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Jie Xu, Jun Shen, Yue Ding, Hui-Yong Shen, Zhan-Peng Zeng, Ruo-Fan Ma, Chun-Hai Li and Bertram Barden

The clinical value of combined use of MR imaging and multi-slice spiral CT in limb salvage surgery for orthopaedic oncology patients: initial experience in nine patients

Background. The purpose of this prospective study was to evaluate the value of the combined use of MR imaging and multi-slice spiral CT for limb salvage surgery in orthopaedic oncology patients.

Patients and methods. Nine consecutive patients with lower/upper limb malignant bone tumours (7 osteosarcomas and 2 chondrosarcomas) were treated with limb-salvaging procedures. Preoperative planning including determination of the osteotomy plane and diameters of the prosthesis was performed basing on the preoperative CT and MR images. The histopathology was performed as golden diagnostic criteria to evaluate the accuracy of CT and MR-based determination for tumour's boundary.

Results. The tumour extension measured on MRI was consistent with the actual extension (P>0.05, paired Student's t test), while the extension measured on CT imaging was less than the actual extension. The length, offset and alignment of the affected limb were reconstructed accurately after the operation. An excellent functional outcome was achieved in all patients.

Conclusions. In the present study, MRI was found to be superior to CT for determining the tumour extension, combined use of MRI and CT measurement provided high precision for the fit of the prosthesis and excellent functional results.

Open access

Jie Shen, Kai Yang, Caihua Sun and Minxia Zheng


Correlation analysis based on chromatograms and pharmacological activities is essential for understanding the effective components in complex herbal medicines. In this report, HPLC and measurement of antioxidant properties were used to describe the active ingredients of Salvia miltiorrhiza injection (SMI). HPLC results showed that tanshinol, protocatechuic aldehyde, rosmarinic acid, salvianolic acid B, protocatechuic acid and their metabolites in rat serum may contribute to the efficacy of SMI. Assessment of antioxidant properties indicated that differences in the composition of serum powder of SMI caused differences in vascular endothelial cell protection. When bivariate correlation was carried out it was found that salvianolic acid B, tanshinol and protocatechuic aldehyde were active components of SMI because they were correlated to antioxidant properties.

Open access

Jing Shen, Xiao-Ming Lei, Yang Song, Xing Tan, Qin Liu, Li-Wen Dai and Jie Yu


Objective: To observe the effects of electro-acupuncture (EA) on GRP78 and Caspase-12 gene expression in rats with ischemia- reperfusion injury (IRI) by stimulation on Nei Guan (PC6) and Bai Hui (GV20) points, so that to understand whether or not the protective effects of acupuncture is related to endocytoplasmic reticulum (ER) stressapoptosis passage.

Methods: 50 rats were randomly assigned to five groups (10 in each group): normal control(A), pseudo-operation(B), operation(C), Edaravone(D) and EA(E). The ischemia/reperfusion model of middle cerebral artery occlusion (MCAO) was established by suture embolic method. TUNEL staining method was employed to measure the apoptosis index of nerve cells in rats. Real-time polymerase chain reaction (RT-PCR) was employed to measure the mRNA expression of GRP78 and Caspase-12.

Results: Compared with normal group and pseudo-operation group, the apoptosis indexes and mRNA expression of GRP78 and Caspase-12 in operation group, Edaravone group and EA group were increased, with statistical significance(P<0.05 or P<0.01); compared with operation group, the apoptosis indexes and Caspase-12 mRNA expression in Edaravone group and EA group were decreased(P<0.05 or P<0.01), but GRP78 mRNA expression were increased(P<0.01); there were no significant difference between Edaravone group and EA group on the above indexes(P>0.05).

Conclusion: Acupuncture on Nei Guan and Bai Hui points could effectively suppress the nerve cell apoptosis in cerebral ischemia. The underlying mechanism might be related to upregulation of the ERS-protective GRP78 expression and downregulation of apoptosis-promotion Caspase-12 expression.

Open access

Jing Shen, Chao Wang, Jia-Li Zhang, Zhao Liu, Mi Liu, Jie Yan, Xiao-Rong Chang and li-Na Guo


Objective: To investigate the influence of electroacupuncture (EA) preconditioning at Nei Guan point (PC6) on the opening of mitochondrial permeability transition pore (MPTP) of rabbits with myocardial ischemia-reperrfusion injury, and its underlying mechanism that protects myocardium from injury.

Methods: 18 New Zealand rabbits were randomly assigned to pseudo-operation, model and EA groups, 6 in each group. Setting up models by ligation of coronary artery and electrically stimulating the rabbits in EA group 20 min per day in 5 days before modeling. Testing the activity of serum SOD with the method of actinochemistry, testing changes of mitochondrial transmembrane potential of myocardial cells with the method of synchronous spectrometry, using spectrophotometry to test the changes of the absorption of mitochondria at the spectrum of 520nm to check the opening of MPTP, and testing the apotosis of cells by means of terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL).

Results: Compared with model groups, the SOD activity of myocardial cells was significantly enhanced, and the apotosis index (AI) was reduced(P<0.01),mitochondrial transmembrane potential was significantly increased (P<0.05),absorption of mitochondria was significantly decreased(P<0.01).

Conclusion: EA can remarkably improve myocardial ischemia - reperfusion injury, and protect myocardium.