and the lack of any significant impact on condition or hematological and biochemical indexes (with the exception of MCH) permits recommending this tagging method for use in juvenile perch.

Open access

Zdzisław Zakęś, Maciej Rożyński, Andrzej Kapusta, Elżbieta Ziomek and Krystyna Demska-Zakęś

Abstract

The aim of the study was to determine the impact the surgical implantation of acoustic transmitters (AT; implantation incisions were sutured with tissue adhesive) had on the condition and health of pikeperch, Sander lucioperca (L.), (initial body weight of approximately 600 g). After tagging, the fish were held for 42 days in recirculating aquaculture systems. During this period, no negative effects were observed stemming from the implantation of AT on fish growth, condition, survival, or foraging effectiveness. The procedure also did not have a negative impact on hematological indicators. Among the 13 biochemical blood plasma indicators analyzed, only glucose level in the fish tagged with AT was significantly lower than that in the control group (P≤0.05). The rate of wound healing and the condition of the implantation incisions raised no concerns. Three weeks following the AT implantation procedure the incisions were closed and no infection was noted. Short-term AT retention (42 days) was 83.3%, and the fish shed the tags in the first two weeks following implantation.

Open access

Teresa Wlasow, Krystyna Demska-Zakes, Piotr Gomulka and Sylwia Jarmolowicz

Various aspects of piscine toxicology

In opposition to toxicology of mammals piscine toxicology is closely connected with the conditions of external environment. The aquatic environment is necessary for embryonic development and after hatching during short or long-lasting larval period of most fish species. An aquatic environment is polluted by many industrial and agricultural wastes. Ammonia as a toxic and common compound in water have negative influence for aquaculture especially in intensive fish culture, recirculation system and hatchery facilities. Acute toxicity of ammonia was investigated in carp Cyprinus carpio L. and developmental stages of chub Squalius cephalus L. Changes in the peripheral blood characteristics and hemopoietic tissues of carp occurred after exposition to ammonia in acute tests and 3, 5 and 10 weeks sublethal concetration. The observed increase of the concentration of most amino acids in fish intoxicated with amonia suggests that the process reflects detoxication of ammonia which takes place both in the brain and muscles after 3 weeks of exposition. Phenol intoxication tests induced considerable unfavorable changes in the blood and dystrophic and necrobiotic lesions in tissues of fish leading to dysfunction both hemopoietic and reproductive processes.

In study on fish reproduction disruptors the influence of oxygenated polycyclic hydrocarbons (17-β-estradiol, 4,7-dihydroxyisoflavone, 1,6-dihydroxynaphthalene and 1,5-dihydroxynaphthalene) and oxygenated monocyclic hydrocarbons (phenol, 4-n-heptylphenol, 4-n-buthylphenol, 4-sec-buthylphenol; 4-tert-buthylphenol) was assessed using histopathological methods. It was established that examined oxygenated aromatic hydrocarbons both natural (17-β-estradiol and 4,7-dihydroxyisoflavone) and synthetic can disrupt the differentiation of primary and secondary sex traits in pikeperch Sander lucioperca L. The chronic activity of these "biomimetics of estrogen" can lead to the disappearance of natural fish population. In vivo and in vitro tests were used to exam dibutyl phthalate and butyl benzyl phthalate impact on the development of the reproductive system of pikeperch. Additional as multigenerational studies are needed to clarify influence long term exposure of fish to environmental concentrations of endocrine disrupting chemicals.

Hydrogen peroxide used in fish therapy is known to be toxic for sensitive species. In our work safe concentrations and exposure times was evaluated for ide Leuciscus idus L. and pike Esox lucius L. fry. The intensity of lesions in gills, skin, pseudobranch and thymus of exposed fish were connected with the time of bath.

Actually anesthetics are routinely required during stressful procedures with fish, but data regarding the safety of individual anesthetics to different fish species are still few and insufficient. The influence of clove oil, MS-222 and 2-phenoxyaethanol anesthesia on fish organism was investigated in our faculty with cooperation with Research Institute of Fish Culture and Hydrobiology, Vodnany, Czech Republic.

Open access

Zdzisław Zakęś, Krystyna Demska-Zakęś, Mirosław Szczepkowski, Maciej Rożyński and Elżbieta Ziomek

Abstract

The aim of the study was to determine the impact of diet and sex on the hematological and blood plasma biochemical profiles and the liver histology of pikeperch, Sander lucioperca (L.) reared in recirculating aquaculture systems (RAS) (initial mean body weight (BW) 1.35 kg). The proximate composition of the two commercial feeds used were (protein/lipid/nitrogen-free extracts) (P/L/NFE)) P505/L118/NFE294 g kg−1 (group I) and P471/L141/NFE290 g kg−1 (group II). Neither diet nor sex had a significant impact on final fish body weight (≈ 2.0 kg). Sex was noted to significantly impact glucose content (Glu – higher in males) and cholesterol (Chol – higher in females) in the blood plasma. Diet was confirmed to have a significant impact on levels of hematocrit (Ht), hemoglobin (Hb), and mean corpuscular hemoglobin (MCH), and the values of these indicators were higher in group I. Sex had a significant impact on Ht, Hb, MCH, and mean corpuscular hemoglobin concentration (MCHC), with higher values in male pikeperch. Diet and sex had significant impacts on the values of the pikeperch hepatosomatic index (HSI), hepatocyte size and that of their nuclei, and the values of the nucleocytoplasmic index (NCI).

Open access

Maciej Rożyński, Andrzej Kapusta, Krystyna Demska-Zakęś, Elżbieta Ziomek, Andrzej Szczerbowski, Konrad Stawecki and Zdzisław Zakęś

Abstract

The aim of this work was to determine the impact on European perch, Perca fluviatilis L. (mean body weight – 78.33 g) of the intraperitoneal implantation of telemetry transmitters using different suturing methods. In the first experiment silk sutures were used (experiment I – group ST), while in the second tissue adhesive was used (experiment II – group GT). Following the procedure, the fish were kept for 42 days in a recirculating system. Differences in growth and condition parameters were only noted in the first week of the experiment. Specimens from group GT had lower values for DGR (daily growth rate) and SGR (specific growth rate), but a higher value for FCR (feed conversion ratio) values. For the hematological parameters, lower values of MCV (mean corpuscular volume) and PLT (blood platelets) were noted in group GT, while for the biochemical parameters, lowered ALP (alkaline phosphatase) activity and Mg (magnesium) concentrations were noted in group ST. In group ST, 33.3% of the specimens loss their tags, while in group GT 77.8% did so. Differences in incision healing were only noted in the second week, when specimens in group ST were observed to have fully closed incisions, while in group GT 50% of the incisions were open. Despite the high percentage of implantation incision healing in both groups, because of the high values of tag loss rate, neither method can be recommended for perch. It might be more effective to use tag with external antennae in this species. The method use for closing implantation incisions also must be improved to eliminate tag shedding.

Open access

Maciej Rożyñski, Krystyna Demska-Zakęś and Dorota Fopp-Bayat

Abstract

The aim of the study was to determine the impact of triploidization on hematological and blood gas parameters in Siberian sturgeon, Acipenser baerii Brandt. The study material was comprised of juvenile Siberian sturgeon specimens with total body lengths of 22.3 ± 2.9 cm and mean body weights of 75.3 ± 40.7 g. Triploidization was confirmed to result in significant changes in the hematological profile of Siberian sturgeon. In comparison to the diploid group, the fish with higher levels of ploidy exhibited lower erythrocyte counts, increased hemoglobin concentration, and increased values of mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration. A significant decrease in the percentage share of lymphocytes and an increase in the percentage share of granulocytes in the peripheral blood were also observed, which indicate disturbances in the immune response (particularly in the adaptive immune system) and greater susceptibility to stress. Triploidization did not, however, cause significant differences in the blood gas profile.