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  • Author: Katarina Rajković x
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Snježana Mirković, Katarina Rajković, Sanja Jeremić, Marijana Gavrilović, Ljiljana Tomić, Valentina Arsić Arsenijević and Boro Krstić

Abstract

The objective of this paper was to assess the antiradical effectiveness of propolis extract (PE) based on 2.2-diphenyl-1-picrylhydrazyl radical (DPPH) bleaching assay kinetic profile. The kinetic profile of scavenging DPPH for PE exhibited one kinetic period characterized by one kinetic constant. The second-order rate constant (k 2) for the oxidation of PE by DPPH, determined for the first time in this study, was 0.17 dm3g−1s−1. The obtained k2 value was compared to that of synthetic antioxidants and natural extracts used in the food industry. Kinetic analysis of PE antiradical effectiveness showed that the k 2 was within the range values for natural colorants of fruit extracts and should be considered as a fast acting natural antioxidant source. The k 2 parameter indicates the extent of oxidation inhibition that is based on all of the kinetic profiles of DPPH bleaching rather than single point measurements. For this reason, the kinetic analysis should become a necessary step for more precise antioxidative characterization of propolis.

Open access

Ljiljana Popović, Katarina Lalić, Olga Vasović, Danijela Drašković Radojković, Nataša Rajković, Sandra Singh, Ljubica Stošić, Miodrag Čivčić, Ljiljana Škorić Hinić and Tatjana Petrović Vujić

Summary

Background: Previous studies have indicated that high sensitivity C-reactive protein (hs-CRP) is a risk factor for the peripheral arterial disease (PAD) in diabetes. This study aimed to evaluate the possible predictive significance of hs-CRP for the development and progression of PAD in patients with type 2 diabetes (T2D).

Methods: The study included 80 patients previously diagnosed with T2D, aged 45–70 years, divided into group A (T2D patients with PAD; n=38) and group B (T2D patients without PAD; n=42). After five years, all the patients were re-examined and divided into subgroups depending on de novo development of PAD or progression of previously diagnosed PAD. Ankle-Brachial Index (ABI) measurement was used for PAD diagnosis and hs-CRP was determined by nephelometry.

Results: We found significantly higher hs-CRP levels in group A compared to group B, but only at baseline. Among the patients in group A, those with later progression of PAD (subgroup A1) had the highest levels of hs-CRP at baseline, although not significantly different from those in subgroup A2 (non-progressors). In contrast, hs-CRP level was significantly higher in subgroup B1 (progressors) in comparison to subgroup B2 (non-progressors) at both the first and second exam. Of all the investigated metabolic parameters, hs-CRP was the only independent predictor of PAD progression (OR=0.456, 95% CI=0.267–0.7815, p=0.004). The cut-off point for hs-CRP was 2.5 mg/L (specificity 75% and sensitivity 73.3%) with the relative risk for PAD of 2.93 (95% CI=1.351–6.3629).

Conclusions: Our study implies that hs-CRP can be used as a reliable predictor for the progression of PAD in patients with T2D.