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Open access

Nüket Sivri, Arzu Funda Bağcıgil, Kemal Metiner, Dursun Zafer Şeker, Selin Orak, Sevgi Güneş Durak and Vildan Zülal Sönmez

Abstract

This study is aimed to investigate culturable airborne bacteria concentrations and the composition of methicillin-resistant staphylococci in eleven different locations on the basis of specific activities conducted within different parts of the European side of Istanbul. The highest bacterial levels were observed at the Bakirkoy station (1 100 CFU/m3) while the second highest levels were found at the Bahcelievler station (1 040 CFU/m3) in October; the lowest levels (10 CFU/m3) were measured at other different stations (Atakoy, Yesilkoy). Fifteen methicillin-resistant isolates [Staphylococcus hominis (n=11), S. cohnii spp. cohnii (n=2), S. sciuri (n=1), S. capitis spp. capitis (n=1)] were identified. The disc diffusion method was used to identify the antimicrobial resistance of these isolates, it was observed that the most common resistance was to penicillin (P) (n=11), doxycycline (DO) (n=4) and tetracycline (T) (n=5). None of the isolates was resistant to imipenem, amoxicillin/clavulanic acid, vancomycin (IPM, AMC, VA). However, multiple antimicrobial resistance was found to be 26.7%. The results of this study revealed the importance of isolated methicillin-resistant staphylococci in the stations with densely active human population and traffic, for public health. As a result, the importance of resting along known shorelines, where culturable airborne bacteria concentrations are much lower, and its importance for human health have been emphasized.

Open access

Mehmet Cemal Adiguzel, Belgi Diren Sigirci, Baran Celik, Beren Basaran Kahraman, Kemal Metiner, Serkan Ikiz, A. Funda Bagcigil, Seyyal Ak and N. Yakut Ozgur

Abstract

Introduction

The study aimed to isolate thermophilic Campylobacter from chickens raised three rearing methods, determine its antimicrobial susceptibilities, and examine resistance-related genes by PCR.

Material and Methods

Cloacal swabs or intestinal contents were taken in Istanbul, Sakarya, and Izmir provinces. Chickens were from small village-based family-run businesses (n = 70), organically raised (n = 71), and conventionally raised broilers (n = 79). The samples were cultured on modified charcoal cefoperazone desoxycholate (mCCD) agar. Suspect isolates were identified with multiplex PCR (mPCR). As per EUCAST standards, MIC values were derived by broth microdilution for tetracycline, ciprofloxacin, nalidixic acid, kanamycin, gentamicin, and erythromycin in isolates of C. jejuni (n = 98) and C. coli (n = 83).

Results

In C. jejuni, 78.6% tetracycline, 87.8% ciprofloxacin, and 81.6% nalidixic acid resistance was detected, but none was to kanamycin, gentamicin, or erythromycin. In C. coli, 98.8% ciprofloxacin and 63.9% nalidixic acid resistance was detected, whereas resistance to non-quinolones was not observed. C257T (Thr-86-Ile) mutation in the gyrA gene of all phenotypically quinolone-resistant isolates was detected through a mismatch amplification mutation assay PCR (MAMA-PCR). It emerged that all isolates bore the tet (O) resistance gene.

Conclusion

Common tetracycline, nalidixic acid, and ciprofloxacin resistance exists in Campylobacter isolated from chickens raised three rearing methods.