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  • Author: Katarzyna Żarczyńska x
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Open access

Katarzyna Żarczyńska, Przemysław Sobiech, Anna Snarska, Dawid Tobolski, Chandra Shekhar Pareek and Dariusz Bednarek



In calves, hyposelenosis degenerates skeletal muscles in different parts of the body. The extent of damage to muscle cells can be diagnosed by determining the activity of creatine kinase (CK), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH). The aim of this study was to analyse variations in the serum levels of LDH isoenzymes in calves with nutritional muscular dystrophy (NMD), to determine the applicability of this parameter for diagnosing NMD, and to describe the influence of hyposelenosis on total protein (TP), triglyceride (TG), and cholesterol (CHOL) levels.

Material and Methods

Two groups of calves (n = six animals per group) were used. After birth, control group calves (SC) were intramuscularly administered 10 ml of a preparation containing selenium (Se) and vitamin E, and experimental group animals (SE) that were not injected. Blood was collected after 5, 15, and 25 days, and the concentrations of Se, vitamin E, TP, TG, and CHOL and the activity of glutathione peroxidase (GSH-Px), CK, and LDH fractions were determined.


Hypocholesterolaemia and elevated TG levels were found in SE group calves whose LDH fractions revealed a significant increase in LDH4 and LDH5 activity and a decrease in LDH1 activity when electrophoretically separated.


Nutritional muscular dystrophy is accompanied by hypocholesterolaemia and elevated TG levels caused by muscle lipolysis. LDH4 and LDH5 activity parameters assist early diagnosis of NMD in calves.

Open access

Justyna Radwińska, Anna Domosławska, Andrzej Pomianowski, Katarzyna Żarczyńska and Andrzej Jurczak


Twenty bitches with acute endometritis-pyometra complex (EPC) and 20 clinically healthy bitches were examined. The following coagulation parameters were determined in haemostatic evaluations: prothrombin time (PT), activated partial thromboplastin time (aPTT), thrombin time (TT), fibrinogen concentrations (FBG), D-dimer concentrations (D-D), antithrombin activity (AT), and blood platelet counts (PLT). Morphological and biochemical blood parameters were also analysed. Examinations of animals affected by EPC revealed blood coagulation and fibrinolytic disorders, and the noted results (PT 13.7 ±1.06 s, aPTT 23.4 ±1.04 s, TT 15.6 ±0.68 s, FBG 2.2 g/L, D-D 785.4 ±103.05 μg/L, AT 111.1 ±13.51%, PLT 169.30 ±126.31 103/μL) point to a high risk of disseminated intravascular coagulation. The findings indicate that the coagulation parameters of bitches affected by EPC should be analysed before treatment as the noted disorder can significantly complicate therapy and ovariohysterectomy, and endanger the patients' life.