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  • Author: Kristina Matković x
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Airborne Fungi in Dwellings for Dairy Cows and Laying Hens

The air of animal dwellings can contain great amounts of bioaerosol composed of dust, bacteria, fungi, and endotoxins. The composition may depend on animal species, building construction, animal accommodation, and microclimate parameters, to name just a few factors. Pathogens contained may be a serious threat to animal and human health.

The aim of our study was to analyse the fungi aerosol content in a stable housing dairy cows and in a coop for laying hens over the three autumn months of 2007. The air was sampled on Petri dishes with Sabouraud glucose agar. After laboratory treatment, we identified the most common fungi. Their count in the stable ranged from 3.98×103 CFU m-3 to 5.11×104 CFU m-3 and in the coop from 6.89 ×104 CFU m-3 to 1.13×105 CFU m-3. The difference between the two animal dwellings was statistically different at the level of p<0.05. In both dwellings, the most common were the fungi Aspergillus sp., Penicillium sp., and yeasts, followed by Cladosporium sp., Fusarium sp., Mucor sp., Scopulariopsis sp., Alternaria sp., and Rhizopus sp.

Our results are entirely in line with values reported in literature and are at the lower end of the range. They call for further investigation that would eventually lead to setting air quality standards for animal dwellings and to developing reliable monitoring systems in order to ensure safe food and safe environment.

Determination of Aspergillus Fumigatus Allergen 1 in Poultry Farms Using the Enzyme Immunoassay

Poultry farms contain high levels of allergenic fungi, and Aspergillus spp. is the most common genus of moulds. Aspergillus fumigatus antigens are responsible for the development of several respiratory diseases including asthma. The aim of this study was to measure the mass fraction of Asp f 1, a major allergen of Asperillus fumigatus in 37 indoor dust samples collected from four poultry farms in a rural area of the Zagreb County (Croatia) using the enzyme-linked immunosorbent assay. More than 62 % of dust samples had detectable Asp f 1 levels (limit of detection 3.6 ng g-1). The overall mean Asp f 1 level was 17.9 ng g-1 [range (3.8 to 72.4) ng g-1]. Satisfactory results were obtained for analytical within-run imprecision (6.7 %), between-run imprecision (10.5 %), and accuracy (91 % to 115 %). Microclimate parameters (air temperature, relative humidity, and velocity) were within the recommended ranges in all poultry farms. This study has shown that Asp f 1 settles on dust at poultry farms and that occupational exposure to this allergen deserves monitoring in livestock buildings.


Introduction. High-category hotels are considered to be one of the most important elements of every tourist destination’s supply. Recently, the lack of highly rated hotels in the Republic of Croatia has often been criticized by the public because they are believed to be a prerequisite in the creation of a competitive tourist offer.

Material and methods. The authors have performed a multi-criteria decision analysis of eight companies owning five-star hotels in Primorsko-Goranska County using the Analytic Hierarchy Process (AHP) method. Their business performance was evaluated based on both financial and market criteria. The financial criteria encompassed indicators of liquidity, indebtedness, activity and profitability. Data regarding the market indicators were collected using a questionnaire.

Results. The importance of market criteria in the tourism industry is visible in tourist demand, which is founded on the perception of potential hotel guests, their recognition of the brand and thus the market potential of the hotels themselves. Therefore, both the financial and market aspect have been included in the Analytic Hierarchy Process analysis and synthesized to estimate the overall priority based on which the hotels were ranked. According to all criteria and sub-criteria, the best ranked hotel company is Eles Turizam j.d.o.o with its five-star hotel Mozart (0.2540), followed by Milenij hotels d.o.o. with its Milenij and Milenij Sveti Jakov. The third place has been assigned to Liburnija Rivijera Hoteli d.o.o. and its Ambasador.

Conclusions. All but one of the analyzed five-star hotels are under the ownership of the biggest hotel companies in PGŽ with a long presence in the tourism industry. Therefore, it was expected that, due to their market recognizability and, consequently, their good financial performance, they would rank first. However, the research has shown that market potential does not necessarily imply best practices. Eles Turizam and its hotel Mozart were ranked the highest according to financial and overall priority scores. It may thus be concluded that even a small family hotel company with reduced marketing costs, especially one with an elite tourist offer, can compete with big hotel companies if it continuously assures the quality of its services.


Good air quality in poultry houses is crucial for animal health and productivity. In these houses, air is generally contaminated with noxious gases and microorganisms, the concentrations of which depend on numerous factors including microclimate. In this case study, the relation between microclimate and air concentrations of noxious gases and microorganisms was investigated in extensively reared turkey house. The study was carried out at a family household in Dalmatia hinterland, Croatia, with 50.3±3.1 turkeys kept in the house during the study period. Air temperature, relative humidity, airflow rate, concentrations of ammonia, carbon dioxide, bacteria and fungi in indoor air were measured three times per month from September to December, in the morning, prior to releasing turkeys out for grazing. Air temperature ranged from 9.73 to 26.98 °C, relative humidity from 63.29% to 75.08%, and airflow rate from 0.11 to 0.17 m/s. Lowest ammonia and carbon dioxide concentrations were measured in September (2.17 ppm and 550 ppm, respectively) and highest in December (4.50 ppm and 900 ppm, respectively). Bacterial and fungal counts were lowest in December (2.51×105 CFU/m3 and 3.27×103 CFU/m3 air, respectively) and highest in September (6.85×105 CFU/m3 and 1.06x105 CFU/m3 air, respectively). Air temperature and relative humidity showed negative correlation with concentrations of noxious gases and positive correlation with air microorganisms (P<0.05 all).


In the present study, postural behaviour was compared between gilts kept in service unit with different types of flooring during all seasons. The study included four 28-day production cycles and 10 gilts per cycle, equally divided into a control and an experimental group. Control gilts were housed in gestation stalls with slatted concrete floor, whereas in the experimental group the floor was covered with an adjusted rubber mat. Postural behaviour of gilts was observed 4 times per cycle for 4 hours. Study results showed that during cooler seasons, gilts in concrete stalls spent more time standing and lying sternally, whereas gilts in matted stalls were mostly lying, predominantly laterally (P<0.001 all). There were no significant between group differences according to the time the gilts spent sitting or the frequency of changing posture in any season observed. Nevertheless, experimental animals spent significantly less time changing standing to both lying positions during all seasons (P<0.01 all). In conclusion, rubber mats may improve lying comfort in gilts; however, when using rubber mats, the house thermal conditions should be taken in consideration.