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Eun-Joo Lee, Kim Ah-Young, Lee Eun-Mi, Park Jin-Kyu and Jeong Kyu-Shik

Abstract

Malignant pilomatricoma is malignant follicular tumor with only matrical differentiation. Malignant pilomatricoma is rare in dogs. There is little information about sex, breed and age predisposition. There are a few reports of canine malignant pilomatricoma in middle to old age dogs. However, this neoplasm was resected from 1-year-old intact male miniature poodle. The neoplasm was found in the dorsal part of the neck. The mass was firm and protruded. On gross findings the size of the mass was 3×2×1.5cm. The mass was located in the deep dermis and subcutaneous layer. The mass was composed of several lobules of grey-white chalky material. Microscopically, the mass was composed of several large and small lobules. There were basophilic round to oval basaloid cells at the periphery of the lobules. The basophilic cells showed abrupt keratinization. Numerous ghost cells were observed in the center of the mass. The ghost cells had abundant eosinophilic cytoplasm without a nucleus. The basophilic basaloid cells showed numerous atypical mitotic figures. Cellularity was high and pleomorphism was remarkable. No lymphatic metastasis was observed. We reported a rare case of malignant pilomatricoma in a 1-year-old young dog.

Open access

Jeong Hwa Kim, Jin Kyu Park and Jae Kwon Lee

Abstract

Nosemosis is one of the most common protozoan diseases of adult bees (Apis mellifera). Nosemosis is caused by two species of microsporidia; Nosema apis and Nosema ceranae. Nosema ceranae is potentially more dangerous because it has the ability to infect multiple cell types, and it is now the predominant microsporidian species in A. mellifera. In this study, we identified two anti-nosemosis plants, Aster scaber and Artemisia dubia, which reduced the spore development of N. ceranae in spore-infected cells. The most important aspect of our results was that our treatment was effective at non-toxic concentrations. Anti-nosemosis activities of both plants were revealed in honey bee experiments. Specifically, a mixed extract of both A. scaber and A. dubia showed stronger activity than treatment with each single extract alone. Although the mechanisms of action of A. scaber and A. dubia against N. ceranae are still unclear, our results suggest new medicaments and therapeutic methods to control N. ceranae infection.

Open access

Jae Kwon Lee, Jeong Hwa Kim, Mina Jo, Balamurugan Rangachari and Jin Kyu Park

Abstract

In our previous study, we demonstrated that the ethanol extracts of Artemisia dubia (A. dubia) and Aster scaber (A. scaber) have anti-nosemosis activity. In our present study, we intend to establish the anti-nosemosis activity of aqueous, ethyl acetate (EA), and butanol (BuOH) extracts of A. dubia and A. scaber. In order to determine the optimal dose, we performed both in vitro and in vivo toxicity for all the extracts and also carried out anti-nosemosis experiments. Although all of the extracts (aqueous, EA, and BuOH) showed in vitro and in vivo anti-nosemosis activity in a dose-dependent manner, the aqueous extracts of A. dubia and A. scaber showed more potent anti-nosemosis activity than the EA and BuOH extracts. Moreover, an aqueous extract of A. dubia + A. scaber demonstrated stronger anti-nosemosis activity compared with the aqueous extracts of either A. dubia or A. scaber alone. Although the main ingredients in A. dubia and A. scaber remain unclear, our results suggest that the active components of A. dubia and A. scaber could dissolve in the aqueous fraction.

Open access

PARK Byung-Yong, SHIM Kwan-Seob, KIM Won-Il, HOSSAIN Md Mukter, KIM Bumseok, KWON Jungkee, PARK Choi-Kyu, CHO Sung-Jin, JO Inho and CHO Ho-Seong

Abstract

A simple and rapid real-time loop-mediated isothermal amplification (LAMP) assay designed to detect Lawsonia (L.) intracellularis, an important bacteria causing proliferative enteropathy in pigs. A set of four primers targeting the ubiquinone/menaquinone biosynthesis methylase (ubiE) gene was designed for the LAMP reaction. Additionally, serial 10-fold dilutions of cultured L. intracellularis and spiked feces were also used for the optimization of real-time LAMP. The lower limit of the linear range of the assay in L. intracellularis was 1.0 × 100 L. intracellularis. Real-time LAMP was 10 and 100 times more sensitive than real-time PCR and conventional PCR detection methods, respectively. Based on testing of 213 porcine fecal samples using real-time LAMP, realtime PCR and PCR, the agreement quotients of real-time LAMP with conventional PCR and with real-time PCR were 0.77 and 0.95, respectively. This study demonstrated that real-time LAMP was a powerful tool for the rapid and sensitive detection of L. intracellularis in porcine fecal samples.