Search Results

You are looking at 1 - 9 of 9 items for

  • Author: Jun Zheng x
Clear All Modify Search
Open access

Xiu-lan Zou, Jun Yang, Jian-ming Yang, Yan-qun Liu, Wen Wang, Jun Zheng, Bao-hua Ma and Fang-yuan Chen

Abstract

Background: Diabetic cardiomyopathy is an important complication of type 2 diabetics. The role of immunoinflammation, immunity, and diet has not been adequately clarified.

Objective: We investigated the relationships between diabetic cardiomyopathy and immuno-inflammation, as well as immunity and diets.

Methods: Sixty Sprague-Dawley (SD) male rats were included in this study, from which 12 were randomly selected as the normal control group (group A) and of which the remaining 48 were considered as the type 2 diabetes mellitus (T2DM) model group. Group A was fed with common diets and the T2DM model group, with high-glucose diets (by adding 20% cane sugar, 10% lard, and 2.5% cholesterol into a 67.5% common diet). After 4-week feeding, the T2DM model group was randomly allocated into three groups according to the diet, highfat diet group (group B), common diet group (group C), and low-fat diet group (group D). All the three groups were then fed for another 10 weeks. At the end of the experiment, body weight, random blood glucose levels, and cardiac weight were measured. Left ventricular tissue was obtained for light microscopy and electron microscopy. Deposits of immunoglobulin G (IgG) in myocardium were identified by immunohistochemistry. Serum levels of high-sensitivity C-reactive protein (hs-CRP) were determined using the enzyme-linked immunosorbent assay (ELISA). All data were statistically analyzed.

Results: The serum level of hs-CRP was significantly higher in groups B, C, and D, than in the control group. Therefore, IgG deposits among cardiac muscle cells were observed in all the model groups, significant deviations were noted in group A (p <0.01) and IgG deposits were less in group D than in groups B and C (p <0.01).

Conclusion: Immuno-inflammation participates in the development of T2DM and diabetic cardimyopathy. Immune injury can be alleviated following dietary interference.

Open access

Tie-long Zheng, Ping-an Wang, Dian-li Wang, Cheng-fu Sun, Yuan Hong, Qi Wang and Jun Cheng

Abstract

Objective To observe the biological function of human 3-hydroxyisobutyrate dehydrogenase (HIBADH).

Methods Human 3-hydroxyisobutyrate dehydrogenase (HIBADH, 3-hydroxy-2-methyl propanoate: NAD+ oxidoreductase) recombinant protein was expressed in E. coli BL21, and purified by Ni+ column. The special antisera was obtained from rabbits immunized by this purified antigen. On the distribution of HIBADH, it was found that HIBADH over-expressed in the injured liver cells when serious hepatitis occurred. The phenomenon was confirmed in the animal models of SD rats with acute liver cell injury induced by CCl4, but this phenomenon did not exist in the models induced by endotoxin combined with galactosamine. Further more, HIBADH’s overexpression in liver cells will induce cell necrosis through the pathway of oxidative stress.

Results When the liver cells injured by drug or other chemical materials, HIBADH will be compensationally over-expressed for the deficiency of energy, so liver cells can make enough ATP through brand-chain amino acid catabolism. However, the overexpression of HIBADH will be harmful for liver cells through the product of much more active oxygens which will induce the cell necrosis.

Conclusions HIBADH over-expression is a signal of the liver cell metabolism injury, and it can aggravate the liver cell injury through oxidative stress.

Open access

Ning Ma, Min Liu, Min Zheng, Xi Chen, Hong-yan Lu, Hong-guang Chen, Jun Zheng, Gui-ying Li, Juan Wang, Guo-wu Liu and Ning Wang

Abstract

Objective To estimate the HIV/AIDS epidemic and treatment demand and predict the situation in the next five years with Spectrum.

Methods Using Spectrum (version:3.54) to estimate the number of new HIV infections, number of people living with HIV, need for ART in adults and children, need for PMTCT and cotrimoxazole in Beijing and Hunan Province. Data used in the model including high-risk populations monitoring data and demographic information, was collected from Beijing Center for Disease Control and Prevention, Hunan Center for Disease Control and Prevention and extracted from statistical yearbooks and published literatures.

Results Few new HIV/AIDS were reported in Beijing prior to 1994, however, the number of HIV infections was increasing rapidly from 1995 to 2008, and decreased after that, increased rapidly again after 2012. The number of patients who need antiretroviral treatment was increasing among the group aged between 15-49 years with young patients aged 15-24 years peaked in 2010 and decreased rapidly after that. Few HIV-infected patients were reported before 1997 in Hunan Province, and the number of new infections was increasing rapidly since 1998. The number of patients who need antiretroviral treatment was increasing among the group aged between 15 - 49 years since 2000 with young patients aged 15-24 years in need of antiretroviral therapy increasing continuously.

Conclusions After HIV infection was first founded in Beijing and Hunan Province, there was a slow growth and then a rapid growth of HIV epidemic. According to prediction of Spectrum, the demand for antiretroviral therapy in Beijing would begin to decline since 2011, meanwhile, the HIV epidemic in Hunan Province would enter a rapid growth period with the demand for antiviral therapy continuing to increase. In this study, Spectrum was applied to estimate the HIV epidemic situation and need for treatment in the next five years in Beijing and Hunan Province. It provides the basis for health resource allocation and policy formulation of HIV management.

Open access

Zai-jun Xin, Xiu-zhen Li, Søren Nielsen, Zhong-zheng Yan, Yuan-qing Zhou, Yue Jia, Ying-ying Tang, Wen-yong Guo and Yong-guang Sun

Effect of Stubble Heights and Treatment Duration Time on the Performance of Water Dropwort Floating Treatment Wetlands (FTWS)

Floating treatment wetlands (FTWs) with Water Dropwort (Oenanthe javanica) were established in winter to investigate their potential role in the purification of eutrophicated water, and to identify the effects of different stubble heights of the Water Dropwort on the performance of the FTWs. The results of the experiments demonstrated: The Water Dropwort FTWs were effective in buffering the pH of the experimental water. The Water Dropwort FTWs were efficient in purifying eutrophicated water, with removal rate for total nitrogen (TN), total phosphorus (TP), ammonium nitrogen (NH4 + - N), and nitrate nitrogen (NO3 - - N) at 91.3, 58.0, 94.6, and 95.5% in the 15-day experiment, respectively. No significant difference in the purification effect was found among different stubble heights of Water Dropwort FTWs. Significant differences between the zero control and the FTWs were found for the removal of TP in the first 11 days; and for the removal of NH4 + - N in the first 4 days. No significant difference was found between the zero control and the FTWs for NO3 - - N in the first 4 days, but significant difference was detected after day 4. The optimum treatment duration time for the FTWs with Water Dropwort will depend on the nutrients to be removed. These results will provide basis for further application of the FTWs at large scale, as well as for future studies on the mechanism of nutrient removal process.

Open access

Chun-Ru Liu, Gong-Ming Yin, Hui-Ping Zhang, Wen-Jun Zheng, Pierre Voinchet, Fei Han, Duo Wang, Wei-Juan Song and Jean-Jacques Bahain

Abstract

The Minjiang River terrace along the Longmen Shan fault zone near Wenchuan, at the eastern margin of the Tibetan Plateau, China, provides archives for tectonic activity and quaternary climate change. However, previous studies were not able to provide ages older than 100 ka due to the limitations of dating material or/and methods applied to date the fluvial sediments. In this study, we used the ESR signal of the Ti-Li center in quartz to obtain the ages of four higher terraces (T3–T6). According to the results, the terraces T3 to T6 were formed at 64±19 ka, 101±15 ka, 153±33 ka, and 423±115 ka, respectively. Combined with previous studies, these results indicate that the formations of all terraces correspond to glacial/interglacial transition periods, such as, T1-T5 being correlated to MIS2/1, MIS4/3, MIS5d/5c, and MIS6/5e respectively, while T6 probably to MIS12/11. According to these data, it is found that the average incision rate was significantly higher over the last 150 ka than that previous 100 ka (250 to 150 ka). As both tectonics and climate have affected the formation of these terraces, in addition to the overall uplifting of Tibetan Plateau, the regional uplift due to isostasy would be an additional tectonic factor in the formation of river terraces in the eastern margin of Tibetan plateau.

Open access

Guoqing Wang, Jun Wang, Ming Li, Yaguang Zheng and Kai Wang

Abstract

Traditional image contrast enhancement methods originally cannot improve the quality of vein images and may also import some unknown noise resulting in low recognition rate. To overcome the abovementioned disadvantages, the paper proposes an enhancement method based on the morphological filtering theory including three main procedures. Firstly, the algorithm extract the vein Region Of Interest (ROI), and then adopting the improved White Top-Hat transform (WTH) and Black Top-Hat transform (BTH) methods to get the features of vein in detail in both white and black pattern (vein information and background information); Secondly, to construct the filtering function with the self-designed controlling operator, representing the gradient changes of the vein edges, which well reflects the importance of local detail in multi-scale pattern; Finally, traditional nonlinear gray-level transformation function is imported with modality to the parameters to realize the gray normalization. We perform rigorous experiments with the proposed method and other state-of-the-art enhancement methods on the self-built dorsal vein image databases, and the experimental results illustrate that the multiscale top-hat theory-based enhancement methods improve the contrast of hand vein images with restrictions on the possibility of enhancement on existing noise information.

Open access

Shengdong Zhu, Pei Yu, Mingke Lei, Yanjie Tong, Lu Zheng, Rui Zhang, Jun Ji, Qiming Chen and Yuanxin Wu

Ionic liquid (IL) pretreatment of lignocellulosic materials has provided a new technical tool to improve lignocellulosic ethanol production. To evaluate the influence of the residual IL in the fermentable sugars from enzymatic hydrolysis of IL pretreatment of lignocellulosic materials on the subsequent ethanol fermentation, the toxicity of the IL 1-butyl-3-methylimidazolium chloride ([BMIM]Cl) to Saccharomyces cerevisiae AY93161 was investigated. Firstly, the morphological structure, budding and metabolic activity of Saccharomyces cerevisiae AY93161 at different [BMIM]Cl concentrations were observed under an optical microscope. The results show that its single cell morphology remained unchanged at all [BMIM]Cl concentrations, but its reproduction rate by budding and its metabolic activity decreased with the [BMIM]Cl concentration increasing. The half effective concentration (EC50) and the half inhibition concentration (IC50) of [BMIM]Cl to Saccharomyces cerevisiae AY93161 were then measured using solid and liquid suspension culture and their value were 0.53 and 0.39 g.L-1 respectively. Finally, the influence of [BMIM]Cl on ethanol production was investigated. The results indicate that the [BMIM]Cl inhibited the growth and ethanol production of Saccharomyces cerevisiae AY93161. This toxicity study provides useful basic data for further development in lignocellulosic ethanol production by using IL technology and it also enriches the IL toxicity data.

Open access

Xin Yao, Ting Wu, Cheng Zhou, Yi-min Li, Feng-cai Zhu, Qiang Yan, Wei-jin Huang, Chuan Ji, Zheng-lun Liang and Jun-zhi Wang

Objective To investigate the prevalence and levels of anti-HEV IgG in the population of Jiangsu Province.

Methods Total of 2 656 samples from Qindong and 11 463 samples from Anfeng were colleted. The anti- HEV antibody was qualitatively and quantitatively detected using ELISA kits and the references had been established.

Results The positive rates of anti-HEV IgG in male and female were 55.6% and 40.1%, respectively. The positive rate of anti-HEV IgM in male and female were both 3.4%. In opposite to anti-HEV IgG, the positive rate of anti-HEV IgM in Anfeng was significant higher than that in Qindong. The mean anti-HEV IgG titers for 6 age groups were 0.94, 0.92, 1.07, 1.46, 1.27, 1.19 and 0.68, 1.31, 1.08, 1.14, 1.31, 1.68 IU/ml, in Qindong and Anfeng region, respectively. The positive rate of anti-HEV IgG tended to increase with age and the titer of anti- HEV IgG was associated with age (R > 0.90).

Conclusions The results in this study showed that HEV was widely prevalent in both Qindong and Anfeng of Jiansu Province and the prevalence and the anti-HEV IgG titer were associated with gender and age.

Open access

Wen Xie, Hong Zhao, Yu Chen, Qin Zhang, Wei Lu, Wei Liu, Ai-rong Hu, Han-wei Li, Ping Feng, Ming-sheng Chen, Cun-jin Mei, Xiao-lin Guo, Xiao-hu Zhao, Jiang-bin Wang, Zheng-qin Fan, Jian-he Gan, Qing Xie and Jun Cheng

Abstract

Obejective Ademetionine 1,4-butanedisulfonate [S-adenosyl-L-methionine (SAMe)/Transmetil®, Abbott] has been available in China for more than 15 years, and it has been shown to reduce serum bilirubin and transaminase levels in viral hepatitis (VH) patients. However, no large-scale studies have focused on the impact of SAMe treatment regimen on reducing the serum total bilirubin (TBil) in VH patients with intrahepatic cholestasis (IHC). The primary purpose of this study was to evaluate the effectiveness of intravenous SAMe (Transmetil®) treatment in reducing the serum TBil by 50%.

Methods This retrospective, multi-center, cross-sectional medical record review involved patients aged ≥18 years. Records of 1 280 hospitalized VH patients at 16 sites diagnosed with IHC who had received intravenous SAMe 1 000 mg or 2 000 mg q.d. for at least 7 days from January 1, 2006 to June 30, 2009, were screened and 905 records were randomly selected.

Results The safety set (SS) included 834 patients and the full analysis set 826 patients. TBil levels after 14 days injection treatment were available for 763 patients. TBil decreased ≥ 50% versus baseline after 14 days treatment in 288 (37.7%) patients (95% CI 34.3%, 41.2%). Twenty-nine non-serious adverse events (non-SAEs) were reported in 19 (2.3%) patients, and 29 SAEs were reported in 10 patients (1.2%). All adverse events (AEs) were considered unrelated to the study drug.

Conclusions This retrospective study shows that intravenous SAMe administration in VH patients with IHC is associated with significant reduction of TBil levels in more than 30% of patients 14 days after treatment initiation.